Preparation of Efficient Kit for the Semi-Quantitative Determination of Sarcosine as a cancer marker by Grafting Molecularly Imprinted-Stationary on Glass Plate

محورهای موضوعی :

Nahid Farhadgholami ¹ , Hamid Hashemi-Moghaddam ² , Masoud Shaabanzadeh ³ , Saeed Zavareh ⁴

1 - Department of Chemical Engeenering, Damghan Branch, Islamic Azad University, Damghan, Iran
2 - Department of Chemistry, Damghan Branch, Islamic Azad University, Damghan, Iran
3 - Department of Chemistry, Damghan Branch, Islamic Azad University, Damghan, Iran
4 - Department of Biology, Damghan University, Damghan, Iran

تاریخ دریافت : 1399/02/16 تاریخ پذیرش : 1399/08/30 تاریخ انتشار : 1402/03/11

کلید واژه: grafting, Sarcosine, Imprinted polymer, Kit,

چکیده مقاله :

This paper presents a novel, rapid, and simple method for the determination of sarcosine. The surface of a glass plate was modified with 3-(methacryloxy)propyltrimethoxysilane. Then, a sarcosine-imprinted polymer was grafted on the glass plate by copolymerization of the vinyl end groups with a functional monomer and a cross-linking agent. The synthesized polymers were characterized by Fourier transform infrared spectroscopy and scanning electron microscopy. In the subsequent step, the determination of sarcosine was conducted using the synthesized kit in optimized conditions. The synthesized grafted plate was able to absorb sarcosine selectively in the presence of other amino acids, showing that the proposed method enabled the rapid determination of sarcosine.

منابع و مأخذ:

1. Sboner A., Demichelis F., Calza S., Pawitan Y., Setlur S.R., Hoshida Y., Perner S., Adami H.O., Fall K., Mucci L.A., 2010. Molecular sampling of prostate cancer: a dilemma for predicting disease progression. BMC Medical Genomics. 3(1), 1-12.


2. Cuzick J., Thorat M. A., Andriole G., Brawley O. W., Brown P. H., Culig Z., Eeles R.A., Ford L.G., Hamdy F.C., Holmberg L., 2014. Prevention and early detection of prostate cancer. The Lancet Oncology. 15(11), e484-e492.


3. Salciccia S., Capriotti A.L., Laganà A., Fais S., Logozzi M., De Berardinis E., Busetto G.M., Di Pierro G.B., Ricciuti G.P., Del Giudice F., 2021. Biomarkers in Prostate Cancer Diagnosis: From Current Knowledge to the Role of Metabolomics and Exosomes. International Journal of Molecular Sciences. 22(9), 4367.


4. Friedman M., 2004. Applications of the ninhydrin reaction for analysis of amino acids, peptides, and proteins to agricultural and biomedical sciences. Journal of Agricultural and Food Chemistry. 52(3), 385-406.


5. Meyer T.E., Fox S.D., Issaq H.J., Xu X., Chu L.W., Veenstra T.D., Hsing A.W., 2011. A reproducible and high-throughput HPLC/MS method to separate sarcosine from α-and β-alanine and to quantify sarcosine in human serum and urine. Analytical Chemistry. 83(14), 5735-5740.


6. Chen S.E., Hu J., Yan P., Sun J., Jia W., Zhu S., Zhao X.E., Liu H., 2021. 12-Plex UHPLC-MS/MS analysis of sarcosine in human urine using integrated principle of multiplex tags chemical isotope labeling and selective imprint enriching. Talanta. 224, 121788.


7. Yamkamon V., Yee P.P., Yainoi S., Eiamphungporn W., Suksrichavalit T., 2018. Simultaneous determination of sarcosine and its related metabolites by gas chromatography-tandem mass spectrometry for prostate cancer diagnosis. EXCLI Journal. 17, 965.


8. Hashemi-Moghaddam H., Hagigatgoo M., 2015. Nonderivatized Sarcosine Analysis by Gas Chromatography after Solid-Phase Microextraction by Newly Synthesized Monolithic Molecularly Imprinted Polymer. Chromatographia. 78(19-20), 1263-1270.


9. Hashemi-Moghaddam H., Rahimian M., Niromand B., 2013. Molecularly imprinted polymers for solid-phase extraction of sarcosine as prostate cancer biomarker from human urine. Bulletin of the Korean Chemical Society. 34(8), 2330-2334.


10. Montero L., Conradi S., Weiss H., Popp P., 2005. Determination of phenols in lake and ground water samples by stir bar sorptive extraction–thermal desorption–gas chromatography–mass spectrometry. Journal of Chromatography A. 1071(1), 163-169.


11. Hashemi-Moghaddam H., Rahimian M., Niromand B., 2013. Molecularly Imprinted Polymers for Solid-Phase Extraction of Sarcosine as Prostate Cancer Biomarker from Human Urine. Bull. Korean Chem Soc. 34(8), 2331.


12. Hashemi‐Moghaddam H., Alaeian M.R., 2014. Synthesis of molecularly imprinted polymer for removal of effective impurity (benzhydrol) from diphenhydramine hydrochloride drug. Journal of the Chinese Chemical Society. 61(6), 643-648.


13. Hashemi-Moghaddam H., Abbasi F., 2015. Synthesis of Molecularly Imprinted Polymers Coated on Silica Nanoparticles for Removal of P-Nitrophenol from Crude Pharmaceuticals. Pharmaceutical Chemistry Journal. 49(4), 280-286.


14. Hashemi-Moghaddam H., Jedi D.J., 2015. Solid-phase microextraction of chlorpyrifos in fruit samples by synthesised monolithic molecularly imprinted polymer fibres. International Journal of Environmental Analytical Chemistry. 95(1), 33-44.


15. Wackerlig J., Lieberzeit P.A., 2015. Molecularly imprinted polymer nanoparticles in chemical sensing–Synthesis, characterisation and application. Sensors and Actuators B: Chemical. 207, 144-157.


16. Svec F., Bleha M., Tennikova T. B., Belenkii B.G., 1990. Google Patents.


17. Hashemi-Moghaddam H., Hosseni M., Mohammadhosseini M., 2017. Preparation of molecularly imprinted polymers on the surface of optical fiber for HS-solid-phase microextraction of phenol. Separation Science and Technology. 1-9.


18. Hirayama K., Sakai Y., Kameoka K., Noda K., Naganawa R., 2002. Preparation of a sensor device with specific recognition sites for acetaldehyde by molecular imprinting technique. Sensors and Actuators B: Chemical. 86(1), 20-25.


19. Tang A.N., Duan L., Liu M., Dong X., 2016. An epitope imprinted polymer with affinity for kininogen fragments prepared by metal coordination interaction for cancer biomarker analysis. Journal of Materials Chemistry B. 4(46), 7464-7471.


20. Iida H., Takayanagi K., Nakanishi T., Osaka T., 2007. Synthesis of Fe 3 O 4 nanoparticles with various sizes and magnetic properties by controlled hydrolysis. Journal of Colloid and Interface Science. 314 (1), 274-280.


21. Sun S., Zeng H., 2002. Size-controlled synthesis of magnetite nanoparticles. Journal of the American Chemical Society. 124(28), 8204-8205.


22. Hashemi-Moghaddam H., Ahmadifard M., 2016. Novel molecularly-imprinted solid-phase microextraction fiber coupled with gas chromatography for analysis of furan. Talanta. 150, 148-154.


23. Suedee R., Saelim J., Thavornpibulbut T., Srichana T., 1999. Chiral determination of various adrenergic drugs by thin-layer chromatography using molecularly imprinted chiral stationary phases prepared with α-agonists. Analyst. 124(7), 1003-1009.


24. Suedee R., Songkram C., Petmoreekul A., Sangkunakup S., Sankasa S., Kongyarit N., 1999. Direct enantioseparation of adrenergic drugs via thin-layer chromatography using molecularly imprinted polymers. Journal of Pharmaceutical and Biomedical Analysis. 19(3), 519-527.


25. Fischer L., Mueller R., Ekberg B., Mosbach K., 1991. Direct enantioseparation of. beta.-adrenergic blockers using a chiral stationary phase prepared by molecular imprinting. Journal of the American Chemical Society. 113(24), 9358-9360.


26. Gao F., Hu Y., Chen D., Li-Chan E. C., Grant E., Lu X., 2015. Determination of Sudan I in paprika powder by molecularly imprinted polymers–thin layer chromatography–surface enhanced Raman spectroscopic biosensor. Talanta. 143, 344-352.


27. Chen J.J., Struk K.N., Brennan A.B., 2011. Surface Modification of Silicate Glass Using 3-(Mercaptopropyl) trimethoxysilane for Thiol–Ene Polymerization. Langmuir. 27(22), 13754-13761.


28. García-Galán M.J., Díaz-Cruz M.S., Barceló D., 2008. Identification and determination of metabolites and degradation products of sulfonamide antibiotics. TrAC Trends in Analytical Chemistry. 27(11), 1008-1022.