مقایسه تاثیر تمرینات مقاومتی، استقامتی و ترکیبی بر بیان ژن رزیستین دربافت‌های ماهیچه دست و پای موش های نر نژاد ویستار
محورهای موضوعی : نشانگر های زیستی پلاسمافاطمه حاجی قربانی زاده 1 , طاهره باقرپور 2 , نعمت اله نعمتی 3
1 - دانشجو ی دکتری فیزیولوژی ورزشی ، گروه تربیت بدنی ، واحد دامغان ، دانشگاه آزاد اسلامی ، دامغان ، ایران.
2 - استادیار گروه تربیت بدنی ، واحد دامغان ، دانشگاه آزاد اسلامی ، دامغان ، ایران
3 - دانشیار گروه تربیت بدنی ، واحد دامغان ، دانشگاه آزاد اسلامی ، دامغان ، ایران.
کلید واژه: رزیستین, تمرین ترکیبی, تمرین استقامتی , تمرین مقاومتی,
چکیده مقاله :
زمینه و هدف: رزیستین آدیپوکاینی است که توسط بافت‌های مختلف بدن ترشح می‌شود. این آدیپوکاین نقش مهمی در متابولیسم قند و لیپیدها ایفا می‌کند. مطالعه حاضر با هدف بررسی تأثیر تمرینات استقامتی، مقاومتی و ترکیبی بر سطح بیان ژن آدیپونکاین در ماهیچه‌های دست و پای موش‌های صحرایی نر نژاد ویستار انجام شد.
مواد و روش‌ها: در این مطالعه تجربی، 40 سر موش صحرایی نر سالم نژاد ویستار به چهار گروه ده‌تایی کنترل، تمرین مقاومتی، تمرین استقامتی و ترکیبی تقسیم شدند. هر گروه به مدت 8 هفته تمرین‌های مختص خود را انجام دادند. در نهایت، ماهیچه‌های دست و پا برای اندازه‌گیری بیان ژن رزیستین مورد بررسی قرار گرفتند. برای مقایسه گروه‌ها با هم، از آزمون تحلیل واریانس یک طرفه و برای مقایسه درون گروهی از آزمون تی زوجی استفاده شد.
نتایج: این مطالعه نشان داد که تمرینات ورزشی مختلف می‌توانند سطح بیان ژن رزیستین را در عضلات دست و پا نسبت به گروه کنترل افزایش دهند (001/0>P). همچنین، گروه‌های مورد مداخله در مقایسه با یکدیگر سطوح مختلفی از ژن رزیستین را بیان کردند، به طوری که تمرین ترکیبی بیشترین تاثیر را در کاهش بیان ژن رزیستین در مقایسه با تمرین‌های مقاومتی و استقامتی در عضله کند انقباض پا داشت (001/0>P).
نتیجه گیری: تمرین ورزشی ترکیبی، تمرین مقاومتی و تمرین استقامتی می‌توانند سطح بیان ژن رزیستین را در عضلات دست و پا نسبت به گروه کنترل کاهش دهند.
Background & Aim: Resistin is an adipokine secreted by adipocytes, but it can also be produced by other organs. This adipokine is crucial for glucose and lipid metabolism. The study aimed to explore the impact of endurance, resistance, and combined exercises on adiponkine gene expression in the hand and leg muscles of male Wistar rats.
Materials & Methods: In this experimental study, 40 healthy male Wistar rats were divided into four groups: control, resistance training, endurance training, and combined. Each group conducted their respective exercises for 8 weeks. Subsequently, hand and foot muscles were analyzed to measure resistin gene expression. The groups were compared using a one-way analysis of variance test, and intra-group comparisons were made using paired t-tests.
Results: The study's findings indicate that different sports exercises can elevate the expression of the resistin gene in hand and foot muscles compared to the control group (P <0.001). Furthermore, the intervention groups exhibited varying levels of resistin gene expression relative to each other, with concurrent training (0.02 ± 0.12) having the most significant impact on resistin gene expression compared to resistance training (0.04 ± 0.21) and endurance (0.04 ± 0.50) in slow twitch muscle of leg (P<0.001).
Conclusion: Concurrent trainings, resistance training, and endurance training can mitigate the expression level of the resistin compared to the control group.
فهرست منابع
1. Steppan CM, Bailey ST, Bhat S, Brown EJ, Banerjee RR, Wright CM, et al. The hormone resistin links obesity to diabetes. Nature. 2001;409(6818):307-12.
2. Perseghin G, Burska A, Lattuada G, Alberti G, Costantino F, Ragogna F, et al. Increased serum resistin in elite endurance athletes with high insulin sensitivity. Diabetologia. 2006;49:1893-900.
3. Kim K-H, Lee K, Moon YS, Sul HS. A cysteine-rich adipose tissue-specific secretory factor inhibits adipocyte differentiation. Journal of Biological Chemistry. 2001;276(14):11252-6.
4. Muse ED, Obici S, Bhanot S, Monia BP, McKay RA, Rajala MW, et al. Role of resistin in diet-induced hepatic insulin resistance. The Journal of clinical investigation. 2004;114(2):232-9.
5. Ouchi N, Parker JL, Lugus JJ, Walsh K. Adipokines in inflammation and metabolic disease. Nature reviews immunology. 2011;11(2):85-97.
6. McTernan PG, Kusminski CM, Kumar S. Resistin. Current opinion in lipidology. 2006;17(2):170-5.
7. Nogueiras R, Gallego R, Gualillo O, Caminos JE, García-Caballero T, Casanueva FF, et al. Resistin is expressed in different rat tissues and is regulated in a tissue- and gender-specific manner. FEBS letters. 2003;548(1-3):21-7.
8. Codoñer-Franch P, Alonso-Iglesias E. Resistin: insulin resistance to malignancy. Clinica chimica acta; international journal of clinical chemistry. 2015;438:46-54.
9. Kusminski CM, McTernan PG, Kumar S. Role of resistin in obesity, insulin resistance and Type II diabetes. Clinical science (London, England : 1979). 2005;109(3):243-56.
10. Askin L, Abus S, Tanriverdi O. Resistin and Cardiovascular Disease: A Review of the Current Literature Regarding Clinical and Pathological Relationships. Current cardiology reviews. 2022;18(1):e290721195114.
11. Nagaev I, Bokarewa M, Tarkowski A, Smith U. Human resistin is a systemic immune-derived proinflammatory cytokine targeting both leukocytes and adipocytes. PloS one. 2006;1(1):e31.
12. Krysiak R, Handzlik-Orlik G, Okopien B. The role of adipokines in connective tissue diseases. European journal of nutrition. 2012;51:513-28.
13. Hashemi N, Mirfeyzi SZ, Sahebari M, Rezaeeyazdi Z. Adipokines: resistin and systemic rheumatic diseases. medical journal of mashhad university of medical sciences. 2015;58(8):473-80.
14. Acquarone E, Monacelli F, Borghi R, Nencioni A, Odetti P. Resistin: A reappraisal. Mechanisms of ageing and development. 2019;178:46-63.
15. Huang X, Yang Z. Resistin’s, obesity and insulin resistance: the continuing disconnect between rodents and humans. Journal of endocrinological investigation. 2016;39:607-15.
16. Fargnoli JL, Fung TT, Olenczuk DM, Chamberland JP, Hu FB, Mantzoros CS. Adherence to healthy eating patterns is associated with higher circulating total and high-molecular-weight adiponectin and lower resistin concentrations in women from the Nurses' Health Study. The American journal of clinical nutrition. 2008;88(5):1213-24.
17. Marcelino-Rodríguez I, Almeida Gonzalez D, Alemán-Sánchez JJ, Brito Díaz B, Rodríguez Pérez MDC, Gannar F, et al. Inverse association of resistin with physical activity in the general population. PLoS One. 2017;12(8):e0182493.
18. Saeidi A, Haghighi MM, Kolahdouzi S, Daraei A, Abderrahmane AB, Essop MF, et al. The effects of physical activity on adipokines in individuals with overweight/obesity across the lifespan: A narrative review. Obesity reviews. 2021;22(1):e13090.
19. Sirico F, Bianco A, D'Alicandro G, Castaldo C, Montagnani S, Spera R, et al. Effects of physical exercise on adiponectin, leptin, and inflammatory markers in childhood obesity: systematic review and meta-analysis. Childhood Obesity. 2018;14(4):207-17.
20. Khalafi M, Sakhaei MH, Kheradmand S, Symonds ME, Rosenkranz SK. The impact of exercise and dietary interventions on circulating leptin and adiponectin in individuals with overweight and obesity: a systematic review and meta-analysis. Advances in Nutrition. 2022.
21. Jadhav RA, Maiya GA, Hombali A, Umakanth S, Shivashankar K. Effect of physical activity promotion on adiponectin, leptin and other inflammatory markers in prediabetes: a systematic review and meta-analysis of randomized controlled trials. Acta diabetologica. 2021;58:419-29.
22. Khalafi M, Sakhaei MH, Kheradmand S, Symonds ME, Rosenkranz SK. The impact of exercise and dietary interventions on circulating leptin and adiponectin in individuals who are overweight and those with obesity: A systematic review and meta-analysis. Advances in Nutrition. 2023;14(1):128.
23. Rajala MW, Qi Y, Patel HR, Takahashi N, Banerjee R, Pajvani UB, et al. Regulation of resistin expression and circulating levels in obesity, diabetes, and fasting. Diabetes. 2004;53(7):1671-9.
24. Simpson KA, Singh MAF. Effects of exercise on adiponectin: a systematic review. Obesity. 2008;16(2):241-56.
25. Afshounpour MT, Habibi A, Ranjbar R. Impact of combined exercise training on plasma concentration of Apelin, resistin and insulin resistance in patients with type 2 diabetics’ male. HMJ. 2016;20(3):158-69.
26. Shafiee Z, Sharifi G. Comparing the Effect of Resistance, Aerobic, and Concurrent Exercise Program on the Level of Resistin and High Reactive Protein C of Overweight and Obese Women. International Archives of Health Sciences. 2017;4(1):1-6.
27. AfshounPour M, Davoodi Z, Habibi H, Ranjbar R, Shakerian S. The effect of circuit resistance exercise on plasma resistin concentration and insulin resistance in type 2 diabetic men. SSU_Journals. 2015;23(8):770-81.
28. Abedi B, Karimi M, Mehranpour A, Sayyah M. Effects of eight-weeks of aerobic training on resistin levels and insulin resistance in sedentary middle-aged women. Journal of Kermanshah University of Medical Sciences. 2017;21(3).
29. Shokri E, Heidarianpour A, Razavi Z. Positive effect of combined exercise on adipokines levels and pubertal signs in overweight and obese girls with central precocious puberty. Lipids in Health and Disease. 2021;20(1):1-14.
30. Ihalainen JK, Schumann M, Eklund D, Hämäläinen M, Moilanen E, Paulsen G, et al. Combined aerobic and resistance training decreases inflammation markers in healthy men. Scandinavian journal of medicine & science in sports. 2018;28(1):40-7.
31. Jiménez-Martínez P, Ramirez-Campillo R, Alix-Fages C, Gene-Morales J, García-Ramos A, Colado JC, editors. Chronic Resistance Training Effects on Serum Adipokines in Type 2 Diabetes Mellitus: A Systematic Review. Healthcare; 2023: MDPI.
32. Pedersen BK. Anti‐inflammatory effects of exercise: role in diabetes and cardiovascular disease. European journal of clinical investigation. 2017;47(8):600-11.
33. Nimmo M, Leggate M, Viana J, King J. The effect of physical activity on mediators of inflammation. Diabetes, Obesity and Metabolism. 2013;15(s3):51-60.
34. García-Hermoso A, Ramírez-Vélez R, Díez J, González A, Izquierdo M. Exercise training-induced changes in exerkine concentrations may be relevant to the metabolic control of type 2 diabetes mellitus patients: A systematic review and meta-analysis of randomized controlled trials. Journal of Sport and Health Science. 2023;12(2):147-57.
35. Golbidi S, Laher I. Exercise induced adipokine changes and the metabolic syndrome. Journal of diabetes research. 2014;2014.
36. Abdi A. The effect of aerobic, resistance, and concurrent training on the expression and protein levels of RBP4 visceral and subcutaneous adipose tissue in diabetic rats with STZ. International Journal of Diabetes in Developing Countries. 2023;43(2):309-16.
37. Jamali E, Asad MR, Rassouli A. Effect of eight-week endurance exercise on resistin gene expression in visceral adipose tissues in obese rats. SSU_Journals. 2017;25(1):20-31.
38. BULDUK B, GÜNBATAR N. The Effect of Exercise on Serum Resistin and Leptin Values in Rats Fed with a High Fat Diet. Journal of Contemporary Medicine. 2023;13(1):82-5.
39. Mohammad P, Esfandiar KZ, Abbas S, Ahoora R. Effects of moderate-intensity continuous training and high-intensity interval training on serum levels of resistin, chemerin and liver enzymes in streptozotocin-nicotinamide induced type-2 diabetic rats. Journal of diabetes & metabolic disorders. 2019;18:379-87.
40. Jamali E, Asad MR, Rassouli A. The effect of high-intensity interval training (HIIT) on resistin gene expression in visceral adipose tissue in obese male rats. International Journal of Applied Exercise Physiology. 2016;5(1).
41. Hosseini M, Bagheri Afsariehee MR. The effect of high intensity interval training and aloe Vera consumption on resistin and insulin resistance index in diabetic rat. KAUMS Journal (FEYZ). 2018;22(4):370-8.
42. Varady KA, Bhutani S, Church EC, Phillips SA. Adipokine responses to acute resistance exercise in trained and untrained men. Medicine and science in sports and exercise. 2010;42(3):456-62.
43. Rashidlamir A, Gholamian S, Ebrahimi Atri A, Seyyedalhoseyni M, Hesar Kooshki M. Effect of regular aerobic exercise on plasma levels of resistin and adiponectin in active young females. Journal of Mazandaran University of Medical Sciences. 2013;23(101):67-76.
44. Jafari Ghaleh No SA, Fathi M, Hejazi K, Ziayi M. The effects of eight weeks of aerobic interval exercise on omentin-1, resistin, and adiponectin in elderly men with type 2 diabetes. Pathobiology Research. 2017;20(3):17-32.
45. Baskin Kedryn K, Winders Benjamin R, Olson Eric N. Muscle as a “Mediator” of Systemic Metabolism. Cell Metabolism. 2015;21(2):237-48.
