Enhancing Shelf-life and Quality of Ready-to-Eat Pomegranate Arils with Nanocomposite Film: A PLA/ZnO nanoparticle/Zataria multiflora Essential Oil Innovation
Mojtaba Heydari Majd
1
(
Department of Nutrition, Research Center for Clinical Immunology, Zahedan University of Medical Sciences, Zahedan, Iran
)
Leila Monjazeb Marvdashti
2
(
Food Health Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
)
Amra Bratovcic
3
(
Department of Physical Chemistry and Electrochemistry, Faculty of Technology, University of Tuzla, Tuzla, Bosnia and Herzegovina
)
Anna Abdolshahi
4
(
Food Safety Research Center (salt), School of Nutrition and Food Sciences, Semnan University of Medical Sciences, Semnan, Iran
)
Marzieh Yazdani-Dehnavi
5
(
Department of Food Science and Technology, Faculty of Agriculture, University of Shiraz, Shiraz, Iran
)
fahimeh Ebrahimi Tirtashi
6
(
Department of Food Science and Technology, Faculty of Nutrition Sciences & Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran
)
کلید واژه: Poly(L-lactide), ZnO nanoparticle, Zataria multiflora essential oils, Pomegranate arils, Active packaging ,
چکیده مقاله :
The dried pomegranate (Punica granatum L.) arils have several health benefits and are in demand worldwide. Beside this they are highly perishable due to their high water content and susceptibility to microbial growth, resulting in shortened shelf-life and compromised quality attributes. Therefore, in the current study, the wrapping effects of Poly L-lactide (PLA) activated with zinc oxide nanoparticles (ZnO NPs, 1.5% w/w) and Zataria multiflora Boiss. essential oil (ZEO, 1 and 1.5% w/w) were studied on pomegranate arils' storage-life extension in cold storage. The arils were periodically analyzed for physiochemical properties (weight loss, pH, total soluble solids, color measurement, antioxidant activity, and sensory attributes) and microbial properties (total aerobic bacteria and total fungi counts). Results showed that ZEO caused a decrease in pH during storage, while ZnO caused an increase. The active films were more effective than the control film in maintaining TTS and preventing weight loss of the arils. The PLA/ZnO/1.5% ZEO sample had the highest phenolic content (∼520 mg 100 ml-1 in juice) and antioxidant activity (∼90%) on day 15. However, ZEO had a greater effect on the color properties of the arils compared to ZnO, with 0.5% and 1.5% ZEO-PLA showing the highest a* (55.14) and b* (31.30) values with fresh arils, respectively. The microbial test results showed that ZEO was more effective in controlling bacterial growth than ZnO. Based on atomic absorption spectrophotometer measurement, minimal amounts (∼29 mg Kg-1 sample) of Zn2+ ions were observed to be released into the arils. Overall, the active PLA could be used as a safe and effective way to prolong the storage life of arils in refrigerated conditions.
چکیده انگلیسی :
The dried pomegranate (Punica granatum L.) arils have several health benefits and are in demand worldwide. Beside this they are highly perishable due to their high water content and susceptibility to microbial growth, resulting in shortened shelf-life and compromised quality attributes. Therefore, in the current study, the wrapping effects of Poly L-lactide (PLA) activated with zinc oxide nanoparticles (ZnO NPs, 1.5% w/w) and Zataria multiflora Boiss. essential oil (ZEO, 1 and 1.5% w/w) were studied on pomegranate arils' storage-life extension in cold storage. The arils were periodically analyzed for physiochemical properties (weight loss, pH, total soluble solids, color measurement, antioxidant activity, and sensory attributes) and microbial properties (total aerobic bacteria and total fungi counts). Results showed that ZEO caused a decrease in pH during storage, while ZnO caused an increase. The active films were more effective than the control film in maintaining TTS and preventing weight loss of the arils. The PLA/ZnO/1.5% ZEO sample had the highest phenolic content (∼520 mg 100 ml-1 in juice) and antioxidant activity (∼90%) on day 15. However, ZEO had a greater effect on the color properties of the arils compared to ZnO, with 0.5% and 1.5% ZEO-PLA showing the highest a* (55.14) and b* (31.30) values with fresh arils, respectively. The microbial test results showed that ZEO was more effective in controlling bacterial growth than ZnO. Based on atomic absorption spectrophotometer measurement, minimal amounts (∼29 mg Kg-1 sample) of Zn2+ ions were observed to be released into the arils. Overall, the active PLA could be used as a safe and effective way to prolong the storage life of arils in refrigerated conditions.
1. Melgarejo-Sánchez P., Núñez-Gómez D., Martínez-Nicolás J.J., Hernández F., Legua P., Melgarejo P., 2021. Pomegranate variety and pomegranate plant part, relevance from bioactive point of view: a review. Bioresources and Bioprocessing. 8(1), 1-29.
2. Soloklui A.A.G., Gharaghani A., Oraguzie N., Ramezanian A., 2019. Shelf life and biochemical changes of ready-to-eat arils among nineteen Iranian pomegranate cultivars (Punica granatum L.) during storage. Journal of Food Science and Technology. 56(3), 1416-1426.
3. Adeniyi O.E., Adebayo O.A., Akinloye O., Adaramoye O.A., 2023. Combined cerium and zinc oxide nanoparticles induced hepato-renal damage in rats through oxidative stress mediated inflammation. Scientific Reports. 13(1), 8513.
4. Javedan G., Shidfar F., Davoodi S.H., Ajami M., Gorjipour F., Sureda A., Nabavi S.M., Daglia M., Pazoki‐Toroudi H., 2016. Conjugated linoleic acid rat pretreatment reduces renal damage in ischemia/reperfusion injury: Unraveling antiapoptotic mechanisms and regulation of phosphorylated mammalian target of rapamycin. Molecular Nutrition & Food Research. 60(12), 2665-2677.
5. Ajami M., Eghtesadi S., Razaz J.M., Kalantari N., Habibey R., Nilforoushzadeh M.A., Zarrindast M., Pazoki-Toroudi H., 2011. Expression of Bcl-2 and Bax after hippocampal ischemia in DHA+ EPA treated rats. Neurological Sciences. 32, 811-818.
6. Kohno H., Suzuki R., Yasui Y., Hosokawa M., Miyashita K., Tanaka T., 2004. Pomegranate seed oil rich in conjugated linolenic acid suppresses chemically induced colon carcinogenesis in rats. Cancer Science. 95(6), 481-486.
7. Ali M.Y., Jannat S., Chang M.S., 2023. Discovery of Potent Angiotensin-Converting enzyme inhibitors in Pomegranate as a treatment for hypertension. Journal of Agricultural and Food Chemistry. 71(30), 11476-11490.
8. Pazoki‐Toroudi H.R., Hesami A., Vahidi S., Sahebjam F., Seifi B., Djahanguiri B., 2003. The preventive effect of captopril or enalapril on reperfusion injury of the kidney of rats is independent of angiotensin II AT1 receptors. Fundamental & Clinical Pharmacology. 17(5), 595-598.
9. Pourebrahimi Y., Fazel M., Akhavan H., 2020. Effect of gamma irradiation on the quality characteristics and shelf life of pomegranate arils during refrigerated storage. Journal of Agricultural Science and Technology. 22(3), 723-735.
10. Özdemir K.S., Gökmen V., 2017. Extending the shelf-life of pomegranate arils with chitosan-ascorbic acid coating. LWT-food Science and Technology. 76, 172-180.
11. Abdolshahi A., Heydari Majd M., Abdollahi M., Fatemizadeh S., Monjazeb Marvdashti L., 2020. Edible Film Based on Lallemantia peltata L. Seed Gum: Development and Characterization. Journal of Chemical Health Risks. 12(1), 47-61.
12. Moradinezhad F., Ansarifar E., Moghaddam M. M., 2020. Extending the shelf life and maintaining quality of minimally-processed pomegranate arils using ascorbic acid coating and modified atmosphere packaging. Journal of Food Measurement and Characterization. 14(6), 3445-3454.
13. Salarbashi D., Tafaghodi M., Heydari-Majd M., 2020. Fabrication of curcumin-loaded soluble soy bean polysaccharide/TiO2 bio-nanocomposite for improved antimicrobial activity. Nanomedicine Journal. 7(4), 291-298.
14. Heydari-Majd M., Ghanbarzadeh B., Shahidi-Noghabi M., Najafi M. A., Hosseini M., 2019. A new active nanocomposite film based on PLA/ZnO nanoparticle/essential oils for the preservation of refrigerated Otolithes ruber fillets. Food Packaging and Shelf Life. 19, 94-103.
15. Kamthai S., Magaraphan R., 2019. Development of an active polylactic acid (PLA) packaging film by adding bleached bagasse carboxymethyl cellulose (CMCB) for mango storage life extension. Packaging Technology and Science. 32(2), 103-116.
16. Qin Y., Liu D., Wu Y., Yuan M., Li L., Yang J., 2015. Effect of PLA/PCL/cinnamaldehyde antimicrobial packaging on physicochemical and microbial quality of button mushroom (Agaricus bisporus). Postharvest Biology and Technology. 99, 73-79.
17. Wang B., Sui J., Yu B., Yuan C., Guo L., Abd El-Aty A., Cui B., 2021. Physicochemical properties and antibacterial activity of corn starch-based films incorporated with Zanthoxylum bungeanum essential oil. Carbohydrate Polymers. 254, 117314.
18. da Silva Martins L.H., Neto J.M., Barbosa J.R., Junior G.C.A.C., de Oliveira J.A.R., Rai M., Lopes A.S., 2021. Carboxymethyl cellulose-coated polypropylene films containing essential oil for food preservation Biopolymer-Based Nano Films, Elsevier. pp. 133-147.
19. Heydari-Majd M., Ghanbarzadeh B., Shahidi-Noghabi M., Najafi M.A., Adun P., Ostadrahimid A., 2019. Kinetic release study of zinc from polylactic acid based nanocomposite into food simulants. Polymer Testing. 76, 254-260.
20. AOAC, 2002. Official methods of analysis, 17th ed. Washington, DC: Association of Official Analytical Chemist.
21. Asadi E., Ghehsareh A. M., Moghadam E.G., Hoodaji M., Zabihi H.R., 2019. Improvement of pomegranate colorless arils using iron and zinc fertilization. Journal of Cleaner Production. 234, 392-399.
22. Zhou R., Mo Y., Li Y., Zhao Y., Zhang G., Hu Y., 2008. Quality and internal characteristics of Huanghua pears (Pyrus pyrifolia Nakai, cv. Huanghua) treated with different kinds of coatings during storage. Postharvest Biology and Technology. 49(1), 171-179.
23. Dong H., Cheng L., Tan J., Zheng K., Jiang Y., 2004. Effects of chitosan coating on quality and shelf life of peeled litchi fruit. Journal of Food Engineering. 64(3), 355-358.
24. Zhao J.H., Li H.X., An W., Shi Z.G., Wang Y.J., 2008. Relationship between the Browning and the Radical Oxygenin Postharvest Wolfberry (Lycium L.) Fruits. Acta Botanica Boreali-Occidentalia Sinica. 28(10), 2023-2027.
25. Meng X., Zhang M., Adhikari B., 2014. The effects of ultrasound treatment and nano-zinc oxide coating on the physiological activities of fresh-cut kiwifruit. Food and Bioprocess Technology. 7(1), 126-132.
26. Sogvar O.B., Saba M.K., Emamifar A., Hallaj R., 2016. Influence of nano-ZnO on microbial growth, bioactive content and postharvest quality of strawberries during storage. Innovative Food Science & Emerging Technologies. 35, 168-176.
27. Palma A., Continella A., La Malfa S., Gentile A., D’Aquino S., 2015. Overall quality of ready-to-eat pomegranate arils processed from cold stored fruit. Postharvest Biology and Technology. 109, 1-9.
28. Zahran A., Hassanein R.A., AbdelWahab A.T., 2015. Effect of chitosan on biochemical composition and antioxidant activity of minimally processed ‘Wonderful’pomegranate arils during cold storage. Journal of Applied Botany and Food Quality. 88, 241-248.
29. Ayhan Z., Eştürk O., 2009. Overall quality and shelf life of minimally processed and modified atmosphere packaged “ready‐to‐eat” pomegranate arils. Journal of Food Science. 74(5), C399-C405.
30. Takma D.K., Korel F., 2017. Impact of preharvest and postharvest alginate treatments enriched with vanillin on postharvest decay, biochemical properties, quality and sensory attributes of table grapes. Food Chemistry. 221, 187-195.
31. Emamifar A., Mohammadizadeh M., 2015. Preparation and application of LDPE/ZnO nanocomposites for extending shelf life of fresh strawberries. Food Technology and Biotechnology. 53(4), 488-495.
32. Kapetanakou A., Stragkas I., Skandamis P., 2015. Developing an antimicrobial packaging of ready-to-eat pomegranate arils based on vapors of brandy or distillery ethanol. Food Research International. 69, 141-150.
33. Tehranifar A., Zarei M., Nemati Z., Esfandiyari B., Vazifeshenas M.R., 2010. Investigation of physico-chemical properties and antioxidant activity of twenty Iranian pomegranate (Punica granatum L.) cultivars. Scientia Horticulturae. 126(2), 180-185.
34. Bhatia K., Asrey R., Varghese E., 2015. Correct packaging retained phytochemical, antioxidant properties and increases shelf life of minimally processed pomegranate (Punica granatum L.) arils Cv. Mridula. Journal of Scientific and Industrial Research. 74(3), 141-144.
35. López-Rubira V., Conesa A., Allende A., Artés F., 2005. Shelf life and overall quality of minimally processed pomegranate arils modified atmosphere packaged and treated with UV-C. Postharvest Biology and Technology. 37(2), 174-185.
36. Li W., Li L., Cao Y., Lan T., Chen H., Qin Y., 2017. Effects of PLA film incorporated with ZnO nanoparticle on the quality attributes of fresh-cut apple. Nanomaterials. 7(8), 207.
37. Meighani H., Ghasemnezhad M., Bakshi D., 2014. Evaluation of biochemical composition and enzyme activities in browned arils of pomegranate fruits. International Journal of Horticultural Science and Technology. 1(1), 53-65.
