Relationship of Sodium Nitroprusside with Growth and Antioxidant Enzymes of Canola under Lead Stress
محورهای موضوعی :
Hossein Hamidi
1
(
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
)
Nahid Masoudian
2
(
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
)
Mostafa Ebadi
3
(
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
)
Bostan Roudi
4
(
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
)
کلید واژه: Chlorophyll, Lead, weight, Sodium nitroprusside, Antioxidant enzymes,
چکیده مقاله :
Lead is a toxic heavy-metal pollutant which is hazardous to human health and the environment. Sodium nitroprusside is commonly used as a nitric oxide donor in plants. Nitric oxide is a bioactive molecule playing an important role in response to stress in plants. Weight, chlorophyll content, and the activity of catalase (EC 1.11.1. 6) and peroxidase (EC 1.11.1. 7) antioxidant enzymes of canola (Brassica napus L.) Hyola 401 in lead stress were investigated. This study tested the hypothesis that sodium nitroprusside plays an ameliorating role under lead-toxicity in canola. For seven days, thirteen-day plants were exposed to two levels of sodium nitroprusside (0 and 100 µM) and three levels of lead (0, 100, and 200 µM). Dry and fresh weight and chlorophyll content were decreased in lead stress, while sodium nitroprusside treatment increased weight and chlorophyll b in the same conditions. Lead stress increased the activity of antioxidant enzymes, and sodium nitroprusside treatment reduced their activity. The results showed that the use of sodium nitroprusside reduces lead toxicity.
1. Rakow G., 2004. Species Origin and Economic Importance of Brassica. In Brassica; Pua E-C, Douglas CJ. Eds.; Springer Berlin Heidelberg: Berlin, Germany. 54, 3-11.
2. Hemmat A., 2009. Reduction in Primary Tillage Depth and Secondary Tillage Intensity for Irrigated Canola Production in a Loam Soil in Central Iran. Journal of Agricultural Science and Technology. 11(3), 275-288.
3. Rahimi S., Kamran Azad S., Karimi Torshizi M.A., 2011. Omega-3 Enrichment of Broiler Meat by Using Two Oilseeds. Journal of Agricultural Science and Technology. 13(3), 353-365.
4. Kowalska J., 2014. Organically grown Brassica napus – Use of border strips and Trichoderma. Acta Agriculturae Scandinavica, Section B — Soil & Plant Science. 64(6), 529-536.
5. Ali B., Mwamba T.M., Gill R.A., Yang C., Ali S., Daud M.K., Wu Y., Zhou W., 2014. Improvement of element uptake and antioxidative defense in Brassica napus under lead stress by application of hydrogen sulfide. Plant Growth Regulation. 74(3), 261-273.
6. Taghizadeh M., Kafi M., Fattahi Moghadam M.R., 2015. Breeding by In vitro Culture to Improve Tolerance and Accumulation of Lead in Cynodon Dactylon L. Journal of Agricultural Science and Technology. 17(20), 1851-1860.
7. Gubrelay U., Agnihotri R.K., Shrotriya S., Sharma R., 2015. Effect of Lead stress on phosphatase activity and reducing power assay of Triticum aestivum. Cellular and molecular biology.61(3), 57-62.
8. He Z.L., Yang X.E., Stoffella P.J., 2005. Trace elements in agroecosystems and impacts on the environment.Journal of Trace Elements in Medicine and Biology. 19(2-3), 125-140.
9. Reddy A.M., Kumar S.G., Jyonthsnakumari G., Thimmanaik S., Sudhakar C., 2005. Lead induced changes in antioxidant metabolism of horsegram (Macrotyloma uniflorum (Lam.) Verdc.) and bengalgram (Cicer arietinum L.). Chemosphere. 60(1), 97-104.
10. Ruley A.T., Sharma N.C., Sahi S.V., 2004. Antioxidant defense in a lead accumulating plant, Sesbania drummondii. Plant Physiology and Biochemistry. 42(11), 899-906.
11. Verma S., Dubey R.S., 2003. Lead toxicity induces lipid peroxidation and alters the activities of antioxidant enzymes in growing rice plants. Plant Science. 164(4), 645-655.
12. Ghorbani H., Hafezi Moghads N., Kashi H., 2015. Effects of Land Use on the Concentrations of Some Heavy Metals in Soils of Golestan Province, Iran. Journal of Agricultural Science and Technology. 17(4), 1025-1040.
13. Thakur S., Singh L., Zularisam A.W., Sakinah M., Din M.F.M., 2017. Lead induced oxidative stress and alteration in the activities of antioxidative enzymes in rice shoots. Biologia Plantarum.61(3), 595-598.
14. Adriano D.C., 2001. Trace Elements in Terrestrial Environments: Biogeochemistry, Bioavailability, and Risks of Metals, 2nd edition. Springer, New York.
15. Krishnaraj S., Dan T.V., Saxena P.K., 2000. A Fragrant Solution to Soil Remediation. International Journal of Phytoremediation. 2(2), 117-132.
16. Macfarlane G.R., 2003. Chlorophyll a Fluorescence as a Potential Biomarker of Zinc Stress in the Grey Mangrove, Avicennia marina (Forsk.) Vierh. Bulletin of Environmental Contamination and Toxicology. 70(1), 90-96.
17. Maksymiec W., Wójcik M., Krupa Z., 2007. Variation in oxidative stress and photochemical activity in Arabidopsis thaliana leaves subjected to cadmium and excess copper in the presence or absence of jasmonate and ascorbate. Chemosphere. 66(3), 421-427.
18. Küpper H., Küpper F., Spiller M., 1996. Environmental relevance of heavy metal-substituted chlorophylls using the example of water plants. Journal of Experimental Botany. 47(2), 259-266.
19. Shah K., Kumar R.G., Verma S., Dubey R.S., 2001. Effect of cadmium on lipid peroxidation, superoxide anion generation and activities of antioxidant enzymes in growing rice seedlings. Plant Science. 161(6), 1135-1144.
20. Mittler R., 2002. Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science. 7(9), 405-410.
21. Geebelen W., Vangronsveld J., Adriano D.C., Van Poucke L.C., Clijsters H., 2002. Effects of Pb-EDTA and EDTA on oxidative stress reactions and mineral uptake in Phaseolus vulgaris. Physiologia Plantarum. 115(3), 377-384.
22. Malar S., Manikandan R., Favas P.J.C., Sahi S.V., Venkatachalam P., 2014. Effect of lead on phytotoxicity, growth, biochemical alterations and its role on genomic template stability in Sesbania grandiflora: A potential plant for phytoremediation. Ecotoxicology and Environmental Safety. 108, 249-257.
23. Qiao W., Li C., Fan L.M., 2014. Cross-talk between nitric oxide and hydrogen peroxide in plant responses to abiotic stresses. Environmental and Experimental Botany. 100, 84-93.
24. Lopez-Carrion A.I., Castellano R., Rosales M.A., Ruiz J.M., Romero L., 2008. Role of nitric oxide under saline stress: implications on proline metabolism. Biologia Plantarum. 52(3), 587-591.
25. Arasimowicz M., Floryszak-Wieczorek J., 2007. Nitric oxide as a bioactive signalling molecule in plant stress responses. Plant Science. 172(5), 876-887.
26. Xiong J., Fu G., Tao L., Zhu C., 2010. Roles of nitric oxide in alleviating heavy metal toxicity in plants. Archives of Biochemistry and Biophysics. 497(1-2), 13-20.
27. Phang I.C., Leung D.W.M., Taylor H.H., Burritt D.J., 2011. The protective effect of sodium nitroprusside (SNP) treatment on Arabidopsis thaliana seedlings exposed to toxic level of Pb is not linked to avoidance of Pb uptake. Ecotoxicology and Environmental Safety. 74(5), 1310-1315.
28. Veeramani K., Avudainayagam S., Doraisamy P., Chandrasekharan C.N., 2012. Chemical Immobilization of Lead (Pb) in Long Term Sewage Irrigated Soil. Journal of Agricultural Science and Technology. 14(2), 449-458.
29. Kaur G., Singh H.P., Batish D.R., Mahajan P., Kohli R.K., Rishi V., 2015. Exogenous nitric oxide (NO) interferes with lead (Pb)-induced toxicity by detoxifying reactive oxygen species in hydroponically grown wheat (Triticum aestivum) roots. PLoS One. 10(9): e0138713.
30. Jensen A., 1978. Chlorophylls and carotenoids. In Handbook of Phycological Methods: Physiological and Biochemical Methods (Hellebust J.A., Craigie J.S., editors), Cambridge University Press, Cambridge, 59-70.
31. Koroi S.A., 1989. Gel electrophoresis tissue and spectrophotometrscho unter uchungen zomeinfiuss der temperature auf struktur der amylase and peroxidase isoenzyme. Physiology Review. 20, 15-23.
32. Chance B., Maehly A.C., 1955. Assay of Catalase and Peroxidase. Methods in Enzymology. 2, 764-775.
33. Sun Y., 2017. Ecological Risk Evaluation of Heavy Metal Pollution in Soil Based on Simulation. Polish Journal of Environmental Studies. 26(4), 1693-1699.
34. Hang X.S., Wang H.Y., Zhou J.M., 2010. Soil heavy-metal distribution and transference to soybeans surrounding an electroplating factory. Acta Agriculturae Scandinavica, Section B — Soil & Plant Science. 60(2), 144-151.
35. Shahid M., Dumat C., Silvestre J., Pinelli E., 2012. Effect of fulvic acids on lead-induced oxidative stress to metal sensitive Vicia faba L. plant. Biology and Fertility of Soils. 48(6), 689-697.
36. Santolini J., Andre F., Jeandrozb S., Wendehenneb D., 2017. Nitric oxide synthase in plants: Where do we stand? Nitric Oxide. 63, 30-38.
37. Beligni M.V., Lamattina L., 1999. Is nitric oxide toxic or protective? Trends in Plant Science. 4(8), 299-300.
38. Emamverdian A., Ding Y., 2017. HMs Induced Changes on Growth, Antioxidant Enzyme’s Activity, gas Exchange Parameters and Protein Structures in Sasa Kongosanensis f. Aureo – Striatus. Polish Journal of Environmental Studies. 26(2), 585-592.
39. Desikan R., Cheung M.K., Bright J., Henson D., Hancock J.T., Neill S.J., 2004. ABA, hydrogen peroxide and nitric oxide signalling in stomatal guard cells. Journal of Experimental Botany. 55(395), 205-212.
40. Kaur L., Gadgil K., Sharma S., 2018. Lead and nickel accumulation in Brassica juncea arawali growing in contaminated soil. Jornal of Chemical Health Risks. 8(2), 157-175.
41. Mahanty H.K., McWha J.A., 1976. Sensitivity of Spirodela oligorrhiza (Kurz) Hegelm. to a polychlorinated biphenyl (Aroclor 1242). New Zealand Journal of Botany. 14(1), 9-12.
42. Sadeghipour O., 2016. Pretreatment with nitric oxide reduces lead toxicity in cowpea (Vigna unguiculata [L.] Walp.). Archives of Biological Sciences. 68(1), 165-175.
43. Graziano M., Beligni M.V., Lamattina L., 2002. Nitric Oxide Improves Internal Iron Availability in Plants. Plant Physiology. 130, 1852-1859.
44. Graziano M., Lamattina L., 2005. Nitric oxide and iron in plants: an emerging and converging story. Trends in Plant Science. 10(1), 4-8.
45. Mishra S., Srivastava S., Tripathi R., Kumar R., Seth C., Gupta D., 2006. Lead detoxification by coontail (Ceratophyllum demersum L.) involves induction of phytochelatins and antioxidant system in response to its accumulation. Chemosphere. 65(6), 1027-1039.
46. Parmar N.G., Vithalani S.D., Chanda S.V., 2002. Alteration in growth and peroxidase activity by heavy metals in Phaseolus seedlings. Acta Physiologiae Plantarum. 24(1), 89-95.
47. Sedghi M., Seyed Sharifi R., Pirzad A.R., Amanpour-Balaneji B., 2012. Phytohormonal Regulation of Antioxidant Systems in Petals of Drought Stressed Pot Marigold (Calendula officinalis L.). Journal of Agricultural Science and Technology. 14(4), 869-878.
48. Ali B., Deng X., Hu X., Gill R.A., Ali S., Wang S., Zhou W., 2015. Deteriorative Effects of Cadmium Stress on Antioxidant System and Cellular Structure in Germinating Seeds of Brassica napus L. Journal of Agricultural Science and Technology. 17(1), 63-74.
49. Singh H.P., Batish D.R., Kaur G., Arora K., Kohli R.K., 2008. Nitric oxide (as sodium nitroprusside) supplementation ameliorates Cd toxicity in hydroponically grown wheat roots. Environmental and Experimental Botany. 63(1-3), 158-167.
50. Wang Y.S., Yang Z.M., 2005. Nitric Oxide Reduces Aluminum Toxicity by Preventing Oxidative Stress in the Roots of Cassia tora L. Plant and Cell Physiology. 46(12), 1915-1923.
51. Akram N.A., Iqbal M., Muhammad A., Ashraf M., Al-Qurainy F., Shafiq S., 2018. Aminolevulinic acid and nitric oxide regulate oxidative defense and secondary metabolisms in canola (Brassica napus L.) under drought stress. Protoplasma. 255(1), 163-174.
