اثرات مکمل روی-متیونین بر شاخص های آنتی اکسیدانی و آنزیم های کبدی در پلاسمای خون اسب فریزین در شرایط تنش گرمایی
محورهای موضوعی : زیست شناسی جانوریاحمد میری 1 , علی اصغر صادقی 2 , محمد چمنی 3 , آسا ابراهیمی 4
1 - دانشجوی دوره دکتری، گروه علوم دامی، واحد علوم و تحقيقات، دانشگاه آزاد اسلامي، تهران، ايران
2 - دانشیار، گروه علوم دامی، واحد علوم و تحقيقات، دانشگاه آزاد اسلامي، تهران، ايران
3 - استاد، گروه علوم دامی، واحد علوم و تحقيقات، دانشگاه آزاد اسلامي، تهران، ايران
4 - دانشیار، گروه بیوتکنولوژی و به نژادی، واحد علوم و تحقيقات، دانشگاه آزاد اسلامي، تهران، ايران
کلید واژه: اسب, مکمل روی-متیونین, آنزیم های آنتی اکسیدانی, آنزیم های کبدی, تنش گرمایی,
چکیده مقاله :
هدف: هدف از انجام پژوهش حاضر مطالعه اثرات افزودن مکمل روی-متیونین به جیره اسب در معرض تنش گرمایی بر شاخص های آنتی اکسیدانی خون، فعالیت آنزیم های آنتی اکسیدانی و فعالیت آنزیم های کبدی بود. مواد و روش ها: در قالب آزمون فاکتوریل بر پایه طرح کاملا تصادفی، 30 راس اسب فریزین در دو سطح تنش گرمایی و سه سطح مکمل روی-متیونین اعمال شد. سطوح تنش شامل 1) بدون تنش و 2) با تنش گرمایی بود. سه سطح مکمل روی-متیونین استفاده شد شامل 1) بدون مکمل، 2) مقدار 67 میلی گرم روی-متیونین در کیلوگرم جیره و 3) مقدار 120 میلی گرم روی-متیونین در کیلوگرم جیره. از اسب ها نمونه خون تهیه شد و شاخص های آنتی اکسیدانی و فعالیت آنزیم های کبدی اندازه گیری شد. نتایج: تنش گرمایی سبب افزایش معنی دار غلظت مالون دی آلدئید و افزودن مکمل روی-متیونین سبب کاهش معنی دار غلظت این ماده در خون شد. افزایش دوز روی-متیونین تغییر معنی داری بر غلظت مالون دی آلدئید نداشت. اثر متقابل تنش و مکمل بر فعالیت آنزیم سوپراکسید دیسموتاز تمایل به معنی داری و بر فعالیت آنزیم گلوتاتیون پراکسیداز معنی دار بود. فعالیت آنزیم های آلانین آمینوترانسفراز، آسپارتات آمینوترانسفراز و آلکالین فسفاتاز تحت تاثیر تنش گرمایی افزایش و با افزودن مکمل روی-متیونین کاهش معنی داری نشان داد. نتیجه گیری: استفاده از مکمل روی-متیونین در دوز 67 میلی گرم در کیلوگرم می تواند اثرات منفی تنش گرمایی بر شاخص های آنتی اکسیدانی و آنزیم های کبدی را در اسب های فریزین مرتفع کند.
Aim: The purpose of this study was to evaluate the effects of dietary zinc-methionine supplementation in horses exposed to heat stress on blood antioxidant indices, antioxidant enzyme activity and liver enzyme activity. Materials and Methods: In a factorial trial based on a completely randomized design, 30 Frisian horses were applied in two levels of heat stress and three levels of zinc-methionine supplementation. Stress levels included; 1) no stress; 2) exposed to heat stress. Three levels of zinc-methionine supplementation were used, including; 1) no supplementation, 2) 67 mg of zinc-methionine per kg of diet and 3) 120 mg of zinc-methionine per kg of diet. Blood samples were taken from horses and antioxidant indices and liver enzyme activity were measured. Results: Heat stress increased and zinc-methionine supplementation decreased significantly the concentration of malondialdehyde. An increase in the dose of zinc-methionine had no significant effect on the concentration of malondialdehyde. The interaction effect of stress and supplementation on the activity of superoxide dismutase enzyme tended to be significant and also on the activity of glutathione peroxidase enzyme. The activity of alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase enzymes increased in horses exposed to heat stress and showed a significant decrease with zinc-methionine supplementation. Conclusion: The zinc-methionine supplementation at a dose of 67 mg/kg can eliminate the negative effects of heat stress on antioxidant indices and liver enzymes in Friesian horses.
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