Isolation and Characterization of Antimicrobial Activities of Native Actinomycete Strains from Agricultural Soils in Guilan province
محورهای موضوعی : Biotechnological Journal of Environmental Microorganisms
الهام امیری
1
,
میر ساسان میر پور
2
,
خسرو عیسی زاده
3
,
بهنام راستی
4
1 - گروه میکروبیولوژی، دانشکده علوم پایه، دانشگاه آزاد اسلامی، واحد لاهیجان، لاهیجان، ایران
2 - گروه میکروبیولوژی، دانشکده علوم پایه، دانشگاه آزاد اسلامی، واحد لاهیجان، لاهیجان، ایران
3 - گروه میکروبیولوژی ، دانشکده علوم پایه، واحد لاهیجان، دانشگاه آزاد اسلامی، لاهیجان ، ایران
4 - گروه میکروبیولوژی، دانشکده علوم پایه، دانشگاه آزاد اسلامی، واحد لاهیجان، لاهیجان، ایران
کلید واژه: Actinomycete, Antimicrobial activity, 16S rRNA, Amycolatopsis roodepoortensis, Streptomyces,
چکیده مقاله :
Actinomycetes are known as the largest Source of natural antibiotics in the world. For this reason, due to their
ability to produce various antibiotics and other compounds of therapeutic importance, they are considered the
golden microorganisms of the 21st century. The purpose of this research is the isolation and molecular identification
of Actinomycete with antimicrobial properties from agricultural soils in the native areas of Guilan province.
Soil samples were collected from the southwestern agricultural areas of Guilan province. Serial dilution
was used to isolate Actinomycetes. Then the morphological, physiological, and biochemical identification of
the samples was done, and finally, the molecular identification of the isolates was conducted using 16S rRNA
sequencing and phylogenetic analysis. Antimicrobial activity was investigated against pathogenic microorganisms.
A total of 14 isolates were identified, and two isolates with stronger antimicrobial properties were selected.
Based on the results of phylogenetic studies and 16S rRNA sequencing, Amycolatopsis roodepoortensis
strain EA7 was identified with 99.63% confidence, and Streptomyces microflaveus strain EA6 with 93.92%
confidence. The isolated bacteria exhibited higher antimicrobial activity against Gram-positive pathogenic
microorganisms such as Staphylococcus aureus and the standard sample Staphylococcus aureus PTCC 1112.
This research represents the first report on the identification of Actinomycetes with antimicrobial properties
in the agricultural soils of the southwestern regions of Guilan province, located in the Alborz mountains. The
identification of the rare strain of Amycolatopsis roodepoortensis strain EA7 from the northern regions of Iran
highlights the significant value of the soils in these regions.
Actinomycetes are known as the largest Source of natural antibiotics in the world. For this reason, due to their
ability to produce various antibiotics and other compounds of therapeutic importance, they are considered the
golden microorganisms of the 21st century. The purpose of this research is the isolation and molecular identification
of Actinomycete with antimicrobial properties from agricultural soils in the native areas of Guilan province.
Soil samples were collected from the southwestern agricultural areas of Guilan province. Serial dilution
was used to isolate Actinomycetes. Then the morphological, physiological, and biochemical identification of
the samples was done, and finally, the molecular identification of the isolates was conducted using 16S rRNA
sequencing and phylogenetic analysis. Antimicrobial activity was investigated against pathogenic microorganisms.
A total of 14 isolates were identified, and two isolates with stronger antimicrobial properties were selected.
Based on the results of phylogenetic studies and 16S rRNA sequencing, Amycolatopsis roodepoortensis
strain EA7 was identified with 99.63% confidence, and Streptomyces microflaveus strain EA6 with 93.92%
confidence. The isolated bacteria exhibited higher antimicrobial activity against Gram-positive pathogenic
microorganisms such as Staphylococcus aureus and the standard sample Staphylococcus aureus PTCC 1112.
This research represents the first report on the identification of Actinomycetes with antimicrobial properties
in the agricultural soils of the southwestern regions of Guilan province, located in the Alborz mountains. The
identification of the rare strain of Amycolatopsis roodepoortensis strain EA7 from the northern regions of Iran
highlights the significant value of the soils in these regions.
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