The effect of UV-B radiation on the quantity and quality of essential oils and genetic stability in Ocimum Basilicum L.
محورهای موضوعی : Research On Crop Ecophysiology
Sahar Abbasi
1
(گروه زیست شناسی دانشگاه آزاد اسلامی واحد ساوه )
مریم پیوندی
2
()
حسین عباسپور
3
(گروه زیست شناسی دانشگاه آزاد اسلامی واحد دامغان )
Mahdis Ebrahimzadeh
4
(عضو هیئت علمی و مدیر گروه دانشگاه ازاد اسلامی واحد کرج)
احمد مجد
5
(دانشگاه خوارزمی تهران)
کلید واژه: Ocimum Basilicum L., UV radiation, Genetic stability, Essential oil.,
چکیده مقاله :
Ultraviolet B (UV-B) radiation had deleterious effects on plants. The purpose of this study was to investigate the effect of UV B irradiation time period on genetic variation and quantity and quality of essential oils of Ocimum Basilicum L.. Seeds were exposed to 0.38 W/m2 of UV-B radiation for 0, 7, 15, 30 days (ten minutes each day). The GC-MS results showed UVB treatments brought about distinct alternations in quality of essential oils of treated plants. Estragol, Eucalyptol, Caryophyllene, Bisabolene, Cubebene and Methyleugenol were identified as major of the compounds. The results of Ward clustering based on essential oils indicated that the mid- time irradiation treatment had the highest similarity to the essential oil composition with control plants. To evaluate the genetic variation in treated plants, 6 ISSR primers were used. The molecular markers used indicated that the 7-day control and treatment samples were significantly separated from the 15 and 30-day treatments of the basil plant samples by UV irradiation, respectively. The UPGMA dendrogram and plots from PCoA analyzes separated the different treatment groups. So that the 7-day control and control samples were significantly separated from the 15 and 30-day UV treated of the basil plant samples, respectively. It can be finally concluded that the UV-B treatment can significantly affect the genetic materials beside of the essential oils content of basil, especially in the long time exposures.
Ultraviolet B (UV-B) radiation had deleterious effects on plants. The purpose of this study was to investigate the effect of UV B irradiation time period on genetic variation and quantity and quality of essential oils of Ocimum Basilicum L.. Seeds were exposed to 0.38 W/m2 of UV-B radiation for 0, 7, 15, 30 days (ten minutes each day). The GC-MS results showed UVB treatments brought about distinct alternations in quality of essential oils of treated plants. Estragol, Eucalyptol, Caryophyllene, Bisabolene, Cubebene and Methyleugenol were identified as major of the compounds. The results of Ward clustering based on essential oils indicated that the mid- time irradiation treatment had the highest similarity to the essential oil composition with control plants. To evaluate the genetic variation in treated plants, 6 ISSR primers were used. The molecular markers used indicated that the 7-day control and treatment samples were significantly separated from the 15 and 30-day treatments of the basil plant samples by UV irradiation, respectively. The UPGMA dendrogram and plots from PCoA analyzes separated the different treatment groups. So that the 7-day control and control samples were significantly separated from the 15 and 30-day UV treated of the basil plant samples, respectively. It can be finally concluded that the UV-B treatment can significantly affect the genetic materials beside of the essential oils content of basil, especially in the long time exposures.
REFERENCES
1. Weihs, P., A.W. Schmalwieser, and G. Schauberger, UV EffectsUV (ultraviolet)effectson Living Organisms, in Encyclopedia of Sustainability Science and Technology, R.A. Meyers, Editor. 2012, Springer New York: New York, NY. p. 11375-11427.
2. van de Staaij, J., et al., Flavonoid concentrations in three grass species and a sedge grown in the field and under controlled environment conditions in response to enhanced UV-B radiation. Journal of Photochemistry and Photobiology B: Biology, 2002. 66(1): p. 21-29.
3. Schmalwieser, A.W., P. Weihs, and G. Schauberger, UV Effects on Living Organisms, in Encyclopedia of Sustainability Science and Technology, R.A. Meyers, Editor. 2019, Springer New York: New York, NY. p. 1-63.
4. Moreno, N.C., et al., ATR/Chk1 Pathway is Activated by Oxidative Stress in Response to UVA Light in Human Xeroderma Pigmentosum Variant Cells. Photochemistry and Photobiology, 2019. 95(1): p. 345-354.
5. Simontacchi, M., et al., Plant Survival in a Changing Environment: The Role of Nitric Oxide in Plant Responses to Abiotic Stress. Frontiers in plant science, 2015. 6: p. 977-977.
6. Jiang, Y., et al., Regulation of Floral Terpenoid Emission and Biosynthesis in Sweet Basil (Ocimum basilicum). Journal of plant growth regulation, 2016. 35(4): p. 921-935.
7. Vassao, D., et al., Chavicol formation in sweet basil (Ocimum basilicum): Cleavage of an esterified C9 hydroxyl group with NAD(P)H-dependent reduction. Organic & biomolecular chemistry, 2006. 4: p. 2733-44.
8. Hikosaka, S., K. Ito, and E. Goto, Effects of Ultraviolet Light on Growth, Essential Oil Concentration, and Total Antioxidant Capacity of Japanese Mint. Environmental Control in Biology, 2010. 48(4): p. 185-190.
9. Sarahroodi, S., et al., The effects of green Ocimum basilicum hydroalcoholic extract on retention and retrieval of memory in mice. Ancient science of life, 2012. 31(4): p. 185-189.
10. Chang, X., P. Alderson, and C. Wright, Enhanced UV-B radiation alters basil (Ocimum basilicum L.) Growth and stimulates the synthesis of volatile oils. Journal of Horticulture and Forestry, 2009. 1: p. 27-31.
11. Hikosaka, S., K. Ito, and E. Goto, Effects of Ultraviolet Light on Growth, Essential Oil Concentration, and Total Antioxidant Capacity of Japanese Mint. Environment Control in Biology, 2010. 48: p. 185-190.
12. Ioannidis, D., L. Bonner, and C.B. Johnson, UV-B is required for normal development of oil glands in Ocimum basilicum L. (sweet basil). Annals of botany, 2002. 90(4): p. 453-460.
13. Takshak, S. and S. Bhushan Agrawal, Interactive effects of supplemental ultraviolet-B radiation and indole-3-acetic acid on Coleus forskohlii Briq.: Alterations in morphological-, physiological-, and biochemical characteristics and essential oil content. Ecotoxicology and Environmental Safety, 2018. 147: p. 313-326.
14. MAJD, A., et al., THE EFFECT OF ULTRAVIOLET RADIATION ON THE ANATOMICAL STRUCTUR, QUANTITY AND QUALITY OF THE ESSENTIAL OILS OF DIFFERENT ORGANS OF FOENICULUM VULGARE MILER. (FENNEL). IRANIAN JOURNAL OF BIOLOGY, 2002. 12(1-2): p. -.
15. Evans, J.R., Improving photosynthesis. Plant physiology, 2013. 162(4): p. 1780-1793.
16. Palma, C.F.F., et al., Effects of UV radiation on transcript and metabolite accumulation are dependent on monochromatic light background in cucumber. Physiologia Plantarum, 2021. 173(3): p. 750-761.
17. Yan, Y., et al., The transgenerational effects of solar short-UV radiation differed in two accessions of Vicia faba L. from contrasting UV environments. Journal of Plant Physiology, 2020. 248: p. 153145.
18. Müller, R., et al., UV-B Exposure of Black Carrot (Daucus carota ssp. sativus var. atrorubens) Plants Promotes Growth, Accumulation of Anthocyanin, and Phenolic Compounds. Agronomy, 2019. 9(6): p. 323.
19. Johnson, C.B., et al., Substantial UV-B-mediated induction of essential oils in sweet basil (Ocimum basilicum L.). Phytochemistry, 1999. 51(4): p. 507-510.
20. Huarancca Reyes, T., et al., Physiological responses of maca (Lepidium meyenii Walp.) plants to UV radiation in its high-altitude mountain ecosystem. Scientific Reports, 2020. 10(1): p. 2654.
21. Hashemi, M.B., M. Niakousari, and M.J. Saharkhiz, Antioxidant activity of Satureja bachtiarica Bunge essential oil in rapeseed oil irradiated with UV rays. European Journal of Lipid Science and Technology, 2011. 113(9): p. 1132-1137.
22. Righini, S., et al., Apigenin produced by maize flavone synthase I and II protects plants against UV-B-induced damage. Plant, Cell & Environment, 2019. 42(2): p. 495-508.
23. Höll, J., et al., Impact of pulsed UV-B stress exposure on plant performance: How recovery periods stimulate secondary metabolism while reducing adaptive growth attenuation. Plant, Cell & Environment, 2019. 42(3): p. 801-814.
