بررسی استریولوژیک و بیوشیمیایی اثرات تیامین و اکسید روی بر روی سکوم پس از پانکراتیت تجربی ناشی از ال- آرژنین در موش صحرایی
محورهای موضوعی : تشریح
رحمت الله فتاحیان دهکردی
1
,
سورن نورائی
2
,
البرز یدالهی
3
,
پارسا رحمانی
4
1 - گروه علوم تشریحی، گروه علوم پایه، دانشکده دامپزشکی، دانشگاه شهرکرد، شهرکرد، ایران
2 - موسسه تحقیقات واکسن و سرم سازی رازی، سازمان تحقیقات، آموزش و ترویج کشاورزی، شیراز، ایران
3 - گروه علوم تشریحی، گروه علوم پایه، دانشکده دامپزشکی، دانشگاه شهرکرد، شهرکرد، ایران
4 - گروه علوم تشریحی، گروه علوم پایه، دانشکده دامپزشکی، دانشگاه شهرکرد، شهرکرد، ایران
کلید واژه: التهاب پانکراس, موش صحرایی, هیستومورفومتری, ارزیابی بیوشیمیایی, نانوذرات اکسید روی,
چکیده مقاله :
پانکراتیت حاد (AP) یک بیماری شایع و بحرانی با میزان مرگ و میر بالا به دلیل نکروز پانکراس و برخی از اندام ها است. هدف ما بررسی اثر تیامین و نانو ذره اکسید روی در درمان پانکراتیت حاد بود.روش کار :50 موش صحرایی با میانگین وزن 28 تا 32 گرم به طور تصادفی به پنج گروه تقسیم شدند: گروه 1: کنترل، 2: گروه ال-آرژنین (300 میلی گرم/100 گرم)، 3: گروه تیامین (70 میلی گرم بر کیلوگرم)، 4: گروه نانوذرات اکسید روی (1 میلی گرم بر کیلوگرم)، 5: گروه درمان ترکیبی: ال-آرژنین (300 میلی گرم/100 گرم) + تیامین (70 میلی گرم بر کیلوگرم) + نانوذرات اکسید روی (1 میلی گرم بر کیلوگرم). پس از بررسی استریولوژی برش های بافتی و اندازه گیری بیومارکرها، داده ها در سطح معنی داری 05/0p≤ تجزیه و تحلیل شدند. بر اساس نتایج، در گروه تیمار ال-آرژنین ضخامت لایه‌های مخاطی، عضلانی و اضافی به همراه ضخامت لایه‌های کل کاهش معنی‌داری را نشان داد (05/0p≤). همچنین در مقایسه با گروه کنترل ارزیابی سطح و تراکم لایه های مخاط و عضلانی، کاهش را همچون سطح و حجم کلی لایه ها نشان داد در حالی که افزودن تیامین و نانوذرات اکسید روی به ال-آرژنین این پارامترها را افزایش داده است. ارزیابی کلسترول افزایش سطح معنی داری را بین گروه کنترل با گروه 5 و گروه 4 ذکر کرد. تجزیه و تحلیل فاکتورهای ALT و AST حاکی از افزایش معنی دار سطح AST در گروه ال-آرژنین در مقایسه با گروه کنترل و کاهش معنی دار در سطح ALT در گروه تیامین و نانوذرات اکسید روی در مقایسه با گروه ال-آرژنین بود. بر اساس نتایج تحقیق حاضر، نانوذرات تیامین و اکسید روی اثرات افزایشی بر ضخامت، سطح و تراکم حجمی و میزان فاکتور بیوشیمیایی ALT نشان دادند.
Acute pancreatitis (AP) is a critical disease with a high mortality rate due to the necrosis of the pancreas and other organs. We aimed to study the effects of thiamine and ZnO nanoparticles (NPs) in treating AP. Fifty male rats, with an average weight of 28 to 32 grams, were randomly divided into five groups: 1: Control group, 2: L-arginine group (300 mg/100g), 3: Thiamine group (70 mg/kg), 4: ZnO NPs group (1 mg/kg), 5: Combination treatment group: L-arginine (300 mg/100g) + thiamine (70 mg/kg) + ZnO NPs (1 mg/kg). After stereological examination of tissue sections and measurement of biomarkers, the data were analyzed at a significance level of p≤ 0.05. According to the results, in the L-arginine treatment group, the thickness of the mucosal, muscular, and adventitial layers decreased in comparison to the total thickness (p≤ 0.05). Additionally, values of the surface area and density of the mucosal and muscular layers decreased, as well as the total values of these, compared to the control group. The mentioned parameters increased in the combination treatment group. Evaluation of cholesterol showed a significant increase between the control group and groups 4 and 5. Analysis of ALT and AST factors indicated a significant increase in the level of AST in L-arginine group compared to the control group, and a significant decrease in the level of ALT in comparison between the thiamine and ZnO NPs groups and the L-arginine group. Based on the results of the present research, thiamine and ZnO NPs manifested enhancing effects on the values of thickness, surface area, volume density, and level of ALT.
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