بررسی نقش گیرنده های GABAA و GABAB در رفتارهای شبه اضطرابی ناشی از لیپوپلی ساکاریدها در آزمون جعبه تاریک و روشن در موش های سوری نر
محورهای موضوعی : فصلنامه زیست شناسی جانوری
معصومه علیشاهی
1
,
مریم بنانج
2
,
جلال صولتی
3
,
رامین حاجی خانی
4
,
مصطفی قادری
5
1 - گروه زیست شناسی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران
2 - گروه زیست شناسی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران
3 - گروه زیست شناسی، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران
4 - گروه زیست شناسی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران
5 - گروه میکروبیولوژی، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران
کلید واژه: لیپوپلی ساکارید, رفتار شبه اضطرابی, گابا A, گابا B, جعبه تاریک و روشن,
چکیده مقاله :
سیستم گاباارژیک یک سیستم ضد اضطراب در مغز محسوب می شود. بنابراین هدف از این مطالعه بررسی نقش گیرنده های GABAA و GABAB در رفتارهای شبه اضطرابی ناشی از لیپوپلی ساکاریدها در موش های سوری نر می باشد. در این آزمایش موش ها 2 ساعت بعد از تزریق LPS موسیمول (05/0، 1/0 و 2/0 میکروگرم بر موش)، بیکوکولین (25/0، 5/0 و 1 میکروگرم بر موش)، باکلوفن (1، 2 و 4 میکروگرم بر موش)، CGP (8/0, 4/0 , 2/ 0میکروگرم بر موش) و Celebrex را بصورت درون بطنی دریافت کردند. 5 دقیقه بعد از تزریق درون بطنی تست LDB انجام گرفت. این مطالعه نشان داد که موسیمول موجب کاهش معنی دار رفتار اضطرابی در آزمون LDB شد (05/0 > p ). اما تجویز موسیمول و LPS تغییر معنی داری نداشته است (P≥0.05). دوزهای مختلف بیکوکولین و LPS موجب افزایش معنی دار رفتار اضطرابی در آزمون LDB شد (05/0 > p ). باکلوفن وCGP به تنهایی و همراه با LPS تغییر معنی داری در رفتار اضطرابی حیوان در آزمون LDB نداشته است (05/0 > p ). تزریق Celebrex متعاقب LPS موجب رفع رفتار شبه اضطرابی ناشی از تزریق LPS شد. به طور کلی میتوان گفت رفتارهای شبه اضطرابی در حیوانات دریافت کننده LPS ممکن است ناشی از مهار گیرنده های GABAAو GABABو افزایش سطح فاکتورهای التهابی بافت مغز بوده باشد.
Thegabaergic system is considered as the anti-anxiety system in the brain. The aim of this study was to investigate the role of GABAA and GABAB receptors in anxiety-like behaviors caused by lipopolysaccharides in male mice. In this test,mice were injected with lipopolysaccharide (LPS) (0.2 mg/kg), then injected with either muscimol (0.05, 0.1 or 0.2 µg/mouse), bicuculline (0.25, 0.5 or1 µg/mouse), baclofen (1, 2 or 4 µg/mouse) or CGP (0.2, 0.4 or 0.8 µg/mouse) 2 hours later and received intraventricular Celebrex. Five minutes after intraventricular injection, a dark and light box test was performed. The results of this study showed that musimol significantly reduced anxiety-like behavior in the LDB test (p < 0.05). The results of this study showed that musimol significantly reduced anxiety-like behavior in the LDB test (p < 0.05). However, the administration of musimol and LPS did not significantly change the animal’s anxiety-like behavior (p ≥ 0.05). Different doses of bioculin and LPS significantly increased the anxiety-like behavior in the LDB test (p < 0.05). Baclofen and CGP alone and with LPS did not significantly change the animal’s anxiety-like behavior on the LDB test (p ≥ 0.05). Celebrex injection after LPS relieved the anxiety-like behavior caused by LPS injection. In general, it can be claimed that anxiety-like behaviors in animals receiving LPS may be due to inhibition of GABAA and GABAB receptors and increase in the level of inflammatory factors in brain tissue.
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