تعامل اسید گالیک و تمرین شنا بر هیستولوژی تخمدان در موش‌های صحرایی مبتلا به سندرم تخمدان پلی‌کیستیک

محورهای موضوعی : فصلنامه زیست شناسی جانوری

مریم ابراهیمی ¹ , نرگس ابرشهر ² , محمد امین عدالت منش ³

1 - گروه زیست شناسی، دانشکده علوم، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران
2 - گروه زیست شناسی، دانشکده علوم، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران
3 - گروه زیست شناسی، دانشکده علوم، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران

تاریخ دریافت : 1400/09/27 تاریخ پذیرش : 1400/11/02 تاریخ انتشار : 1401/09/01

کلید واژه: موش صحرایی, اسید گالیک, تمرین شنا, سندرم تخمدان پلی‌کیستیک,

چکیده مقاله :

سندرم تخمدان پلی‌کیستیک (PCOS) علت اصلی ناباروری ناشی از فقدان تخمک‌گذاری و نتیجه استرس اکسایشی بافت تخمدان است که با اختلال در عملکرد تخمدان همراه می‌باشد. هدف از پژوهش حاضر ارزیابی اثر 4 هفته تجویز گالیک اسید (GA) و تمرین شنا (EX) بر پارامترهای هیستولوژیک تخمدان در مدل PCOS می‌باشد. تعداد 40 سر موش صحرایی ماده بالغ نژاد ویستار با وزن 10 ± 180 گرم به‌طور تصادفی در 5 گروه کنترل،PCOS ، GA + PCOS، PCOS + EX و PCOS + GA + EX قرار گرفتند. برای ایجاد مدل PCOS، لتروزول (1 میلی گرم بر کییلوگرم) به مدت 28 روز گاواژ شد. سپس، تجویز خوراکی اسید گالیک به مدت 4 هفته و تمرینات شنا 3 جلسه در هفته و به مدت 4 هفته انجام شد. در پایان، پس از تشریح تخمدان و رنگ‌آمیزی با هماتوکسی‌لین-ائوزین، ارزیابی‌‌های بافتی صورت گرفت. نتایج کاهش معنادار قطر جسم زرد، تعداد فولیکول‌های تک لایه، چند لایه، ثانویه، گراف و جسم زرد در گروه PCOS و نیز افزایش معنی‌دار تعداد فولیکول‌های آترتیک و کیستیک را نسبت به گروه کنترل نشان داد. در حالی که تعامل تجویز اسید گالیک و تمرین شنا سبب بهبود شاخص‌های تخمدان گردید. با این حال، تغییر معنا‌داری در قطر اووسیت و فولیکول‌های بدوی و چند لایه در گروه‌های مطالعه مشاهده نشد. به نظر می رسد گالیک اسید و تمرین شنا در بازگرداندن فولیکولوژنز موش‌های صحرایی مبتلا به PCOS و بر روند تخمک‌گذاری موثر واقع شوند.

چکیده انگلیسی:

Polycystic Ovary Syndrome (PCOS) is the leading cause of infertility due to lack of ovulation and the result of oxidative stress of ovarian tissue, which is associated with disability in ovarian functions. This study aims at evaluating the effect of 4 weeks of Gallic Acid (GA) prescription and swimming (EX) training on ovarian tissue parameters in PCOS model. Forty adult Wistar female rats weighing 180 ± 10 g were randomly divided into 5 groups: PCOS, PCOS + GA, PCOS + EX and PCOS + GA + EX. To induce the PCOS model, letrozole (1 mg / kg) was NG tubed for 28 days. Then, GA was prescribed orally for 4 weeks and swimming was performed for 4 weeks and 3 sessions per week. Finally, after dissection of the ovary and staining with hematoxylin-eosin, histological evaluations were performed. The results showed a significant decrease in corpus luteum diameter, number of monolayer, multilayer, secondary and Graafian follicles and corpus luteum in PCOS group and also a significant increase in the number of atretic and cystic follicles compared with control group. While the interaction of GA prescription and swimming improved ovarian parameters. However, no significant change in the diameter of oocytes and primary and multilayered follicles was observed in the groups. GA and swimming appear to be effective on restoring the folliculogenesis of PCOS rat model and the ovulation process.

منابع و مأخذ:

1. Abdel-Moneim A., El-Twab S.M.A., Yousef A.I., Reheim E.S.A., Ashour M.B. 2018. Modulation of hyperglycemia and dyslipidemia in experimental type 2 diabetes by gallic acid and p-coumaric acid: The role of adipocytokines and PPARγ. Biomedicine and Pharmacotherapy, 105:1091-1097.


2. Agarwal A., Aponte-Mellado A., Premkumar B.J., Shaman A., Gupta S. 2012. The effects of oxidative stress on female reproduction: a review. Reproductive Biology and Endocrinology, 2012;10:49.


3. Azziz R. 2018. Polycystic Ovary Syndrome. Obstetrics and Gynecology 132(2):321-336.


4. Barber T.M., Dimitriadis G.K., Andreou A., Franks S. 2015. Polycystic ovary syndrome: insight into pathogenesis and a common association with insulin resistance. Clinical Medicine (Lond), 16(3):262-266.


5. Barber T.M., Franks S. 2021. Obesity and polycystic ovary syndrome. Clinical Endocrinolology (Oxf), 95(4):531-541.


6. Barber T.M., Hanson P., Weickert M.O., Franks S. 2019. Obesity and Polycystic Ovary Syndrome: Implications for Pathogenesis and Novel Management Strategies. Clinical Medicine Insights: Reproductive Health, 13: 1179558119874042.


7. Choubey S., Goyal S., Varughese L.R., Kumar V., Sharma A.K., Beniwal V. 2018. Probing Gallic Acid for Its Broad Spectrum Applications. Mini-Reviws in Medicinal Chemistry, 18(15):1283-1293.


8. Choubey S,, Varughese L.R., Kumar V., Beniwal V. 2015. Medicinal importance of gallic acid and its ester derivatives: a patent review. Pharmaceutical Patent Analyst, 4(4):305-315.


9. Corrie L., Gulati M., Singh S.K., Kapoor B., Khursheed R., Awasthi A. 2021. Recent updates on animal models for understanding the etiopathogenesis of polycystic ovarian syndrome. Life Science, 280:119753.


10. Guo Y., Qi Y., Yang X., Zhao L., Wen S., Liu Y., Tang L. 2016. Association between polycystic ovary syndrome and gut microbiota. PLoS One, 11(4): e0153196.


11. Hakimi O., Cameron L.C. 2017. Effect of Exercise on Ovulation: A Systematic Review. Sports Medicine, 47(8):1555-1567.


12. Hyderali B.N., Mala K. 2015. Oxidative stress and cardiovascular complications in polycystic ovarian syndrome. European Journal of Obstetrics and Gynecology and Reproductive Biology, 191:15-22.


13. Jelodar G., Masoomi S., Rahmanifar F. 2018. Hydroalcoholic extract of flaxseed improves polycystic ovary syndrome in a rat model. Iranian Journal of Basic Medical Sciences, 21(6):645–650.


14. Li W., Li D., Kuang H., Feng X., Ai W., Wang Y., 2020. Berberine increases glucose uptake and intracellular ROS levels by promoting Sirtuin 3 ubiquitination. Biomedicine and Pharmacotherapy, 121: 109563.


15. Maliqueo M., Benrick A., Stener-Victorin E. 2014. Rodent models of polycystic ovary syndrome: phenotypic presentation, pathophysiology, and the effects of different interventions. Seminars in Reproductive Medicine, 32(3):183-193.


16. Manni L., Cajander S., Lundeberg T., Naylor A.S., Aloe L., Holmäng A., 2005. Effect of exercise on ovarian morphology and expression of nerve growth factor and alpha(1)- and beta(2)-adrenergic receptors in rats with steroid-induced polycystic ovaries. Journal of Neuroendocrinology, 17(12):846-858.


17. Matsuzaki T., Tungalagsuvd A., Iwasa T., Munkhzaya M., Yanagihara R., Tokui T., 2017. Kisspeptin mRNA expression is increased in the posterior hypothalamus in the rat model of polycystic ovary syndrome. Endocrine Journal, 64(1):7–14.


18. Mazloom B.F., Edalatmanesh M.A., Hosseini S.E. 2019. Gallic acid reduces inflammatory cytokines and markers of oxidative damage in a rat model of estradiol-induced polycystic ovary. Comparative Clinical Pathology, 28: 1281-1286.


19. Mehraban M., Jelodar G., Rahmanifar F. 2020. A combination of spearmint and flaxseed extract improved endocrine and histomorphology of ovary in experimental PCOS. Journal of Ovarian Research, 13(1): 32.


20. Moghadas M., Edalatmanesh M.A., Robati R. 2016. Histopathological analysis from gallic acid administration on hippocampal cell density, depression, and anxiety related behaviors in a trimethyltin intoxication model. Cell Journal, 17(4): 659-667.


21. Moghtaderi H., Sepehri H., Delphi L., Attari F. 2018. Gallic acid and curcumin induce cytotoxicity and apoptosis in human breast cancer cell MDA-MB-231. Bioimpacts, 8(3):185-194.


22. Mohammadi M. 2019. Oxidative stress and polycystic ovary syndrome: A brief review. International Journal of Preventive Medicine, 17(10):86.


23. Noroozzadeh M., Behboudi-Gandevani S., Zadeh-Vakili A., Ramezani Tehrani F. 2017. Hormone-induced rat model of polycystic ovary syndrome: A systematic review. Life Science, 191:259-272.


24. Qiu S., Wu C., Lin F., Chen L., Huang Z., Jiang Z. 2009. Exercise training improved insulin sensitivity and ovarian morphology in rats with polycystic ovary syndrome. Hormone and Metabolic Research, 41(12): 880-885.


25. Rafiei S., Edalatmanesh M.A. 2018. The effect of swimming training, Gallic acid and high-fat diet on the serum levels of sex hormones in rats with polycystic ovary syndrome. Feyz, 22(6):564-572. [Persian]


26. Rafiei S., Ravasi A.A., Gaeini A.A. 2021. The effect of eight-week swimming exercise and gallic acid on hippocampal BDNF and oxidative stress parameters in Trimethyltin induced cognitive deficits. Jorjani Biomedicine Journal, 9(1): 55-68.


27. Roidoung S., Dolan K.D., Siddiq M. 2016. Gallic acid as a protective antioxidant against anthocyanin degradation and color loss in vitamin-C fortified cranberry juice. Food Chemistry, 210:422-427.


28. Rosenfield R.L. 2015. The polycystic ovary morphology-polycystic ovary syndrome spectrum. Journal of Pediatrics and Adolescent Gynecology, 28(6):412-419.


29. Sanchez-Garrido M.A., Tena-Sempere M. 2020. Metabolic dysfunction in polycystic ovary syndrome: Pathogenic role of androgen excess and potential therapeutic strategies. Molecular Metabolism, 35:100937.


30. Stepto N.K., Hiam D., Gibson-Helm M., Cassar S., Harrison C.L., Hutchison S.K. 2020. Exercise and insulin resistance in PCOS: muscle insulin signalling and fibrosis. Endocrine Connection, 9(4):346-359.


31. Woodward A., Klonizakis M., Broom D. 2020. Exercise and polycystic ovary syndrome. Advances in Experimental Medicine and Biology, 1228:123-136.


32. Wu C., Lin F., Qiu S., Jiang Z. 2014. The characterization of obese polycystic ovary syndrome rat model suitable for exercise intervention. PLoS One, 9(6): e99155.


33. Zeng X., Xie Y.J., Liu Y.T., Long S.L., Mo Z.C. 2020. Polycystic ovarian syndrome: correlation between hyperandrogenism, insulin resistance and obesity. Clinica Chimica Acta, 502:214-221.

34. Zuo T., Zhu M., Xu W. 2016. Roles of oxidative stress in polycystic ovary syndrome and cancers. Oxidative Medicine and Cellular Longevity, 2016:8589318.