Acute Genotoxic Effects of Effluent Water of Thermo-Power Plant “Kosova†In Tradescantia Pallida
محورهای موضوعی :I.R. Elezaj 1 , L.B. Millaku 2 , R.H. Imeri-Millaku 3 , Q.I. Selimi 4 , K. Rr. Letaj 5
1 - Department of Biology, University of Prishtina,
P.O.Box 10.000 Prishtinë, Republic of Kosovo
2 - Department of Biology, University of Prishtina,
P.O.Box 10.000 Prishtinë, Republic of Kosovo
3 - Department of Biology, University of Prishtina,
P.O.Box 10.000 Prishtinë, Republic of Kosovo
4 - Department of Biology, University of Prishtina,
P.O.Box 10.000 Prishtinë, Republic of Kosovo
5 - Department of Biology, University of Prishtina,
P.O.Box 10.000 Prishtinë, Republic of Kosovo
کلید واژه: Tradescantia, MN-test, cell aberration, mitotic index,
چکیده مقاله :
The aim of this study was the evaluation of acute genotoxic effect of effluent water of thermo-power plant by means of Tradescantia root tips micronucleus test (MN), mitotic index and cell aberrations.  Tradescantia, was experimentally treated (for 24 h), with effluent water of thermo-power plant in different dilution ratios (negative control – distilled water; primary untreated effluent water and 1:1; 1:2; 1:3; 1:4; 1:5; 1:6 and 1:7 respectively). Number of aberrant cells, and frequency of micronuclei (MN), in meristematic root tip cells of treated plants (Tradescantia), were significantly increased (P<0.001; P<0.001 respectively), while the mitotic index in all treated plants was progressively decreased in comparison to the negative control. The results of present study indicate that Tradescantia root-tip micronucleus assay with direct exposure of intact plants is an appropriate method which enables to detect genotoxic effects of effluent waters.
- De Rainho,C.R., A. Kaezner, C.A.F.Aiub, and I.
- Felzenszwalb.2010. Ability of Alium cepa L.
- root tips and Tradescantia pallida var.
- puerpurea in N-nitrosodiethylamine
- genotoxicity and mutagenicity evaluation. An
- Acad Bras Cience. 82, (4), 921-932.
- DeMarco A., S. Paglialunga, M. Rizzoni, A. Testa
- and S. Trinca. 1988. Induction of micronuclei
- in Vicia faba roots tips treated with heavy
- metals (cadmium and chromium) in the
- presence of NTA. Mutat. Res. 206, 311-315.
- Eckel, P.M. 1995. Aquatic genotoxicity testing with
- rat hepatocytes in primary culture II. Induction
- of micronuclei and chromosomal aberrations.
- Sci Total Environ. 159, 81-89.
- Egito L. C. M., M.G. Medeiros, S. R. B. Medeiros,
- and L.F. Agez-Lima. 2007. Cytotoxic and
- genotoxic potential of surface water from the
- Pitimbu river, northeastern /RN Brazil.
- Genetics and Molecular Biology, 30 (2), 435-
- Elezaj, I.R., K.H.Kurteshi, Q.I.Selimi, and
- K.Rr.Letaj. 2003. Genotoxic effects of Sitnica
- river (polluted from effluent waters of
- ââÅKosovaââ Power plant) in three fish species
- (Barbus barbus, Perca fluviatilis and
- Oncorhynchus muciss Walbaum). FASEB.
- A1368/ 886.38.
- Haider, T., R. Sommer, S. Knasmuller, P. Eckel,
- W. Pribil, A. Cabaj and M. Kundi.2002.
- Genotoxic response of Austrian groundwater
- samples treated under standardized UV (254
- nm)- disinfection conditions in a comb ination
- of three different bioassays. Water Research.
- , 25-32.
- Helma C., S. Knasmuller , and R. Schulte Herman.
- Bie Belastung von Wassern mit
- genotoxischen Substanzen I. Methoden zur
- Prufung der Genotoxizita. Z. Umweltchem
- Okotox 6, 277-288.
- Ivanova, E., A. S. Teodora, and I. Velcheva. 2005.
- Cytogenetic testing of heavy metal and
- cyanide contaminated river waters in a mining
- region of Southwest Bulgaria. Journal of Cell
- and Molecular Biology. 4, 00-106.
- Knasmuller, S., E. Gottman, H. Steinkellner, A.
- Fomin, Pickl C, A. Paschke, R. God, and M.
- Kundi.1998. detection of genotoxic effects of
- heavy metal contamination soils with plant
- bioassays. Mutation Research., 2720; 000-000.
- Knasmuller, S., C. Helma, P. M. Eckel, E.
- Gottman, H. Steinkellner, F. Kassie, T. Haider,
- W.Parzefall, and R. Schulte-Hermann. 1998.
- Investigation on genotoxic effects of
- groundwater from the Mitterndorfer Senke and
- from the vicinity of Wiener Neustadt. 110, 23,
- -833.
- Kundi.1998. detection of genotoxic effects of heavy
- metal contamination soils with plant bioassays.
- Mutation Research., 2720;000-
- micronucleus (TradMCN) bioassay on
- clastogenicity of wastewater and in situ
- monitoring. Mutat. Res. 270, 45-51.
- Ma, T.H., 1982. Tradescantia cytogenetic tests
- (root-tip mitosis, pollen mitosis, pollen mother
- âââ cell meiosis). Mut. Res., 99, 293-302.
- Ma, T.H., Z. Xu, C. Xu, H. McConnell, E. V.
- Rabago, G.A. Arreola and H. Zang. 1995. The
- improved Allium/Vicia root tip micronucleus
- assay for clasto genicity of environmental
- pollutants. Mutation Research - Environmental
- Mutagenesis and related Subjects. 334 (2),
- -195.
- Majer, B., D. Tscherko, A. Paschke, R. Wennrich,
- M. Kundi, E. Kandellr and S. Knasmuller.
- Effects of heavy metal contamination of
- soils on micronucleus induction in Tradescantia
- and microbial enzyme activities: a
- comparative investigation. Mutation Research.
- , 111-124.
- Matsumoto, S.T., M.S. Mantovani, M. Irene, A.
- Malaguttii, A.L. Dias, I. C. Fonseca and M.A.
- Marin-Morales. 2006. Genotoxicity and
- mutagenicity of water contaminated with
- tannery effluents, as evaluated by
- micronucleus test and comet assay using fish
- Oreochromis niloticus and chromosome
- aberrations in onion root-tips. Genetics and
- Molecular Biology. 29 (1), 148-158
- Misk, M., T.H., Ma, A. Nersesyan, S. Monarca,
- J.K. Kim and S. Knasmuller. 2011.
- Micronucleus assays with the Tradescantia
- pollen tetrads: and update. Mutagenesis., 26
- (1), 215-221.
- Odeigah, P.G.C., O. Nurudeen, and O.O. Amund .
- Genotoxicity of oil field wastewater in
- Nigeria. Hereditas. 126, 161-167.
- Rizaj, M., Beqiri E, McBow, Oâââ¢Brein E.Z,
- Kongoli F, 2008. The mineral base and
- productive capacities of metals and nonmetals
- of Kosovo. JOM., vol.60 No.8:18-22.
- Ruiz, E. F., V. M. E. Rabago, S. U. Lecona, A. B.
- Perez and T. H. Ma. 1992. Tradescantia âââ
- micronucleus (Trad-MCN) bioassay on clasto
- genicity of wastewater and in situ monitoring.
- Mutat. Res. 270, 45-51.
- Samka-Kinci, V., P. Stegnar, M. Lovka and M. J.
- Toman. 1996. The evaluation of waste,
- Surface and ground water quality using the
- Alium test procedure. Mutation Research/
- Genetic Toxicology. 368 (3-4), 171-179.
- Samuel , O.B., F.I. Osuala and P. G. C. Odeigah.
- Cytogenotoxicity evaluation of two
- industrial effluents using Allium cepa assay.
- African Journal of Environmental Science and
- Technology. 41 (1), 21-27.
- ZSandhu, S.S., T.H. MA, Y. Peng and H. Zhou.
- Clastogenicity of seven chemicals
- commonly found at industrial waste sites.
- Mutat. Res. 224, 437-445.
- Shokal R.R., and F.J. Rholf. 1981. Biometry, 2nd
- Edn., Freeman. New York, 1981, pp. 427-428.
- Steinkenller, H., F. Kassie, and S.
- Knasmuller.1999. Tradescantia âââ micronucleus
- assay for the assessment of the clastogenicity
- of Austrian water. Mutation Research. 426,
- -116.
- Tolbert, P. E., C. M. Shy, and J.W. Allen. 1992.
- Micronuclei and other ano- malies in buccal
- smears: methods Mutation Research, 271, 69-
- Zahn, R. K., H. C. Schroder, and H. G.
- Miltenburger. 1992