بررسی اثر ضد اضطرابی عصاره هیدروالکلی خرفه (Oleracea Portulaca L.) در موش صحرایی در طی سمیت با بیسفنول A

محورهای موضوعی : فصلنامه زیست شناسی جانوری

داود فضلی ¹ , غلامحسن واعظی ² , بهناز برزگرزاده نمرور ³ , یوسف علیپور علیایی ⁴

1 - گروه زیست شناسی، دانشکده علوم پایه، دانشگاه پیام‌نور، تهران، ایران
2 - گروه زیست شناسی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران
3 - گروه زیست شناسی جانوری، دانشکده علوم طبیعی، دانشگاه تبریز، تبریز، ایران
4 - گروه زیست شناسی جانوری، دانشکده علوم طبیعی، دانشگاه تبریز، تبریز، ایران

تاریخ دریافت : 1400/08/28 تاریخ پذیرش : 1400/10/20 تاریخ انتشار : 1401/09/01

کلید واژه: اضطراب, خرفه, بیسفنول, ماز بعلاوه‌ای شکل مرتفع,

چکیده مقاله :

ب بیسفنول A یک ماده شیمیایی بالقوه مختل کننده غدد درون ریز است که در صنایع پلاستیک بصورت وسیع مورد استفاده قرار می گیرد و اثرات مخرب آن بر سیستم های مختلف بدن اثبات شده است.. انسان بدلیل اینکه این ماده می تواند از مواد پلیمری آزاد شود و وارد منابع آبی و غذایی گردد، در معرض خطر بیشتری قرار دارد. گیاه خرفه (Oleracea Portulaca L.) با دارا بودن انواع ترکیبات فیتوشیمیایی با خاصیت آنتی اکسیدانی و ضد التهابی می تواند اثرات سمی بیسفنول را تعدیل نموده و بهبود بخشد. در این تحقیق، 49 سر رت نر بالغ نژاد ویستار به صورت تصادفی در هفت گروه 7 تایی به شرح زیر تقسیم شدند: گروه کنترل، شاهد (دریافت کننده500 میکروگرم روغن ذرت/کیلوگرم وزن بدن)، گروه دریافت کننده سم (500 میکروگرم/کیلوگرم وزن بدن بیسفنول A)، کنترل مثبت (100 میلی گرم/کیلوگرم وزن بدن عصاره هیدروالکلی گیاه خرفه)، 3 گروه تیمار دریافت کننده خرفه (500 میکروگرم بیسفنولA + 50، 100، 150 میلی گرم عصاره هیدروالکلی گیاه خرفه/کیلوگرم وزن بدن). تزریق ها به صورت داخل صفاقی و به مدت 14 روز پیاپی انجام گردید. تست اضطراب توسط ماز بعلاوه ای شکل مرتفع در روز آخر انجام گرفت. داده ها در نرم افزار آماری SPSS طی آنالیز واریانس یک طرفه با تست تعقیبی توکی مورد تجزیه و تحلیل قرار گرفت. این تحقیق نشان داد در گروه‌های تیمار شده با خرفه زمان سپری شده در بازوی باز (OAT) و درصد دفعات ورود به بازوی باز (OAE) به‌عنوان شاخص های اضطراب افزایش معنی‌داری (05/0p < ) را در مقایسه با گروه کنترل دارد. نتایج حاکی از اثر وابسته به دوز عصاره هیدروالکلی خرفه در کاهش حالت اضطراب است. همچنین داده های تجزیه و تحلیل نشان داد که خرفه می تواند اثرات سمی BPA را بهبود بخشد.

چکیده انگلیسی:

Bisphenol A is a potentially endocrine-disrupting chemical agent which is widely used in plastic industries and its wrecking effects on various body organs have been proved. Human is more exposed to BPA because it can be released from polymeric materials and enter water and food sources. Purslane is a plant with different kinds of phytochemical compounds and antioxidant and anti-inflammatory effects could modify and improve the toxic effects of BPA. Forty nine rats were randomly divided into 7 groups: control group, experiment group (receiving 500 µg of corn oil / kg BW), toxin-receiving group (500 µg of bisphenol A/kg BW), positive control (100 mg of hydroalcoholic extract of portulaca oleracea/kg BW), 3 treatment groups receiving purslane hydroalcoholic extract (500 μg of bisphenol A + 50, 100, 150 mg of hydroalcoholic extract of purslane hydroalcoholic extract/ kg BW, respectively). All injections are done by i.p rout in 14 consecutive days. Anxiety test is performed by elevated plus maze apparatus in the last day. Data was analyzed in SPSS statistical software by one-way ANOVA with Tukey posttest. The findings of the study indicated a significant increase (p ≤ 0.05) in the amount of Open Arm Time (OAT) and Open Arm Entries (OAE) as anxiety parameters in purslane treated groups compared with control group. The results indicate the dose-dependent effect of hydroalcoholic extract of purslane on the reduction of anxiety state. Also analyzed data showed that purslane can improve BPA toxic effects.

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