Preconcentration and Determination of Theophylline in Water Samples using Magnetic Nano-Cellulose with Dispersive Solid Phase Extraction
محورهای موضوعی : شیمی داروئی
Parastoo Molaei
1
(Faculty of Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran)
ali moghimi
2
(Department of chemistry, Varamin Pishva Branch, Islamis Azad University, Varamin, Iran)
Shahin Ahmadi
3
( Faculty of Medicinal Chemistry, Department of Chemistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran)
seyedali sobhanian
4
(Faculty of Pharmaceutical Sciences, Department of Medicinal Chemistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran)
کلید واژه: Theophylline, Dispersive Solid Phase Extraction, Preconcentration, Measurement, Magnetic nanoparticles, Nanocellulose.,
چکیده مقاله :
Theophylline (1,3-dimethyl-2,3,6,7-tetrahydro-1-hydrogen-purine-6,2-dione) is a bronchodilator drug used in the treatment of asthma and chronic obstructive pulmonary disease (COPD). Due to the narrow therapeutic strip of this drug, it is important to measure its blood level.To achieve a simple, safe and sensitive method based on dispersive solid phase extraction (DSPE) using magnetite nanoparticles covered with nanocellulose and beta-cyclodextrin for preconcentration and measurement of theophylline in aqueous samples and its validation, this method was used. In this research, variables affecting the extraction such as pH, ionic strength, and amount of adsorbent, temperature, time, limit volume and type of solvent were optimized. After extraction, the adsorbent was easily separated from the aqueous sample using an external magnetic field without filtration or centrifugation. The results showed that optimum conditions were obtained with pH=5, 1 mg of sodium chloride, 0.2 g of adsorbent with a capacity of 0.53 mg/g, temperature of 40 degrees Celsius, 20 minutes of contact between the adsorbent and the sample and 5 mL of methanol as the desorption solvent. In optimal conditions, the suitable concentration factor is 12, low detection limit (0.17 mg/L), wide linear range and in accordance with the therapeutic strip of the drug (5-50 mg/L), good correlation coefficient (R2=0.9991) and good accuracy (RSD=1.03%) was obtained.Urine sample was used to validate the above method. Also, it was found that the proposed method is suitable, efficient and usable for measuring of theophylline.
Theophylline (1,3-dimethyl-2,3,6,7-tetrahydro-1-hydrogen-purine-6,2-dione) is a bronchodilator drug used in the treatment of asthma and chronic obstructive pulmonary disease (COPD). Due to the narrow therapeutic strip of this drug, it is important to measure its blood level.To achieve a simple, safe and sensitive method based on dispersive solid phase extraction (DSPE) using magnetite nanoparticles covered with nanocellulose and beta-cyclodextrin for preconcentration and measurement of theophylline in aqueous samples and its validation, this method was used. In this research, variables affecting the extraction such as pH, ionic strength, and amount of adsorbent, temperature, time, limit volume and type of solvent were optimized. After extraction, the adsorbent was easily separated from the aqueous sample using an external magnetic field without filtration or centrifugation. The results showed that optimum conditions were obtained with pH=5, 1 mg of sodium chloride, 0.2 g of adsorbent with a capacity of 0.53 mg/g, temperature of 40 degrees Celsius, 20 minutes of contact between the adsorbent and the sample and 5 mL of methanol as the desorption solvent. In optimal conditions, the suitable concentration factor is 12, low detection limit (0.17 mg/L), wide linear range and in accordance with the therapeutic strip of the drug (5-50 mg/L), good correlation coefficient (R2=0.9991) and good accuracy (RSD=1.03%) was obtained.Urine sample was used to validate the above method. Also, it was found that the proposed method is suitable, efficient and usable for measuring of theophylline.
1. Charehsaz M, Gürbay A, Aydin A, Sahin G. Simple, fast and reliable liquid chromatographic and spectrophotometric methods for the determination of theophylline in urine, saliva and plasma samples. Iran J Pharm Res. 2014 Spring;13(2):431–9.
2. Kossel A. Über eine neue Base aus dem Pflanzenreich. Berichte der deutschen chemischen Gesellschaft. 1888;21(1):2164–7.
3. Katzung B-H, Marieke, Tuan R, Lalchandani, Vanderah TW, Anthony J. Katzung & Trevor’s Pharmacology Examination And Board Review. 2019.
4. Katzung B, Anthony J. Basic & Clinical Pharmacology. Tehran: Andishe Rafi. 2015.
5. Macwan A, Marvaniya V, Patani P. Review on Various Analytical Method for Quantitative Determination of Etophylline and Theophylline in Bulk and in Different Dosage Forms. Asian Journal of Pharmaceutical Technology & Innovation. 2018;06(28):1–5.
6. Al-Jenoobi FI, Ahad A, Mahrous GM, Raish M, Alam MA, Al-Mohizea AM. A simple HPLC-UV method for the quantification of theophylline in rabbit plasma and its pharmacokinetic application. J Chromatogr Sci [Internet]. 2015;53(10):1765–70.
7. Jalal J, Raed H, Mohammad H. Solid Phase Extraction of Theophylline in Aqueous Solutions by Modified Magnetic Iron Oxide Nanoparticles as an Extractor Material and Spectrophotometry Technique for the Determination. Res J Chem Environ. 2019;1(23):94–100.
8. Li G, Wang X, Row KH. Magnetic solid-phase extraction with Fe₃O₄/molecularly imprinted polymers modified by deep eutectic solvents and ionic liquids for the rapid purification of alkaloid isomers (theobromine and theophylline) from green tea. Molecules [Internet]. 2017;22(7).
9. Yisak H, Redi-Abshiro M, Chandravanshi BS. New fluorescence spectroscopic method for the simultaneous determination of alkaloids in aqueous extract of green coffee beans. Chem Cent J [Internet]. 2018;12(1).
10. Abdolmohammad-Zadeh H, Talleb Z. Magnetic solid phase extraction of gemfibrozil from human serum and pharmaceutical wastewater samples utilizing a β-cyclodextrin grafted graphene oxide-magnetite nano-hybrid. Talanta [Internet]. 2015;134:387–93.
11. Sereshti H, Khosraviani M, Samadi S, Amini-Fazl MS. Simultaneous determination of theophylline, theobromine and caffeine in different tea beverages by graphene-oxide based ultrasonic-assisted dispersive micro solid-phase extraction combined with HPLC-UV. RSC Adv [Internet]. 2014;4(87):47114–20.
12. Maranata GJ, Surya NO, Hasanah AN. Optimising factors affecting solid phase extraction performances of molecular imprinted polymer as recent sample preparation technique. Heliyon [Internet]. 2021;7(1):e05934.
13. Martin-Esteban A. Molecularly imprinted polymers: new molecular recognition materials for selective solid-phase extraction of organic compounds. Fresenius J Anal Chem [Internet]. 2001;370(7):795–802.
14. Mullett WM, Lai EPC. Molecularly imprinted solid phase extraction micro-column with differential pulsed elution for theophylline determination. Microchem J [Internet]. 1999;61(2):143–55.
