Synthesis of Silica Nanoparticles Coated by Poly Dopamine Imprinted Polymer for the Determination of Testosterone in Capsules
Payam Tabar
1
(
Department of Chemical Engineering, Damghan Branch, Islamic Azad University, Damghan, Iran
)
Hamid Hashemi-Moghaddam
2
(
Department of Chemistry, Damghan Branch, Islamic Azad University, Damghan, Iran
)
Homa Baghaei
3
(
Department of Food Science and Technology, Damghan Branch, Islamic Azad University, Damghan, Iran
)
کلید واژه: Molecular imprint polymer, Solid-phase extraction, Testosterone, Polydopamine,
چکیده مقاله :
This study outlines the synthesis and initial evaluation of poly dopamine molecularly imprinted polymers (MIPs) designed for the selective extraction of testosterone. The resulting polymer was utilized as a solid-phase extraction (SPE) sorbent material. The molecularly imprinted polymer was created using a reaction mixture that included silica, the template (testosterone), a functional monomer (dopamine), and water as the solvent. Core-shell structure MIP beads were formed through precipitation and oxidative polymerization. During the polymerization process, a complex was formed between the template and the functional monomer, resulting in a three-dimensional polymer network that encapsulated the template molecules upon completion of polymerization. Subsequently, the template molecules were removed through washing. A control polymer, which was a non-imprinted polymer, was synthesized under similar conditions without the template to facilitate a comparative analysis of performance. Characterization of the synthesized polymer was conducted using Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), and Thermogravimetric Analysis (TGA). Optimization of parameters such as pH, extraction time, and adsorption capacity was performed. Ultimately, the concentration of testosterone in urine samples was quantified using UV-Vis spectrophotometry following preconcentration with the synthesized MIP. The results obtained demonstrate the effectiveness of the synthesized polymer in extracting testosterone from real samples.
چکیده انگلیسی :
This study outlines the synthesis and initial evaluation of poly dopamine molecularly imprinted polymers (MIPs) designed for the selective extraction of testosterone. The resulting polymer was utilized as a solid-phase extraction (SPE) sorbent material. The molecularly imprinted polymer was created using a reaction mixture that included silica, the template (testosterone), a functional monomer (dopamine), and water as the solvent. Core-shell structure MIP beads were formed through precipitation and oxidative polymerization. During the polymerization process, a complex was formed between the template and the functional monomer, resulting in a three-dimensional polymer network that encapsulated the template molecules upon completion of polymerization. Subsequently, the template molecules were removed through washing. A control polymer, which was a non-imprinted polymer, was synthesized under similar conditions without the template to facilitate a comparative analysis of performance. Characterization of the synthesized polymer was conducted using Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), and Thermogravimetric Analysis (TGA). Optimization of parameters such as pH, extraction time, and adsorption capacity was performed. Ultimately, the concentration of testosterone in urine samples was quantified using UV-Vis spectrophotometry following preconcentration with the synthesized MIP. The results obtained demonstrate the effectiveness of the synthesized polymer in extracting testosterone from real samples.
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