بررسی اثرات ترمیمی رزمارینیک اسید بر نقیصه تجربی نخاع در مدل حیوانی موش صحرایی نژاد ویستار
محورهای موضوعی : زیست شناسی سلولی تکوینی گیاهی و جانوری ، تکوین و تمایز ، زیست شناسی میکروارگانیسم
سید احمد عمادی
1
,
هادی نداف
2
,
الهام حویزی
3
1 - دانشجوی دوره دستیاری تخصصی جراحی دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران
2 - بخش جراحی، گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران
3 - گروه زیست شناسی، دانشکده علوم، دانشگاه شهید چمران اهواز، اهواز، ایران
کلید واژه: ضایعه نخاعی, رزمارینیک اسید, محافظت عصبی, التهاب, مدل حیوانی,
چکیده مقاله :
سابقه و اهداف: آسیب طناب نخاعی وضعیتی است که در آن پارانشیم نخاع در اثر ضربه یا بیماری های مختلف آسیب می بیند. بخشی از آسیب¬های نخاعی عمدتاً توسط واکنش¬های التهابی ایجاد می¬شوند که عملکرد سلول را تغییر می¬دهد و باعث مرگ سلول¬های عصبی باقی مانده می¬¬شود. رزمارینیک اسید، یک پلی فنول فیتوشیمیایی محلول در آب، دارای خواص آنتی اکسیدانی، ضد التهابی و ضد آپوپتوز است. روش بررسی: در این پژوهش 12 موش صحرایی از نژاد ویستار در دو گروه با جمعیت مساوی مورد مطالعه قرار گرفته اند. گروه کنترل هیچ درمانی پس از آسیب نخاعی تا روز 56 مطالعه دریافت نکردند و گروه رزمارینیک اسید روزانه 100 میلی¬گرم بر کیلوگرم از عصاره رزمارینیک اسید در آب آشامیدنی تا روز 56 پس از آسیب نخاعی دریافت کردند. در طول این دوره موش ها ازنظر حرکتی با آزمون باسو-بتی-برسناهان(Basso, Beattie and Bresnahan) مورد ارزیابی قرار گرفته اند. یافته ها: بر اساس ارزیابی بازیابی حرکتی موش¬ها، گروه تحت درمان با رزمارینیک اسید نسبت به گروه کنترل نمره بالاتری را کسب کردند. همچنین نتایج حاصل از بررسی مقاطع بافتی نشان دهنده بهبودی و ترمیم بهتر، کاهش حجم عارضه و افزایش میلینه شدن رشته های عصبی در محل آسیب نخاعی در گروه تحت درمان با رزمارینیک به صورت معنی داری نسبت به گروه کنترل شد (005/0 >p). بحث: نتایج حاصل از این پژوهش نشان دهنده اثرات ترمیمی رزمارینیک اسید در آسیب¬های نخاعی در موش¬های صحرایی می¬باشد که ممکن است بعنوان یک عامل درمانی برای درمان صدمات نخاعی مورد استفاده قرار گیرد.
Spinal cord injury is a condition in which the spinal cord's parenchyma is damaged due to trauma or different diseases. Inflammatory reactions can indeed play a significant role in causing spinal cord injuries by altering the function of cells and leading to the death of nerve cells. Rosmarinic acid, a water-soluble polyphenolic phytochemical, possesses antioxidant, anti-inflammatory and anti-apoptotic properties. In this study, 12 Wistar rats were divided in two groups with equal populations. The control group did not receive any treatment after spinal cord injury until day 56, while the rosmarinic acid group received 100 mg/kg of rosmarinic acid extract in their drinking water daily until day 56 after the spinal cord injury. During the 56-day period, the mice were assessed for movement using the Basso, Beattie, and Bresnahan test. The group treated with rosmarinic acid showed higher scores in terms of movement recovery compared to the control group.The examination of tissue sections revealed that the group treated with rosmarinic acid exhibited significantly better recovery and repair, a reduction in the size of complications, and an increase in the myelination of nerve fibers at the spinal cord injury site compared to the control group (p<0.005). The research findings suggest that rosmarinic acid could potentially be a valuable therapeutic option for treating spinal injuries in the future. It's exciting to see the potential restorative effects of this compound in experimental studies on rats. This could pave the way for further exploration and development of new treatment options for spinal injuries.
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