اثر پیش تیمار با نانو کلات آهن و سالیسیلیک اسید بر جوانه زنی و رشد عدس تحت تنش شوری

محورهای موضوعی :

حسن نورافکن ¹ (عضو باشگاه پژوهشگران جوان و نخبگان دانشگاه آزاد اسلامی واحد میانه میانه، ایران)
معصومه شاهمرادی ² (عضو باشگاه پژوهشگران جوان و نخبگان دانشگاه آزاد اسلامی واحد میانه میانه، ایران)

تاریخ دریافت : 1393/02/18 تاریخ پذیرش : 1393/06/07 تاریخ انتشار : 1393/06/01

کلید واژه: پرایمینگ, Lens culinaris, شبه هورمون, کلرید سدیم, هیدروپرایمینگ, نانو فن آوری,

چکیده مقاله :

به منظور بررسی اثر پیش تیمار بذر با نانو کلات آهن و سالیسیلیک اسید بر خصوصیات جوانه زنی و رشد عدس تحت تنش شوری، آزمایشی به صورت فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار انجام شد. تیمارهای آزمایش شامل پیش تیمار با سالیسیلیک اسید و نانو کلات آهن هر کدام در سه سطح شامل 1000، 2000 و 3000 میلی گرم بر لیتر، پیش تیمار با آب مقطر و شاهد (بدون اعمال پیش تیمار) و چهار سطح شوری شامل شاهد، 1000، 2000 و 3000 میلی گرم بر لیتر بود. نتایج نشان داد که اثر پیش تیمار با نانو کلات آهن و سالیسیلیک اسید در شرایط شوری بر درصد و سرعت جوانه زنی، درصد و سرعت سبز شدن، میانگین مدت زمان جوانه زنی و سبز شدن، طول ساقه چه، وزن تر و وزن خشک ساقه چه در سطح احتمال 1% معنی دار بود. غلظت 3000 میلی گرم بر لیتر کلرید سدیم سبب کاهش معنی دار شاخص های جوانه زنی و رشد گیاهچه نسبت به شاهد گردید. در کل، سالیسیلیک اسید نسبت به نانو کلات آهن اثر مثبت بیشتری بر شاخص های جوانه زنی و رشد گیاهچه عدس در شرایط تنش و بدون تنش شوری داشت و با کاربرد سالیسیلیک اسید به تنهایی و تحت تنش شوری شاخص های جوانه زنی و رشد گیاهچه نسبت به شاهد افزایش یافت. بنابراین پیش تیمار بذر با سالیسیلیک اسید 3000 میلی گرم بر لیتر موجب بهبود جوانه زنی و استقرار گیاهچه در شرایط شوری و بدون شوری می شود.

چکیده انگلیسی:

To study the effect of seed priming by salicylic acid and nano-iron chelate on germination and initial growth of lentil under salinity stress, a factorial experiment was conducted based on completely randomized design with three replications. Treatments was contained priming with salicylic acid and nano-iron chelate each with three levels (1000, 2000 and 3000 mg.L-1), hydropriming with distilled  water and control (non-priming) and four salinity levels (control, 1000, 2000 and 3000 mg.L-1). The results showed that seed priming by salicylic acid and nano-iron chelate had significant effect on germination percentage and rate, emergence percentage and rate, mean of germination and emergence time mean, plumule length, fresh and dry weight under salinity stress. The sodium chloride salt with 3000 mg.L-1 rate caused significant decrement in seedling germination and initial growth characteristics, comparing to control. In the present study, salicylic acid showed positive effect on germination and growth characteristics of lentil seedling under salinity stress with and without nano-iron chelate, so that germination and initial growth characteristics of lentil seedling were increased by application of salicylic acid in sole and under salinity stress, comparing to control. Therefore, applying seed priming by salicylic acid with 3000 mg.L-1 to improve germination and establishment of seedling with or without salinity stress condition is going to be recommended.

منابع و مأخذ:

1. Afzal I (2005) Seed enhancements to induced salt tolerance in wheat (Triticum aestivum L.). Ph.D. Thesis. Agricultural University of Faisalabad, Pakistan.


2. Ashraf M, Waheed G (1990) Screening of local lexoric of lentil (Lens culinaris Mendik) for salt tolerance at two growth stages. Plant and Soil 110: 63-67.


3. Cachorro P, Ortiz A (1993) Growth, water relations and solute composition of Phaseolus vulgaris, under saline conditions. Plant Science 95: 23-29.


4. El-Tayeb MA (2005) Response of barley Gains to the interactive effect of salinity and salicylic acid.Plant Growth Regulation 45: 215-225.


5. Fariduddin Q, Hayat S, Ahmad A (2003) Salicylic acid influences net photosynthetic rate, carboxylation efficiency, nitrate reductase activity and seed yield in Brassica juncea. Photosynthetica 41(2): 281-284.


6. Farooq M, Basra SMA, Tabassum R, Ahmed N (2006) Evaluation of seed vigor enhancement techniques on physiological and biochemical basis in coarse rice (Oryza sativa L.). Seed Science and Technology 34: 741-750.


7. Fathi-Amirkhiz K, Omidi H, Heshmati S, Jaefarzadeh L (2012) Study the effect of catalytic on seed vigor and germination characteristics of herb Nigella (Nigella sativa L.) under salt stress. Mashhad Ferdousi University. Iranian Journal of Field Crops Research 10(2): 299-310. [In Persian].


8. Hanan ED (2007) Influence of salicylic acid on stress tolerance during seed germination of Triticum aestivum and Hordeum vulgare. Biological Research 1: 40-48.


9. Jamil M, Rha ES (2004) The effect of salinity (NaCl) on the germination and seedling of sugar beet (Beta Vulgaris L.) and cabbage (Brassica oleracea capitita L.). Korean Resource 7: 226-232.

10. Jamil MC, Lee Rehman SU, Lee DB, Ashraf M, Rha ES (2005) Salinity tolerance of Brassica species at germination and early seedling growth. Journal of Environmental, Agricultural and Food Chemistry 4(4): 970-976.

11. Kang C, Wang Ch (2003) Salicylic acid changes activities of H₂O₂ metabolizing enzymes and increases the chilling tolerance of banana seedlings. Environment and Experimental Botany 9-15.

12. Kattab H (2007) Role of glutathione and polyadenylic acid on the oxidative defense systems of two different cultivars of canola seedlings grown under saline conditions. August. Journal of Basic Application Science 1: 323-334.

13. Kaya MD, Okcu G, Atak M, Cikili Y, Kolsarici O (2006) Sees treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). European Journal of Agronomy 24: 291-295.

14. Khan HA, Ayub CM, Pervez MA, Bilal RM, Shahid MA, Ziaf K (2009) Effect of seed priming with NaCl on salinity tolerance of hot pepper (Capsicum annuum L.) at seedling stage. Soil and Environment 28: 81-87.

15. Khosh-Khui M (2005) Plant propagation: Principle and practices. Shiraz University Press. 378 pp. [In Persian with English abstract].

16. Krnzhady A, Galeshi S, Zeinali A, Zangi MR (1999) Evaluation of cotton genotypes for salt tolerance at germination stage. Journal of Agricultural Science and Technology 18: 109-126.

17. Kulkarni MG, Street RA, Staden JV (2007) Germination and seedling growth requirements for propagation of Diosscorea dregeana (Kunth) Dur. And Schinz-A tuberous medicinal plant. South African Journal of Botany 33: 131-137.

18. Maddah M (2001) Effect of salicylic acid on some aspects of developmental, genetic, functional and resistant pea plant (Cicer arietinua) in natural conditions and cultured on glass. Ph.D. theses. Islamic Azad University. Science and Research Branch.

19. Maia FC, Moraes DM (2000) Salicylic acid: quality effects of soybean seeds.Revista Brasileira de sementes 22:264-270.

20. Manchanda G, Garg N (2008) Salinity and its effects on the functional biology of legumes. Acta Physiology Plant 30: 595-618.

21. Mazaheri-Tirani M, Manoochehri-Kalantari Kh (2006) Study of three factors salicylic acid, dry stress and ethylene and their interaction on seed germination of canola (Brassica napus L.). Journal of Iranian Biology 9: 408-418. [In Persian].

22. Mohammadi M, Fahimi H, Majd A (2007) Comparison of the effect of salicylic acid and giberlic acid on germination rate of lentils (Lens culinaris L.). Journal of Biology. Islamic Azad University, Garmsar Branch 4(4): 33-44.

23. Muhammad Z, Hussain F (2010) Vegetative growth performance of five medical plants under NaCl salt stress. Pakistan Journal of Botany 42: 303-316.

24. Nun NB, Plakhine D, Joel D, Mayer A (2003) Changes in the activity of the alternative oxidase in Orobanche seeds during conditioning and their possible physiological function. Phytochemistry 64: 235–241.

25. Pandya DH, Mer RK, Prajith PK, Pandy AN (2004) Effect of salt stress and manganese supply on growth of barely seeding. Journal of Plant Nutrition 27(8): 1361-1379.

26. Panwar P, Bhardwaj SD (2005) Handbook of practical forestry. Agrobios (INDIA). 191 PP.

27. Parida AK, Das AB (2005) Salt tolerance and salinity effects on plants: a review. Ecotoxicology and Environmental Safety 60: 324-349.

28. Pill WG, Necker AD (2001) The effect of seed treatment on germination and establishment of Kentucky blue grass (Poa pretenses L.). Seed Science and Technology 29: 65-72.

29. Rajasekaran LR, Stiles A, Surette MA, Sturz AV, Blake TJ, Caldwell C, Nowak J (2002) Stand Establishment Technologies for Processing Carrots: Effects of various temperatures regimes on germination and the role of Salicylates in promoting germination at low temperatures. Canadian Journal of Plant Science 82: 443-450.

30. Saxena NP, Johansson C, Saxena MC, Silim, SN (1993) Selection drought and salinity tolerance in cool–season legumes. Plant Cell Environment 16: 524-536.

31. Shakirova FM (2003) Changes in the hormonal status of wheat seedlings induced by SA and salinity. Plant Science 164: 317-322.

32. Sharma AD, Thakur M, Rana M, Singh K (2004) Effect of plant growth hormones and abiotic stresses on germination, growth and phosphates activities in Sorghum bicolor L. Moench seeds. African Journal of Biotechnology 3: 308-312.

33. Shekari F, Balejani R, Saba G, Afsahi K, Shekari F (2008) Priming effect of salicylic acid on seedling growth characteristics of borage (Borago officinalis). Journal of Modern Agriculture 18: 47-53.

34. Wang L, Chen S, Kong W, Li S, Archbold DD (2006) Salicylic acid pretreatment alleviates chilling injury and affects the antioxidant system and heat shock proteins of peaches during cold storage. Postharvest Biology and Technology 41: 244-251.

35. Yagmur M, Kaydan D (2008) Alleviation of osmotic stress of water and salt in germination and seedling growth of triticale with seed priming treatment. African Journal of Biotechnology 7: 2156-2162.­