پاسخ‌های فیزیولوژیک و تغییرات پروتئین حرارتی در سرخارگل تحت تنش خشکی
محورهای موضوعی :
بوم شناسی گیاهان زراعی
کوثر مرادی
1
(گروه باغبانی، دانشکده کشاورزی، واحد خوراسگان، دانشگاه آزاد اسلامی، اصفهان، ایران)
فریبا خلیلی
2
(گروه باغبانی، دانشکده کشاورزی، واحد خوراسگان، دانشگاه آزاد اسلامی، اصفهان، ایران)
تاریخ دریافت : 1397/05/12
تاریخ پذیرش : 1397/10/18
تاریخ انتشار : 1397/10/01
کلید واژه:
تنش خشکی,
پرولین,
تنش آبی,
پروتئین شوک حرارتی,
تنش غیرزیستی,
چکیده مقاله :
سلول‌‌های گیاهی جهت درک سیگنال‌‌های مختلف پیرامون شان نمو یافته‌‌اند و از طریق تعدیل بیان ژن‌‌ها به آن‌‌ها پاسخ می‌‌دهند. خشکی یک ویژگی آب و هوایی طبیعی و محدود کننده عملکرد گیاهان زراعی است. در این پژوهش به منظور ارزیابی واکنش گیاه سرخارگل به تنش خشکی، در یک آزمایش گلدانی، پس از اعمال سطح تنش خشکی شامل آبیاری به میزان 25، 50، 75 و 85% ظرفیت زراعی همراه با آبیاری معمول، میزان پرولین، پتاسیم، فسفر و نیتروژن برگ اندازه گیری و نیز بیان پروتئین های شوک حرارتی با استفاده از روش Real- Time PCR در بافت برگ مورد ارزیابی قرار گرفت. افزایش معنی‌دار میزان پرولین، کاهش پتاسیم، فسفر و نیتروژن در بافت برگ در اثر اعمال تنش خشکی مشاهده شد. همچنین اعمال تنش خشکی موجب افزایش بیان پروتئین شوک حرارتی در تمامی سطوح تنش مورد مطالعه شد. به طور کلی، گیاه سرخارگل با به کارگیری برخی سازوکارهای دفاعی از قبیل افزایش محتوی پرولین و پروتئین شوک حرارتی در مقابل تنش خشکی مقاومت می کند.
چکیده انگلیسی:
Plant cells have evolved to understand the various signals in their surroundings and respond to them by modulating the expression of genes. Drought is a natural and recurrent climatic feature in most parts of the world and plays an important and limiting role in crop yields. In this study, to ensure the stress on the medicinal herb of coneflower (Echinacea purpurea), the proline, potassium, phosphorus and nitrogen content of the leaves were evaluated in a completely randomized design with three replications, each of which was repeated in three pots. Also, expression of heat shock proteins in leaf tissue under four levels of drought stress irrigation at 25%, 50%, 75% and 85% of crop capacity was evaluated. The results showed a significant increase in the amount of proline, potassium, phosphorus and nitrogen in leaf tissue. Also, examination of thermal shock protein expression using Real-Time PCR indicated that drought stress significantly increased expression of heat shock protein in all studied treatments, which also proved the changes caused by stress. In general, the coneflower plant resists some degree of resistance using of some protective mechanisms, such as increasing proline and heat shock proteins content.
منابع و مأخذ:
Anrist Rangel Y (2008) Quantifying mineral source of potassium in agricultural soils. PhD Thesis, Swedish University of Agricultural Sciences, Acta Universitatis Agriculturae Sueciae 53: 105.
Barnes J, Anderson LA, Gibbons S, Phillipson JD (2005) Echinacea species (Echinacea angustifolia (DC.) Hell, Echinacea pallida (Nutt.) Nutt, Echinacea purpurea (L.) Moench): a review of their chemistry, pharmacology and clinical properties. Journal of Pharmacy and Pharmacology 57(8): 929-954.
Bates LS, Waldern RP, Tear ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 39: 205-207.
Duan YH, Guo J, Ding K, Wang SJ, Zhang H, Dai XW (2011) Characterization of a wheat HSP70 gene and its expression in response to stripe rust infection and abiotic stresses. Molecular Biology Reports 38: 301–307.
Ge TD, Sun NB, Bai LP, Tong CL, Sui FG (2012) Effects of drought stress on phosphorus and potassium uptake dynamics in summer maize (Zea mays) throughout the growth cycle. Acta Physiologiae Plantarum 34(6): 2179-2186.
Hald PM (1947) The flame photometer for the measurement of sodium and potassium in biological materials. The Journal of Biological Chemistry 167: 499-510.
Hawkesford M, Horst W, Kichey T, Lambers H, Schjoerring J, Skrumsager Moller I, White P (2012) Functions Of Macronutrients. In: Marschner's Mineral Nutrition of Higher Plants (ed. Marschner, P.). Academic Press: London 135–189.
Kotak S, Larkindale J, Lee U, von Koskull-Döring P, Vierling E, Scharf KD (2007) Complexity of the heat stress response in plants. Current Opinion in Plant Biology 10(3):310-316.
Lack Sh, Naderi A, Siadat SA, Aieneband A, Noormohamadi G (2006) Effect of different levels of nitrogen and plant density on grain yield and its components and water use efficiency of maize (Zea mays L.) cv. SC704 under different moisture conditions in Khuzestan. Journal of Agricultural Science 8:2: 153-170. [in Persian with English abstract]
Matysik J, Alia Bhalu B, Mohanty P (2002) Molecular mechanisms of quenching of reactive oxygen species by proline under stress in plants. Current Science 82: 525-532.
Montero-BarrientosM, Hermosa R, Elena Cardoza R, Monte E (2010) Transgenic expression of the Trichoderma harzianum hsp70 gene increases Arabidopsis resistance to heat and other abiotic stress. Journal of Plant Physiology 167: 659-665.
Mittler R, Zilinskas B (1992) Molecular cloning and characterization of a gene encoding pea cytosolic ascorbate peroxidase. Journal of Biological Chemistry 30: 21802-21807.
Pirzad A, Shakiba MR, Zehtab Salmasi S, Mohamadi SA (2015) Effect of water stress on absorption of some nutrients in Matricaria chamomilla L. Agriculture Journal 106:1-7. [in Persian with English abstract]
Rejeb KB, Abdelly C, Savouré A (2014) How reactive oxygen species and proline face stress together. Plant Physiol Biochem 80:278-284.
Sato Y, Yokoya S (2008) Enhanced tolerance to drought stress in transgenic rice plants overexpressing a small heat-shock protein, sHSP17. 7. Plant Cell Reports 27(2):329-334.
Schmittgen TD, Livak KJ (2008) Analyzing real-time PCR data by the comparative CT method. Nature Protocols 3: 1101-1108.
Sung DY, Vierling E, Guy CL (2001) Comprehensive expression profile analysis of the Arabidopsis Hsp70 gene family. Plant Physiology 126: 789–800.
Wu QS, Xia RX (2006) Arbuscular mycorrhizal fungi influence growth, osmotic adjustment and photosynthesis of citrus under well-watered and water stress conditions. Journal of Plant Physiology 163(4): 417-425.
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Anrist Rangel Y (2008) Quantifying mineral source of potassium in agricultural soils. PhD Thesis, Swedish University of Agricultural Sciences, Acta Universitatis Agriculturae Sueciae 53: 105.
Barnes J, Anderson LA, Gibbons S, Phillipson JD (2005) Echinacea species (Echinacea angustifolia (DC.) Hell, Echinacea pallida (Nutt.) Nutt, Echinacea purpurea (L.) Moench): a review of their chemistry, pharmacology and clinical properties. Journal of Pharmacy and Pharmacology 57(8): 929-954.
Bates LS, Waldern RP, Tear ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 39: 205-207.
Duan YH, Guo J, Ding K, Wang SJ, Zhang H, Dai XW (2011) Characterization of a wheat HSP70 gene and its expression in response to stripe rust infection and abiotic stresses. Molecular Biology Reports 38: 301–307.
Ge TD, Sun NB, Bai LP, Tong CL, Sui FG (2012) Effects of drought stress on phosphorus and potassium uptake dynamics in summer maize (Zea mays) throughout the growth cycle. Acta Physiologiae Plantarum 34(6): 2179-2186.
Hald PM (1947) The flame photometer for the measurement of sodium and potassium in biological materials. The Journal of Biological Chemistry 167: 499-510.
Hawkesford M, Horst W, Kichey T, Lambers H, Schjoerring J, Skrumsager Moller I, White P (2012) Functions Of Macronutrients. In: Marschner's Mineral Nutrition of Higher Plants (ed. Marschner, P.). Academic Press: London 135–189.
Kotak S, Larkindale J, Lee U, von Koskull-Döring P, Vierling E, Scharf KD (2007) Complexity of the heat stress response in plants. Current Opinion in Plant Biology 10(3):310-316.
Lack Sh, Naderi A, Siadat SA, Aieneband A, Noormohamadi G (2006) Effect of different levels of nitrogen and plant density on grain yield and its components and water use efficiency of maize (Zea mays L.) cv. SC704 under different moisture conditions in Khuzestan. Journal of Agricultural Science 8:2: 153-170. [in Persian with English abstract]
Matysik J, Alia Bhalu B, Mohanty P (2002) Molecular mechanisms of quenching of reactive oxygen species by proline under stress in plants. Current Science 82: 525-532.
Montero-BarrientosM, Hermosa R, Elena Cardoza R, Monte E (2010) Transgenic expression of the Trichoderma harzianum hsp70 gene increases Arabidopsis resistance to heat and other abiotic stress. Journal of Plant Physiology 167: 659-665.
Mittler R, Zilinskas B (1992) Molecular cloning and characterization of a gene encoding pea cytosolic ascorbate peroxidase. Journal of Biological Chemistry 30: 21802-21807.
Pirzad A, Shakiba MR, Zehtab Salmasi S, Mohamadi SA (2015) Effect of water stress on absorption of some nutrients in Matricaria chamomilla L. Agriculture Journal 106:1-7. [in Persian with English abstract]
Rejeb KB, Abdelly C, Savouré A (2014) How reactive oxygen species and proline face stress together. Plant Physiol Biochem 80:278-284.
Sato Y, Yokoya S (2008) Enhanced tolerance to drought stress in transgenic rice plants overexpressing a small heat-shock protein, sHSP17. 7. Plant Cell Reports 27(2):329-334.
Schmittgen TD, Livak KJ (2008) Analyzing real-time PCR data by the comparative CT method. Nature Protocols 3: 1101-1108.
Sung DY, Vierling E, Guy CL (2001) Comprehensive expression profile analysis of the Arabidopsis Hsp70 gene family. Plant Physiology 126: 789–800.
Wu QS, Xia RX (2006) Arbuscular mycorrhizal fungi influence growth, osmotic adjustment and photosynthesis of citrus under well-watered and water stress conditions. Journal of Plant Physiology 163(4): 417-425.
