The Developmental Competence of the Bovine Embryos Processed in IVC-Stroebech Medium vs. Mcr2aa Medium
محورهای موضوعی : Large Ruminant Reproduction
M. Zandi
1
(Department of Agriculture, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran)
A. Ghaedrahmati
2
(Department of Animal Science, Agricultural Science and Natural Resources University of Khuzestan, Mollasani, Khuzestan, Iran)
L. Stroebech
3
(Department of Veterinary and Animal Science, University of Copenhagen, Denmark)
کلید واژه: blastocysts, define media, in vitro culture, in vitro fertilization, undefined media,
چکیده مقاله :
Fetal bovine serum (FBS) is preferred for its ability to provide a variety of molecules that can support the growth of embryos. However, the use of serum can lead to the development of large offspring syndrome, which is a significant problem. This study aimed to examine bovine embryo development derived from in vitro fertilized oocytes in two culture media: IVC-Stroebech medium, a chemically defined medium; and the modified medium of Charles Rosenkrans with amino acids (mCR2aa), an undefined medium containing serum. The process of in vitro maturation was carried out at 38.5 ˚C in IVC-Stroebech medium. The process of in vitro fertilization was carried out for 18 hours using Bracket and Oliphant's (BO) medium. As to the experiment, cumulus-free presumptive zygotes were randomly assigned to two different culture medium treatments. The first treatment was a two-step culture system using mCR2aa medium. The first step in-volved using mCR2aa medium without FBS for the first 48 hours of culture. After that, for the remaining days of culture, the second-step mCR2aa, which is composed of 10% FBS, replaced the medium. The em-bryos were incubated at 38.5 ˚C under 5% CO2 5% O2 and 90% N2 for 10 days. Statistical analysis re-vealed a significantly higher hatched blastocyst rate in the IVC-Stroebech medium in comparison with the mCR2aa medium. There was not any significant difference (P>0.05) in the formation rate of morulas and blastocysts between the IVC-Stroebech medium and the mCR2aa medium. Findings recommended the use of IVC-Stroebech medium for bovine in vitro embryo culture.
Fetal bovine serum (FBS) is preferred for its ability to provide a variety of molecules that can support the growth of embryos. However, the use of serum can lead to the development of large offspring syndrome, which is a significant problem. This study aimed to examine bovine embryo development derived from in vitro fertilized oocytes in two culture media: IVC-Stroebech medium, a chemically defined medium; and the modified medium of Charles Rosenkrans with amino acids (mCR2aa), an undefined medium containing serum. The process of in vitro maturation was carried out at 38.5 ˚C in IVC-Stroebech medium. The process of in vitro fertilization was carried out for 18 hours using Bracket and Oliphant's (BO) medium. As to the experiment, cumulus-free presumptive zygotes were randomly assigned to two different culture medium treatments. The first treatment was a two-step culture system using mCR2aa medium. The first step in-volved using mCR2aa medium without FBS for the first 48 hours of culture. After that, for the remaining days of culture, the second-step mCR2aa, which is composed of 10% FBS, replaced the medium. The em-bryos were incubated at 38.5 ˚C under 5% CO2 5% O2 and 90% N2 for 10 days. Statistical analysis re-vealed a significantly higher hatched blastocyst rate in the IVC-Stroebech medium in comparison with the mCR2aa medium. There was not any significant difference (P>0.05) in the formation rate of morulas and blastocysts between the IVC-Stroebech medium and the mCR2aa medium. Findings recommended the use of IVC-Stroebech medium for bovine in vitro embryo culture.
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