بررسی اثر پروتئین فعال کننده نوتروفیلی هلیکوباکتر پیلوری بر میزان لکوترینهایC4, B4 و التهاب مجاری هوایی در موش مدل آسم آلرژیک
اثر پروتئین فعال کننده نوتروفیلی هلیکوباکتر بررC4, B4 و التهاب آسم
محورهای موضوعی : نشانگر های زیستی پلاسما
علیرضا خالقی خرمی
1
,
رسول شکری
2
,
Seyyed Shamsadin Athari
3
,
ساناز مهمازی
4
1 -
2 - مرکز تحقیقات زیست شناسی، واحد زنجان، دانشگاه آزاد اسلامی، زنجان، ایران
3 - Department of Immunology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
4 - دانشکده علوم پایه و فنی و مهندسی، دانشگاه آزاد اسلامی واحد زنجان، زنجان ، ایران.
کلید واژه: هلیکوباکتر پیلوری, آسم آلرژیک, پروتئین فعال کننده نوتروفیلی, لکوترین,
چکیده مقاله :
پیش زمینه وهدف پروتئین فعال کننده نوتروفیلی هلیکوباکتر پیلوری (HP-NAP) به عنوان یک ایمونوژن برای برانگیختن پاسخ های آنتی ژنی یا یک تعدیل کننده ایمنی برای تنظیم ایمنی سلول های T عمل می کند. این پروتئین می تواند یک پاسخ Th2 منجر به پاسخ آلرژیک را به سمت پاسخ Th1 سوق دهد .همچنین احتمال می رود بر میزان تولید لکوترینهای C4, B4 و نهایتا بیماری آسم نیز تاثیر بگذارد. هدف از این مطالعه بررسی تاثیر HP-NAP بر میزان لکوترینهای C4, B4 در موش های مدل آسم آلرژیک و مقایسه آن با تاثیرات خود هلیکو باکترپیلوری (Helicobacter pylori) می باشد. مواد وروشها در ابتدا سویه استاندارد هلیکوباکتر پیلوری و پپتید HP-NAP طبق مطالعه قبلی تهیه شد. سپس 40 سر موش نر بالغ Blab c 6 تا 8 هفته‌ای برای مدل‌سازی و ایجاد آسم آلرژیک تهیه و به 4 گروه 10 تایی تقسیم و برای مطالعه تغییرات لکوترینهای مذکورانتخاب شدند. نتایج در اندازه گیری سطح لکوترینهای C4, B4 ،با استفاده از روش الایزا مشاهده شد که سطح C4, B4 گروه آسمی به طور معناداری نسبت به گروه سالم افزایش دارد. در حالی که تیمارها با هلیکوباکتر و HP-NAP منجر به کاهش معنی‌داری در سطوح C4, B4 نسبت به گروه آسمی نشد. نتیجه گیری با توجه به اثرات قوی تر HP-NAP نسبت به خود هلیکو باکتر در کاهش سطح لکوترینهای C4, B4 ، HP-NAP توانست در حیوانات آسمی میزان لکوترینهای C4, B4را کاهش دهد ولی میزان کاهش آن نسبت به گروه آسمی معنی دار نبود .بنابراین مطالعات بیشتر پیشنهاد می گردد.
Background & Aim: Helicobacter pylori neutrophil-activating protein (HP-NAP) acts as an immunogen to elicit antigenic responses or an immunomodulator to regulate T-cell immunity. This protein can lead to a Th2 response, which may result in an allergic response or a Th1 response. It is also likely to affect the production of leukotrienes C4, B4 and finally asthma. The objective of this study is to examine the impact of HP-NAP on the concentration of leukotrienes C4 and B4 in an allergic asthma model in mice and to compare this with the effects of Helicobacter pylori. Materials & Methods: Firstly, the Helicobacter pylori standard strain and HP-NAP peptide were prepared in accordance with the methodology outlined in a previous study. Subsequently, 40 adult Blab c 6 to 8-week-old male mice were prepared for modelling and developing allergic asthma and were divided into four groups of 10. These mice were selected for further study to investigate the changes in the aforementioned leukotrienes. Results: demonstrated that the level of leukotrienes C4 and B4, as measured using the ELISA method, was significantly elevated in the asthmatic group compared to the healthy group. While the treatments with Helicobacter and HP-NAP did not result in a significant decrease in the levels of C4 and B4 compared to the asthmatic group, Conclusion: In light of the more pronounced effects of HP-NAP compared to Helicobacter itself in reducing the level of C4 and B4 leukotrienes, HP-NAP was able to reduce the level of C4 and B4 leukotrienes in asthmatic animals. However, this reduction was not statistically significant when compared to the asthmatic group. Therefore, further studies are recommended.
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