DNA interaction and antimicrobial studies of novel copper (II) complex having ternary Schiff base

محورهای موضوعی : Journal of the Iranian Chemical Research

Natarajan Raman ¹ , Thanasekaran Baskaran ² , Abraham Selvan ³ , Ramaraj Jeyamurugan ⁴

1 - Research Department of Chemistry, VHNSN College, Virudhunagar 626 001, India
2 - Research Department of Chemistry, VHNSN College, Virudhunagar 626 001, India
3 - Research Department of Chemistry, VHNSN College, Virudhunagar 626 001, India
4 - Research Department of Chemistry, VHNSN College, Virudhunagar 626 001, India

تاریخ دریافت : 1387/03/17 تاریخ پذیرش : 1387/10/05 تاریخ انتشار : 1386/10/30

کلید واژه: Ternary Schiff base, DNA binding constant, Intercalative binding mode, Redox properties, CT DNA cleavage, Antimicrobial assay,

چکیده مقاله :

A novel ternary Schiff base ligand (HL) of ONO type and its copper (II) complex weresynthesised using 2-aminophenol and o-acetoacetotoluidide. They have been characterised by theusual analytical and spectral methods. The interaction of the complex with calf-thymus (CT)DNA has been investigated by UV-Vis, viscosity measurement, cyclic voltammetry anddifferential pulse voltammetry studies. The results show that the complex binds to CT DNA byintercalating way. The CT DNA cleavage study was monitored by gel electrophoresis method.From this study, it was found that the complex cleaves CT DNA in presence of the oxidant,hydrogen peroxide. The in vitro antimicrobial activities of the synthesized compounds have beentested against the bacteria Salmonella typhi, Staphylococcus aureus, Bacillus subtilis,Escherichia coli, Pseudomonas putita and Klebsiella pneumoniae and fungi Aspergillus niger,Aspergillus flavus, Rhizopus stolonifer, Candida albicans and Rhizoctonia bataicola by welldiffusion method. The Minimum Inhibitory Concentration (MIC) value against the growth of theabove microorganisms is much larger for copper complex than the ligand.

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