Molecular identification gene of the Helicobacter pylori neutrophil activating protein effective in controlling allergic asthma by PCR method
Alireza Khaleghi Khorrami
1
(Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Zanjan Branch, Zanjan, Iran)
Sanaz Mehmazi
2
(Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Zanjan Branch, Zanjan, Iran)
Rasul Shukri
3
(Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Zanjan Branch, Zanjan, Iran)
Seyyed Shamsadin Athari
4
(Department of Immunology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran)
کلید واژه: Helicobacter pylori, allergic asthma, neutrophil activating protein,
چکیده مقاله :
Objective: Helicobacter pylori neutrophil activating protein (HP-NAP) is one of the important proteins of Helicobacter pylori (H.pylori). HP-NAP has immunomodulatory properties and directs allergic responses from Th2 to Th1. The aim of this study is the molecular identification of HP-NAP gene native to Iran. Method: The native strain of H.pylori was purchased from the Pasteur Institute, then specific urease test was performed, as well as gram staining and observation under the microscope, and in order to identify HP-NAP in native bacteria, this was done by PCR method. Finally, the PCR product should be electrophoresed to detect the HP-NAP gene. Results: After bacterial staining and microscopic observation, bent gram-negative bacteria were observed. Also, the result of the urease test was seen with a change in urea reagent (phenol red), which indicated H.pylori. The result of the PCR test and perform electrophoresis, the 260 bp band related it was the HP-NAP gene was seen in comparison with ladder 100. Conclusion: According to the study conducted and the confirmation of the presence of this protein in the structure of native bacteria, as well as previous studies in different regions of the world and observing the effects of this protein as an immunomodulatory of the immune system, we can hope that in the future this protein will be used as a It has the potential to control and treat allergic diseases, especially allergic Asthma.
چکیده انگلیسی :
