بررسی اثر پيش آماده¬سازي قلبي با تمرين تناوبي شديد بر سطوح بافتي Hsp70 و آنزیم SOD به دنبال آنفارکتوس حاد میوکارد در موش های صحرایی نر
محورهای موضوعی : فصلنامه زیست شناسی جانوری
عبداله باقری
1
,
احمد همت فر
2
,
مهدی روزبهانی
3
,
ناصر بهپور
4
1 - گروه تربیت بدنی، واحد بروجرد، دانشگاه آزاد اسلامی، بروجرد، ایران
2 - گروه تربیت بدنی، واحد بروجرد، دانشگاه آزاد اسلامی، بروجرد، ایران
3 - گروه تربیت بدنی، واحد بروجرد، دانشگاه آزاد اسلامی، بروجرد، ایران
4 - گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه رازی، کرمانشاه، ایران
کلید واژه: پیش آماده‌سازی, تمرینات تناوبی شدید, Hsp70 , SOD, انفارکتوس حاد قلبی,
چکیده مقاله :
تمرینات ورزشی بهعنوان ابزاری برای افزایش توان محافظتی قلب در برابر استرسها محسوب میشود. هدف از انجام پژوهش حاضر بررسی اثر پيش آمادهسازي با تمرين تناوبي شديد بر Hsp70 و SOD میوکارد متعاقب انفاركتوس حاد قلبی در موشهاي صحرايي نر است. 20 سر موش 8 هفتهای نر با نژاد ویستار (با میانگین وزنی 1/5±41/224 گرم) به 4 گروه کنترل، تمرین، سکته و سکته-تمرین تقسیم شدند. گروههای تمرینی به دو هفته تمرین تناوبی شدید در چهار بخش پرداختند. بخش اول، سه روز تمرین هر روز دو جلسه و هر جلسه شامل 4 تناوب شدید دو دقیقهای با سرعت 35 تا 40 متر بر دقیقه که بین هر تناوب، یک وهله استراحت فعال 2 دقیقه ای با سرعت 25 تا 30 متر بر دقیقه در نظر گرفته شد. در بخش دوم، دو روز تمرین، هر روز دو جلسه تمرینی حاوی 4 تناوب فعالیت شدید 2 دقیقهای (40 تا 45 متر بر دقیقه) و 3 تناوب استراحت فعال 2 دقیقهای ( 28 تا 32 متر بر دقیقه) رسید. بخش سوم، نیز در سه روز تمرینی شامل 5 تناوب شدید (40 تا 45 متر بر دقیقه) و 4 تناوب استراحت فعال (28 تا 32 متر بر دقیقه) بود. بخش چهارم، شامل دو روز تمرینی با شدتی مشابه بخش سوم، امّا با افزایش یک تناوب فعالیت و استراحت فعال در هر جلسه همراه بود. در نهایت، Hsp70 و SOD به همراه میزان ناحیه انفارکته اندازهگیری شدند. نتایج آزمون تحلیل واریانس یک طرفه نشان داد که دو هفته تمرین تناوبی شدید موجب کاهش ناحیه انفارکته قلب موشها میشود و تغییرات معنیداری را در Hsp70 و SOD بهدنبال انفارکتوس حاد میوکارد بین گروههای سکته-تمرین و سکته ایجاد میکند. بنابراین، دو هفته تمرین HIIT از طریق مسیرهای سیگنالی، میتواند از قلب در برابر آسیب ناشی از ایسکمی حاد محافظت کند.
Sports training is considered as a tool to increase the protective power of the heart against stress.The aim of this study is to investigate the effect of pre-training with intense intermittent exercise on myocardial Hsp70 and SOD following acute myocardial infarction in male rats. 20 8-week-old male Wistar rats (with an average weight of 224.41±5.1 grams) were divided into 4 groups: control, exercise, stroke, and stroke-exercise. The training groups did two weeks of intense interval training in four sections. The first part, three days of training every day, two sessions, each day, and each session includes 4 intense intervals of two minutes at a speed of 35 to 40 meters per minute, and between each interval, a 2 minute active rest period at a speed of 25 to 30 meters per minute. Considered in the second part, two days of training, each day consisted of two training sessions containing 4 periods of intense activity of 2 minutes (40 to 45 m/min) and 3 periods of active rest of 2 minutes (28 to 32 m/min). The third part also included 5 intense periods (40 to 45 m/min) and 4 active rest periods (28 to 32 m/min) in three training days. The fourth part included two training days with the same intensity as the third part, but with an increase in activity and active rest in each session. Finally, Hsp70 and SOD were measured along with the amount of infarcted area. Heart damage enzymes (CK and LDH) were also measured in serum. The one-way analysis of variance test showed that two weeks of intense intermittent exercise, although it reduces the infarcted area of the heart of rats, but there were significant changes in Hsp70 and SOD after acute myocardial infarction between the stroke-exercise and stroke groups. Does not cause, while the measured heart damage enzymes (CK and LDH) showed a significant decrease in the stroke-exercise group compared to the stroke group. Therefore, two weeks of HIIT training can protect the heart from acute ischemia-induced damage through signaling pathways.
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