اثر سیستم آبیاری و دو سویه باکتری محرک رشد گیاه (PGPR) بر تركيبات فنلي و فعاليت آنتي-اكسيداني عصاره کلاله زعفران
محورهای موضوعی : زیست شناسی سلولی تکوینی گیاهی و جانوری ، تکوین و تمایز ، زیست شناسی میکروارگانیسم
reza dehghani
1
(university of kashan)
کلید واژه: فلاونوئید, باکتری محرک رشد, عصاره, آبیاری قطره¬ای, زعفران,
چکیده مقاله :
تركيبات فنلي (فلاونوئيد، تانن و آنتوسيانين) مهم¬ترين آنتي¬اكسيدان¬های طبيعي به شمار مي¬آيند و عوامل متعددی مانند عوامل محیطی و تغذیه گیاه بر کمیت و کیفیت آنها تاثیرگذارمی¬باشند. یکی از گیاهان دارویی بومی ایران، زعفران با نام علمی Crocus sativus Var. officinalis است که سابقه استفاده از ترکیبات آن به زمان¬های کهن بر می¬گردد و در مناطق مختلف کشور به صورت طبیعی و کشت شده وجود دارد. تحقيق حاضر به منظور بررسي كمي و كيفي تركيبات فنلي و فعاليت آنتي¬اكسيداني عصاره زعفران تحت تاثیر دو سویه باکتری محرک رشد (ازتوباکتر کروکوکوم سویه 5) و (سودوموناس فلورسنس سویه 169) و همچنین دو روش¬ آبیاری غرقابی و قطره¬ای انجام شده است. در اين مطالعه آزمايشگاهي ابتدا بررسي فيتوشيمي گياه انجام شد، سپس سنجش ميزان ترکیبات فنلي و فلاونوئيدي تام به روش اسپكتروفتومتري صورت گرفت و در نهايت فعاليت آنتي¬اكسيداني عصاره گياه در غلظت¬هاي مختلف با استفاده از روش مهار راديكال آزاد 2 و 2 دي فنيل 1- پيكريل هيدرازيل (DPPH) اندازه گیری شد. تجزيه و تحليل داده¬ها با نرم افزار SPSS نسخه 19 و روش آنالیز واريانس انجام شد. همچنین میزان ترکیبات فلاونوئیدی موجود در نمونه¬ای که ازتوباکتر کروکوکوم سویه 5 به کار برده شده بود کمی بیش¬تر از سایر نمونه¬ها بود. تیمار سودوموناس فلورسنس سویه 169 و تیمار آبیاری غرقابی به¬ ترتیب بیش¬ترین و کم¬ترین میزان ترکیبات فنلی و تیمار آبیاری قطره¬ای بیش¬ترین خاصیت آنتی¬اکسیدانی را از خود نشان دادند. لذا کاربرد باکتری¬های محرک رشد و جایگزینی سیستم آبیاری قطره¬ای در کشت زعفران پیشنهاد می¬گردد.
Phenolic compounds (flavonoids, tannins, and anthocyanin) are considered as most important natural antioxidants and many factors such as environmental parameters and plant nutrition affect their quantity and quality. One of the native medicinal plants of Iran is saffron with the scientific name of Crocus sativus Var. officinalis. That the use of its compounds be traced to ancient times and there is natural and cultivated in the different regions of the country. This study is done with aims to evaluate the quality and quantity of phenolic compounds and antioxidant activity Saffron species under the influence of two types of PGPR (Azotobacter chroococcum 5 and pseudomonas fluorescens 169) and two irrigation systems (flooding and drip). In this study, plant phytochemistry study was conducted initially, and then measuring a number of phenolic compounds and flavonoids were spectrophotometry method. Finally, the antioxidant activity of the extract was measured in different concentrations using free radical scavenging (DPPH). Data analysis was performed by using SPSS version 19 software and ANOVA test. The results of phytochemical tests confirmed the compounds such as tannins, anthocyanins and flavonoids and absence of alkaloids. A number of flavonoid compounds in the sample with Azotobacter chroococcum 5 was more than other samples. pseudomonas fluorescens 169 and flooding treatments had the highest and lowest number of phenolic compounds and drip irrigation showed the highest antioxidant properties. Therefore, the use of growth-stimulating bacteria and replacing the drip irrigation system in saffron cultivation is suggested
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