Investigating the Propagation of Seedless, Seeded and Ornamental Barberry Seedlings through Tissue Culture
محورهای موضوعی :
1 - Department of Agriculture, Damghan Branch, Islamic Azad University, Damghan, Iran
کلید واژه: Barberry, Tissue Culture, Cultivation environment, Ornamental,
چکیده مقاله :
In order to investigate the reproduction of seedless, seeded and ornamental barberry shrubs through tissue culture, a factorial experiment in the form of a completely randomized design in three replications and with three factors, the first factor of three types of barberry shrubs (seedless, seeded and ornamental), the second factor of BAP hormone at four levels (0, 1, 2, 3 mg L-1) and the third factor of IBA hormone at two levels (0 and 0.25 mg L-1) was done in the laboratory of Islamic Azad University, Damghan branch in 2019. The culture medium used was based on Murashige and Stooge (MS). After preparing the culture medium and sterilizing it by autoclave, it was distributed in the test tubes. After preparation, the samples were cultured in a sterile environment and then the samples were stimulated to grow using growth hormones. The investigated traits included germination, number of leaves, length, width and surface of leaves, ratio of width to length of leaves, fresh weight and dry weight of leaves. After collecting information, the data obtained from the experiment was organized and then analysis of variance was performed with SAS statistical software and comparison of means with Duncan's multi-range test. Pearson's correlation coefficient was used in order to determine the relationships of traits. The main effects and some interaction effects on the time of greening, number of leaves, length, width, leaf area and leaf width to length ratio, leaf fresh weight and dry weight were significant. The correlation coefficient shown between the dry weight of leaves with the number of leaves (r=0.88**), leaf length (r=0.74**), leaf width (r=0.74**), leaf area (r=0.75**), green leaf weight(r=0.96**) positive correlation was significant at 1% probability level.
In order to investigate the reproduction of seedless, seeded and ornamental barberry shrubs through tissue culture, a factorial experiment in the form of a completely randomized design in three replications and with three factors, the first factor of three types of barberry shrubs (seedless, seeded and ornamental), the second factor of BAP hormone at four levels (0, 1, 2, 3 mg L-1) and the third factor of IBA hormone at two levels (0 and 0.25 mg L-1) was done in the laboratory of Islamic Azad University, Damghan branch in 2019. The culture medium used was based on Murashige and Stooge (MS). After preparing the culture medium and sterilizing it by autoclave, it was distributed in the test tubes. After preparation, the samples were cultured in a sterile environment and then the samples were stimulated to grow using growth hormones. The investigated traits included germination, number of leaves, length, width and surface of leaves, ratio of width to length of leaves, fresh weight and dry weight of leaves. After collecting information, the data obtained from the experiment was organized and then analysis of variance was performed with SAS statistical software and comparison of means with Duncan's multi-range test. Pearson's correlation coefficient was used in order to determine the relationships of traits. The main effects and some interaction effects on the time of greening, number of leaves, length, width, leaf area and leaf width to length ratio, leaf fresh weight and dry weight were significant. The correlation coefficient shown between the dry weight of leaves with the number of leaves (r=0.88**), leaf length (r=0.74**), leaf width (r=0.74**), leaf area (r=0.75**), green leaf weight(r=0.96**) positive correlation was significant at 1% probability level.
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