Cellular Toxicity of Multi-walled Carbon Nanotubes on Human Lung Cells
محورهای موضوعی :Nafiseh Nasirzadeh 1 , Yahya Rasoulzadeh 2 , Mansour Rezazadeh Azari 3 , Yousef Mohammadian 4
1 - Department of Occupational Health Engineering, Faculty of Health, Tabriz University of Medical Sciences, Tabriz, Iran
2 - Department of Occupational Health Engineering, Faculty of Health, Tabriz University of Medical Sciences, Tabriz, Iran
3 - School of Public Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4 - Department of Occupational Health Engineering, Faculty of Health, Tabriz University of Medical Sciences, Tabriz, Iran
کلید واژه: Carbon nanotubes, Cytotoxicity, IC50, NOAEC, A549 cells,
چکیده مقاله :
Nowadays, multi-walled carbon nanotubes (MWCNTs) are used in various industries. Considering the exposure probability of these nanomaterials to humans, the purpose of the present study is to assess the effect of MWCNTs on cellular toxicity of human alveolar epithelial. The A549 cells were cultured and treated to various doses of MWCNTs at three different times. Finally, the Tetrazolium colorimetric (MTT) assay was implemented for evaluating the cellular viability. The results indicated that the cytotoxicity for MWCNTs on the human alveolar epithelial cells is related to dose and time of exposure. The inhibitory concentration of 50% (IC50) and non-observed adverse effect concentration (NOAEC) are calculated to be 103.6 as well as 0.65μg/mL, respectively. The findings of this present study could contribute to a better understanding of MWCNTs substances and might be useful as a basis for the future risk evaluation studies of exposed population in industries.
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