A Cytometric Analysis in Circulating Blood Lymphocytes of Water pipe Tobacco Smokers and Non-Smokers by Cytokinesis-block Micronucleus Technique: A Genomic Health Study on Apparent Healthy Premenopausal Women in Tehran
محورهای موضوعی :Negar Bahrami 1 , Sepideh Arbabi Bidgoli 2 , Ramin Abrashami 3 , Aziz Mahmoudzadeh 4 , Masoumeh Heshmati 5
1 - Department of Pharmacology and Toxicology, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
2 - Department of Pharmacology and Toxicology, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
3 - Department of Clinical Pharmacy, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
4 - Faculty of Passive Defense, Malek Ashtar University of Technology, Iran
5 - Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
کلید واژه: Waterpipe smoking, MNi frequency, Genomic health, Tehran,
چکیده مقاله :
According to the association between increased micronuclei (MNi) level in reproductive age women and increased risk of congenital abnormalities, we aimed in this study to find the contribution of WTS to women's health in apparently healthy young cases by comparing the variability of MN values in water pipe smokers and non-smokers. Finding the associations between MNi levels and demographic, socioeconomic, reproductive, and lifestyle factors was the secondary scope of this study. This cross-sectional case-control study was performed on 30 premenopausal women (15 cases and 15 controls) who lived in Tehran, according to our inclusion criteria. The mean MN frequency among waterpipe smokers was 28.53± 7.462, whereas the same parameter in the control group was 6.53 + 3.24 (p= 0.001). Non-parametric tests revealed a significant association between MNi and frequency of waterpipe smoking (p = 0.001), age of starting waterpipe smoking (p = 0.003), concurrent use of alcoholic drinks (p= 0.004), and secondhand smoking (p =0.001). In the context of heavy environmental pollution in Tehran, significantly higher MNi frequencies and decreased genomic health in waterpipe smoker women in reproductive age may predispose them to an increased risk of harmful reproductive outcomes. These findings emerge from governmental and non-governmental biomonitoring programs in high-risk women to concern more on unhealthy lifestyles and environmental pollution.
According to the association between increased micronuclei (MNi) level in reproductive age women and increased risk of congenital abnormalities, we aimed in this study to find the contribution of WTS to women's health in apparently healthy young cases by comparing the variability of MN values in water pipe smokers and non-smokers. Finding the associations between MNi levels and demographic, socioeconomic, reproductive, and lifestyle factors was the secondary scope of this study. This cross-sectional case-control study was performed on 30 premenopausal women (15 cases and 15 controls) who lived in Tehran, according to our inclusion criteria. The mean MN frequency among waterpipe smokers was 28.53± 7.462, whereas the same parameter in the control group was 6.53 + 3.24 (p= 0.001). Non-parametric tests revealed a significant association between MNi and frequency of waterpipe smoking (p = 0.001), age of starting waterpipe smoking (p = 0.003), concurrent use of alcoholic drinks (p= 0.004), and secondhand smoking (p =0.001). In the context of heavy environmental pollution in Tehran, significantly higher MNi frequencies and decreased genomic health in waterpipe smoker women in reproductive age may predispose them to an increased risk of harmful reproductive outcomes. These findings emerge from governmental and non-governmental biomonitoring programs in high-risk women to concern more on unhealthy lifestyles and environmental pollution.
1. Hirpa S., Fogarty A., Addissie A., Bauld L., Frese T., Unverzagt S., Kantelhardt EJ., Getachew S., Deressa W., 2021. An Emerging Problem of Shisha Smoking among High School Students in Ethiopia. Int J Environ Res Public Health. 18(13), 7023.
2. El-Shahawy O., Labib K., Stevens E., Kahn LG., Anwar W., Oncken C., Loney T., Sherman SE., Mead-Morse EL., 2021. Exclusive and Dual Cigarette and Hookah Smoking Is Associated with Adverse Perinatal Outcomes among Pregnant Women in Cairo, Egypt. Int J Environ Res Public Health. 18(24), 12974.
3. Al-Sawalha N.A., Almomani B.A., Al-Shatnawi S.F., Almomani B.N., 2021. Attitudes and Knowledge of the Harmful Effects of Waterpipe Tobacco Smoking among university students: A study from Jordan. Environ Sci Pollut Res Int. 28(32), 43725-43731.
4. Qasim H., Alarabi A.B., Alzoubi K.H., Karim Z.A., Alshbool F.Z., Khasawneh F.T., 2019. The effects of hookah/waterpipe smoking on general health and the cardiovascular system. Environ Health Prev Med. 24(1), 58.
5. Stroud L., Werner E., Matteson K., Carey M., Helen G.S., Eissenberg T., Scott-Sheldon L.A.J., 2020. Waterpipe (hookah) tobacco use in pregnancy: use, preferences and perceptions of flavours. Tob Control. 29(Suppl 2), s62-s71.
6. Kheirallah K.A., Shugaa Addin N., Alolimat M.M., 2021. Trends of maternal waterpipe, cigarettes, and dual tobacco smoking in Jordan. A decade of lost opportunities. PLoS ONE. 16(7), e0253655
7. Münzel T., Hahad O., Kuntic M., Keaney J.F., Deanfield J.E., Daiber A., 2020. Effects of tobacco cigarettes, e-cigarettes, and waterpipe smoking on endothelial function and clinical outcomes. Eur Heart J. 41(41), 4057-4070.
8. Patil S., Awan K.H., Arakeri G., Aljabab A., Ferrari M., Gomes C.C., Gomez R.S., Brennan P.A., 2019. The relationship of "shisha" (water pipe) smoking to the risk of head and neck cancer. J Oral Pathol Med. 48(4), 278-283.
9. Qasim H., Alarabi A.B., Alzoubi K.H., Karim Z.A., Alshbool F.Z., Khasawneh F.T., 2019. The effects of hookah/waterpipe smoking on general health and the cardiovascular system. Environ Health Prev Med. 24(1), 58.
10. Saffar Soflaei S., Darroudi S., Tayefi M., Nosrati Tirkani A., Moohebati M., Ebrahimi M., Esmaily H., Parizadeh SMR., Heidari-Bakavoli A.R., Ferns G.A., Ghayour-Mobarhan M., 2018. Hookah smoking is strongly associated with diabetes mellitus, metabolic syndrome and obesity: a population-based study. Diabetol Metab Syndr. 10(33), 1-6.
11. Badran M., Laher I., 2020. Waterpipe (shisha, hookah) smoking, oxidative stress and hidden disease potential. Redox Biol. 34, 101455.
12. Xiong Y., Xi S., Gara SK., Shan J., Gao J., Zhang M., Shukla V., Wang R., Hoang C.D., Chen H., Schrump D.S., 2021. Hookah Smoke Mediates Cancer-Associated Epigenomic and Transcriptomic Signatures in Human Respiratory Epithelial Cells. JTO Clin Res Rep. 2(7), 100181.
13. Kadhum M., Sweidan A., Jaffery A.E., Al-Saadi A., Madden B., 2015. A review of the health effects of smoking shisha. Clin Med (Lond). 15(3), 263-6.
14. Zaid K., Azar-Maalouf E., Barakat C., Chantiri M., 2018. Overexpression in Oral Mucosa in Relation to Shisha Smoking in Syria and Lebanon. Asian Pac J Cancer Prev. 19(7), 1879-1882.
15. Ramôa C.P., Eissenberg T., Sahingur S.E., 2017. Increasing popularity of waterpipe tobacco smoking and electronic cigarette use: Implications for oral healthcare. J Periodontal Res. 52(5), 813-823.
16. Silva-Grecco R.L., Navarro G.C., Cruz R.M., Balarin M.A., 2012. Micronucleated lymphocytes in parents of Down syndrome children. Braz J Med Biol Res. 45(7), 573-7.
17. Bolognesi C., Fenech M., 2013. Micronucleus assay in human cells: lymphocytes and buccal cells. Methods Mol Biol. 1044, 191-207.
18. Fenech M., 2020. Cytokinesis-Block Micronucleus Cytome Assay Evolution into a More Comprehensive Method to Measure Chromosomal Instability. Genes (Basel). 11(10), 1203.
19. Beyls E., Baeyens A., Vral A., 2021. The cytokinesis-block micronucleus assay for cryopreserved whole blood. Int J Radiat Biol. 97(9), 1252-1260.
20. Shen X., Chen Y., Li C., Yang F., Wen Z., Zheng J., Zhou Z., 2022. Rapid and automatic detection of micronuclei in binucleated lymphocytes image. Sci Rep. 12(1), 3913.
21. Fielder R.L., Carey K.B., Carey M.P., 2012. Prevalence, frequency, and initiation of hookah tobacco smoking among first-year female college students: a one-year longitudinal study. Addict Behav. 37(2), 221-4.
22. Stroud L.R., Bublitz M.H., Crespo F.A., Lester B., Salisbury A.L., 2020. Maternal smoking in pregnancy, fetal activity & newborn behavioral state: An observational ultrasound study. Neurotoxicol Teratol. 81, 106894.
23. Valenzuela F.J., Pérez-Sepúlveda A., Torres M.J., Correa P., Repetto G.M., Illanes S.E., 2012. Pathogenesis of preeclampsia: the genetic component. J Pregnancy. 2012, 632732.
24. Silva-Grecco R.L., Navarro G.C., Cruz R.M., Balarin M.A., 2012. Micronucleated lymphocytes in parents of Down syndrome children. Braz J Med Biol Res. 45(7), 573-7.
25. Andreassi M.G., Barale R., Iozzo P., Picano E., 2011. The association of micronucleus frequency with obesity, diabetes and cardiovascular disease. Mutagenesis. 26(1), 7-83.
26. Rastkhah E., Zakeri F., Ghoranneviss M., Rajabpour M.R., Farshidpour M.R., Mianji F., Bayat M., 2016. The cytokinesis-blocked micronucleus assay: dose-response calibration curve, background frequency in the population and dose estimation. Radiat Environ Biophys. 55(1), 41-51.
27. Durmaz B., Taslidere H., Koturoglu G., Gunduz C., Orman M., Cogulu O., 2019. Determination of Lymphocyte Cytokinesis-Block Micronucleus Values in Apparently Healthy Children by means of Age and Sex. Biomed Res Int. 2019, 8729561.
28. Gajski, G., Gerić M., Pehnec, G., Matković, K., Rinkovec, J., Jakovljević, I., Godec, R., Žužul, S., Bešlić, I., Cvitković, A., 2022. Associating Air Pollution with Cytokinesis-Block Micronucleus Assay Parameters in Lymphocytes of the General Population in Zagreb (Croatia). Int J Mol Sci. 23, 10083.
29. Dehghan A., Khanjani N., Bahrampour A., Goudarzi G., Yunesian M., 2018. The relation between air pollution and respiratory deaths in Tehran, Iran- using generalized additive models. BMC Pulm Med. 18(1), 49.
30. Bayat R., Ashrafi K., Shafiepour Motlagh M., Hassanvand MS., Daroudi R., Fink G., Künzli N., 2019. Health impact and related cost of ambient air pollution in Tehran. Environ Res. 176, 108547.
31. Effati-Daryani F., Zarei S., Mohammadi A., Hemmati E., Ghasemi Yngyknd S., Mirghafourvand M., 2020. Depression, stress, anxiety and their predictors in Iranian pregnant women during the outbreak of COVID-19. BMC Psychol. 8(1), 99.
32. Benassi-Evans B., Fenech M., 2011. Chronic alcohol exposure induces genome damage measured using the cytokinesis-block micronucleus cytome assay and aneuploidy in human B lymphoblastoid cell lines. Mutagenesis. 26(3), 421-9.
33. Husgafvel-Pursiainen K., 2004. Genotoxicity of environmental tobacco smoke: a review. Mutat Res. 567(2-3), 427-45.
