بررسی متیلاسیون ژن 5 و10متیلن تتراهیدروفولات ردوکتاز در مردان نابارور استان کرمانشاه
محورهای موضوعی : فصلنامه زیست شناسی جانوری
طیبه امجدیان
1
,
پریچهره یغمایی
2
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نسيم حياتي روىباري
3
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خیراله یاری
4
1 - گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
2 - گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
3 - گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
4 - مرکز تحقیقات بیولوژی پزشکی، پژوهشکده فناوری های سلامت، دانشگاه علوم پزشکی کرمانشاه، کرمانشاه، ایران
کلید واژه: ناباروری مردان , متیلاسیون DNA, ژن MTHFR, qMSP ,
چکیده مقاله :
ناباروری یکی از شایع ترین مشکلات بهداشتی جهان است این مشکل حدود 15 درصد از زوج¬ها را درگیر می‌کند. متیلاسیون غیرطبیعی DNA اسپرم می تواند در میزان ناباروری مردان موثر باشد. 5 و10متیلن تتراهیدروفولات ردوکتاز (MTHFR) یک آنزیم حیاتی برای تنظیم الگوهای متیلاسیون DNA اسپرم و چرخه فولات است که نقش اساسی در فرایندهای اسپرماتوژنز ایفا می‌کند. تغییر در سطح بیان این ژن می¬تواند منجر به ناباروری مردان شود. هدف این مطالعه بررسی میزان متیلاسیون ژن MTHFR در مناطق متفاوت متیله شده (DMRs) و همبستگی بین الگوهای متیلاسیون DNA اسپرم با پارامترهای کیفیت مني در افراد بارور و نابارور است. نمونه‌های منی از 30 مرد نابارور و 15 مرد بارور جمع‌آوری شد. پس از تیمار DNA ها توسط بی سولفیت سدیم، وضعیت متیلاسیون MDRsژن MTHFR با روش qMSP ارزیابی شد. ارتباط معناداری بین متیلاسیون DMRژنMTHFR در گروه نابارور در مقایسه با بارور وجود دارد (036/0= p) و افزایش در مردان گروه نابارور مشاهده شد. علاوه بر این، روند کاهشی قابل توجهی در پارامترهای اسپرمی (غلظت اسپرم، تعداد، و مورفولوژی) در مردان گروه نابارور مشاهده شد. همچنین بین متیلاسیون DMRs ژن MTHFR و پارامترهای مایع منی در بیماران همبستگی منفی وجود داشت. هایپرمتیلاسیون ژن MTHFR در مناطق DMRs افراد نابارور با کاهش پارامترهای اسپرم مرتبط است.
Infertility is one of the most common health problems in the world, affecting about 15% of couples. Abnormal DNA methylation in sperm can be affective in male infertility. 5 and 10-methylene tetrahydrofolate reductase (MTHFR) is a vital enzyme for regulating DNA methylation patterns in sperm and the folate cycle, playing a crucial role in spermatogenesis processes. A Change in the expression level of this gene can lead to male infertility. This study aimed to investigate the level of methylation of the MTHFR gene in different methylated regions (DMRs) and the correlation between sperm DNA methylation patterns with semen quality parameters in fertile and infertile individuals. Semen samples were collected from 30 infertile men and 15 fertile men. After DNA treatment with sodium bisulfite, the methylation status of the MTHFR gene in DMRs was evaluated by qMSP method. There is a significant correlation between DMR methylation of the MTHFR gene in the infertile group compared to the fertile group (p = 0.036) and an increase was observed in infertile men. Additionally, a significant decrease in sperm parameters (sperm concentration, count, and morphology) was observed in the men of the infertile group. Also, there was a negative correlation between the methylation of DMRs of the MTHFR gene and semen parameters in patients. Hypermethylation of the MTHFR gene in DMRs in infertile individuals is associated with a decrease in sperm parameters.
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