Anti-biofilm Effect of Lactic Acid Bacteria Isolated from Abali Local Buttermilk against Urinary Enterococcus faecalis
محورهای موضوعی :
Zahra Golshahi
1
,
Hossein Abbaspour
2
,
Nazila Arbab Soleimani
3
1 - Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran
2 - Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran
3 - Department of Microbiology, Damghan Branch, Islamic Azad University, Damghan, Iran
کلید واژه: Lactic acid bacteria, Enterococcus faecalis, Traditional buttermilk, Anti-biofilm effect,
چکیده مقاله :
Enterococcus faecalis is a biofilm-producing bacterium and the cause of urinary tract infection. This bacterium is resistant to many antibiotics today and new methods such as use of lactic acid bacteria are needed. The present study aimed to isolate lactic acid bacteria with antagonistic and anti-biofilm effects against E. faecalis as a candidate for new treatment. In order to study of the anti -biofilm effect of lactic acid bacteria against E. faecalis, 30 samples of urinary E. faecalis were collected and their biofilm formation was investigated by a 96-well microtiter plate method. 10 samples of Abali traditional buttermilk (Dough) were collected and lactic acid bacteria were isolated and purified. Biochemical tests were done for the identification of lactic acid bacteria. The antagonistic effect of lactic acid bacteria supernatant against E. faecalis was investigated by determining the minimum growth inhibitory and lethal concentration in the 96-well microtiter plate. The anti-biofilm effect of the isolates was investigated by the 96-well microtiter plate method. The best isolates were molecularly identified in terms of anti-biofilm properties by the ribotyping method. Out of 30 E. faecalis bacteria samples, 12 samples (40%), 10 samples (33.33%), and 7 samples (23.33%) produced strong, moderate, and weak biofilm, respectively. 1 sample (3.33) was unable to form biofilm. From traditional buttermilk samples, 9 lactic isolates were isolated, all of which had antagonistic and anti-biofilm effects. According to molecular identification, D4 (Lactobacillus fermentum) was identified with the highest anti-biofilm effect against E. faecalis. Based on the results of this research, the lactic isolate (L. fermentum) with antagonistic and anti-biofilm properties can be a candidate for the treatment of urinary infections caused by E. faecalis in the future.
Enterococcus faecalis is a biofilm-producing bacterium and the cause of urinary tract infection. This bacterium is resistant to many antibiotics today and new methods such as use of lactic acid bacteria are needed. The present study aimed to isolate lactic acid bacteria with antagonistic and anti-biofilm effects against E. faecalis as a candidate for new treatment. In order to study of the anti -biofilm effect of lactic acid bacteria against E. faecalis, 30 samples of urinary E. faecalis were collected and their biofilm formation was investigated by a 96-well microtiter plate method. 10 samples of Abali traditional buttermilk (Dough) were collected and lactic acid bacteria were isolated and purified. Biochemical tests were done for the identification of lactic acid bacteria. The antagonistic effect of lactic acid bacteria supernatant against E. faecalis was investigated by determining the minimum growth inhibitory and lethal concentration in the 96-well microtiter plate. The anti-biofilm effect of the isolates was investigated by the 96-well microtiter plate method. The best isolates were molecularly identified in terms of anti-biofilm properties by the ribotyping method. Out of 30 E. faecalis bacteria samples, 12 samples (40%), 10 samples (33.33%), and 7 samples (23.33%) produced strong, moderate, and weak biofilm, respectively. 1 sample (3.33) was unable to form biofilm. From traditional buttermilk samples, 9 lactic isolates were isolated, all of which had antagonistic and anti-biofilm effects. According to molecular identification, D4 (Lactobacillus fermentum) was identified with the highest anti-biofilm effect against E. faecalis. Based on the results of this research, the lactic isolate (L. fermentum) with antagonistic and anti-biofilm properties can be a candidate for the treatment of urinary infections caused by E. faecalis in the future.
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