بررسی زیست سازگاری داربست PLA پوشش داده شده با لاپونیت بر روی سلول های بنیادی مزانشیمی مغز استخوان انسان

محورهای موضوعی : فصلنامه زیست شناسی جانوری

زهرا عرفا ¹ , شیوا ایرانی ² , علی زمانیان ³ , هادی بخشی ⁴ , حبیب نیکوکار ⁵ , به آفرید قلندری ⁶

1 - گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
2 - گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
3 - گروه بیومواد، پژوهشکده فناوری نانو و مواد پیشرفته، پژوهشگاه مواد و انرژی، کرج، ایران
4 - پژوهشکده سیستم‌های پلیمری عامل، موسسه فرانهوفر برای تحقیقات پلیمری کاربردی، پوتسدام، آلمان
5 - مرکز تحقیقات نانو تکنولوژی پزشکی و مهندسی بافت، پژوهشکده علوم تولید مثل یزد، دانشگاه علوم پزشکی و خدمات بهداشتی درمانی شهید صدوقی یزد، یزد، ایران
6 - گروه نانوتکنولوژی پزشکی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

تاریخ دریافت : 1399/11/18 تاریخ پذیرش : 1399/01/21 تاریخ انتشار : 1400/06/01

کلید واژه: مهندسی بافت, الکتروریسی, استخوان, پلی‌لاکتیک اسید, لاپونیت,

چکیده مقاله :

مهندسی بافت استخوان رویکردی امیدوارانه جهت توسعه درمان های مناسب جدید برای رفع آسیب های بافت استخوانی است. یکی از اهداف مهم در این رشته، ساخت داربست هایی با تقلید از ماتریکس خارج سلولی است. هدف از این مطالعه تولید داربست پلی لاکتیک اسید/لاپونیت (PLA/LAP) و بررسی رفتار سلول های بنیادی مزانشیمی مغز استخوان انسانی (hBMSCs) بر روی آن بود. ابتدا داربست PLA به روش الکتروریسی ساخته شد و سپس LAP با غلظت 8/0 درصد وزنی (LAP0.8%) بر روی آن پوشش داده شد. مورفولوژی داربست توسط میکروسکوپ الکترونی روبشی (SEM) و طیف سنجی پراش انرژی پرتو ایکس (EDX)، ساختار شیمیایی آن توسط طیف سنجی ATR-FTIR و میزان آبدوستی داربست با آزمون زاویه تماس آب بررسی شد. نهایتاً زیست سازگاری داربست و بقای سلولی توسط تست MTT، بر روی سلول های hBMSC انجام شد. نتایج حاصل از مورفولوژی داربست نشان دهنده پوشش دهی موفق LAP0.8% بر روی داربست PLA بود. همچنین، سطح آبدوستی داربست PLA پس از پوشش دهی با LAP بهبود یافت. زیست سازگاری داربست تا 24 ساعت بعد از کشت سلولی و بقای سلول های hBMSC تا 72 ساعت پس از کشت (001/0 ≥ p ) تایید شد. از نتایج بدست آمده در این تحقیق به نظر می رسد که داربست PLA/LAP0.8%بدلیل حفظ زیست سازگاری و بقای سلولی که بواسطه حضور یون های موجود در نانوذره LAP است، می تواند کاندیدای مناسبی برای برنامه های مهندسی بافت استخوان باشد.

چکیده انگلیسی:

Bone tissue engineering is a promising approach to develop new appropriate treatments for bone tissue damage. One of the important goals in this field is to fabricate the scaffolds by mimicking the extracellular matrix. The aim of this study was to study the fabrication of polylactic acid/Laponite (PLA/LAP) scaffold and to investigate the behavior of human bone marrow mesenchymal stem cells (hBMSCs) on it. First, PLA scaffold was fabricated by electrospinning technique, and then LAP (0.8 wt%) was coated on it. The morphology of the scaffold was examined by scanning electron microscopy (SEM) and Energy-dispersive X-ray (EDX) spectroscopy. The chemical structure of the scaffold was evaluated by ATR-FTIR spectroscopy and its hydrophilicity was tested by measuring the water contact angle. Finally, the biocompatibility of the scaffold and cell viability tested with MTT assay was performed on hBMSCs. The results of scaffold morphology showed a successful coat of LAP 0.8% on the surface of PLA scaffold. Furthermore, the hydrophilicity of PLA scaffold improved after coating with LAP 0.8%. The Biocompatibility of scaffold up to 24 hours and hMSCs viability up to 72 hours after cell culture were confirmed (p≤0.001). Based on the results of this study, it seems that PLA/LAP of 0.8% scaffold can be a promising candidate for bone tissue engineering applications by maintaining biocompatibility and cell viability due to the presence of ions in LAP nanoparticles.

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