اثر هشت هفته تمرین تناوبی شدید بر بیان ژن هایICAM و CRPدر بطن چپ رت‌های چاق
محورهای موضوعی : فصلنامه زیست شناسی جانوری
رسول فتوحی اردهایی
1
(گروه فیزیولوژی ورزش، واحد علی آباد کتول، دانشگاه آزاد اسلامی، علی آباد کتول، ایران)
ندا آقایی بهمن بگلو
2
(گروه فیزیولوژی ورزش، واحد علی آباد کتول، دانشگاه آزاد اسلامی، علی آباد کتول، ایران)
رضا رضایی شیرازی
3
(گروه فیزیولوژی ورزش، واحد علی آباد کتول، دانشگاه آزاد اسلامی، علی آباد کتول، ایران)
مجتبی ایزدی
4
(گروه فیزیولوژی ورزش، واحد علی آباد کتول، دانشگاه آزاد اسلامی، علی آباد کتول، ایران)
کلید واژه: چاقی, تمرین تناوبی شدید, بیان ژن, ICAM , CRP, رت‌های چاق,
چکیده مقاله :
نقش چاقی در بیماری‌های مزمن نظیر دیابت نوع 2 و بیماری‌های قلبی-عروقی به خوبی مشخص شده است. این مطالعه با هدف تعیین اثر هشت هفته تمرین تناوبی شدید بر بیان ژن های CRP و ICAM در بطن چپ رت‌های چاق انجام گرفت. جامعه آماری مطالعه حاضر را رت‌های نر ویستار انستیتو پاستور تهران تشکیل می‌دهند. 21 سر رت نر ویستار انتخاب شدند و پس از القای چاقی به سه گروه نرمال، چاق کنترل و چاق تناوبی تقسیم شدند. برای القای چاقی از شش هفته رژیم غذایی پرچرب استفاده شد. رت‌های گروه ورزش یک برنامه تمرینات تناوبی 8 هفته‌ای را در قالب دویدن‌ها تناوبی روی تریدمیل اجرا نمودند. گروه نرمال و چاق کنترل در برنامه تمرین شرکت نداشتند. 48 ساعت پس از آخرین جلسه تمرین، تشریح انجام شد. از آزمون آماری تی مستقل جهت مقایسه پیش آزمون‌ها و از آزمون تی همبسته نیز جهت تعیین سطح تغییرات درون گروهی در سطح معناداری استفاده شد. تمرین تناوبی بیان ICAM را نسبت به گروه چاق کنترل به میزان معنی داری کاهش داد (0001/0 > p) بطوریکه سطوح آن به گروه نرمال نزدیک شد. به عبارتی تفاوت معنی¬داری در بیان ICAM بین گروه چاق تناوبی و گروه نرمال مشاهده نشد. بیان CRP بواسطه تمرین تناوبی نسبت به گروه چاق کنترل به میزان معنی¬داری کاهش یافت بطوریکه سطوح آن به گروه نرمال نزدیک گردید. تفاوت معنی داری در بیان CRP بین گروه چاق تناوبی و گروه نرمال مشاهده نگردید. تمرین تناوبی شدید موجب کاهش ICAM و CRP نسبت به گروه کنترل شد. احتمالاً، ً تمرین تناوبی با افزایش فعالیت سیستم سمپاتیک و افزایش سایتوکاین¬های ضد التهابی، رهایش میانجی‌های التهابی از بافت چربی را مهار کرده و به دنبال آن، غلظت مولکول‌های چسبان سلولی کاهش می‌یابد.
The role of obesity in chronic diseases such as type 2 diabetes and cardiovascular diseases is well known. This study was conducted to determine the effect of eight weeks of intense intermittent exercise on the expression of CRP and ICAM genes in the left ventricle of obese rats. The statistical population of the current study consists of male Wistar rats from the Pasteur Institute in Tehran. 21 male Wistar rats were selected and after induction of obesity, they were divided into three groups: normal, obese control, and periodic obese. Six weeks of a high-fat diet was used to induce obesity. The rats in the exercise group performed an 8-week interval training program in the form of interval runs on the treadmill. The normal and obese control group did not participate in the exercise program. 48 hours after the last training session, dissection was performed. The independent t-test was used to compare the pre-tests and the correlated t-test was used to determine the level of intra-group changes at a significant level. Intermittent training significantly reduced the expression of ICAM compared to the obese control group (p < 0.0001) so that its levels were close to the normal group. In other words, there was no significant difference in the expression of ICAM between the intermittently obese group and the normal group. The expression of CRP decreased significantly due to intermittent exercise compared to the obese control group, so its levels were close to the normal group. There was no significant difference in the expression of CRP between the intermittently obese group and the normal group. Intense intermittent exercise decreased ICAM and CRP compared to the control group. Probably, intermittent training inhibits the release of inflammatory mediators from fat tissue by increasing the activity of the sympathetic system and increasing anti-inflammatory cytokines, and subsequently, the concentration of cell adhesion molecules decreases.
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