Promotion of Seed Germination and Seedling Growth in Zygophyllum atriplicoides using Chemical, Mechanical, and Biological Priming Treatments
محورهای موضوعی : Eco-physiology
Seyed Hassan Kaboli
1
(Assistant Professor, Department of Arid Land Management, Faculty of Desert Studies, Semnan University, Iran,)
Neda Ebrahimi Mohamad Abadi
2
(Desert studies Factuly, Semnan University)
Farhad Rejali
3
(Associate Professor, Soil Research Institute, Agricultural Extension and Education Karaj, Iran)
Ali Asghar Zolfaghari
4
(Associate Professor, Department of Arid Land Management, Faculty of Desert Studies, Semnan University, Iran)
کلید واژه: germination, Salicylic acid, Gibberellic acid, Ultrasonic, Bio priming,
چکیده مقاله :
Native palatable species are the most important plants for the restoration of arid areas. Zygophyllum atriplicoides is one of the useful species in restoring arid regions, but poor germination and seedling growth are the main problems in the establishment of this species. The objective of this study was to investigate the effect of biological, chemical, and mechanical priming treatments on seedling germination. The experiments were carried out in a completely randomized design with three replications in a laboratory (germinator) and tray culture mid-summer of 2018. Experimental treatments consisted of two chemical treatments including salicylic acid and gibberellic acid at three levels, five biological treatments consist of Azotobacter chroococcum, Azospirillum lipoferum, Bacillus megaterium, Flavobacterium sp. and Pseudomonas fluorescens and mechanical treatments including 24 kHz ultrasonic wave during 5 min. Seed germination and seedling growth traits were measured in different treatments. Analysis of variance showed significant differences between treatments for all traits in both experiments (p<0.01 and p<0.01). The result of the laboratory experiment showed the higher mean values of germination percent (38.33), germination rate (14.69), germination percent day (2.73) and germination index (0.54) in Bacillus treatments than other treatments. Also, Azospirillum increased seed vigor index (56.0), root length (1.33cm), seedling length (2.26cm), fresh weight Root (8.40g), root dry weight (1.20g) and shoot dry weight (1.90g) as compared to control. The maximum shoot length and root to shoot length ratio were obtained by salicylic acid and gibberellic acid 3000 ppm, respectively. Bacillus caused the highest root length (4.73cm), root to shoot ratio (2.20), root (6.50g) and shoot fresh weight (2.63g) and leaf area (11.37). Maximum seedling length and leaf fresh and dry weight were obtained by Pseudomonas fluorescens. According to the results of this study, the use of bacteria, especially Azospirillum, Bacillus and Pseudomonas were effective in better germination of seed.
Abbasi Khalki, M., Alaei, N., Parchami, N., Shahabi, A., 2015. Investigation of the effects of different treatments on breaking dormancy and stimulating seed germination of Zygophyllum atriplicoides. Second National Conference on Conservation of Natural Resources and Environment. 3-4 March, University of Mohaghegh Ardabili, Ardebil, Iran. (In Persian).
Abdel-Haleem, A., El-Shaieny, H., 2015. Seed germination percentage and early seedling establishment of five (Vigna unguiculata l. Walp) genotypes under salt stress. European Journal of Pelafia Research Library, 5(2): 22-32.
Abdul-Baki, A.A., Anderson, J.D., 1973.Vigor determination in soybean seed by multiple criteria. Journal of Crop Science, 13(6): 630-633.
Abdulsalam, A., Abula, d., Kai, ZH., Tuerhong, M., Abdulrashid, K., Ling, L., 2019. Fruit set and seed germination traits of Zygophyllum kaschgaricum. Chinese Journal of Plant Ecology, 43(5): 437-446.
Ahmadi, H. 2012. Applied Geomorphology Volume 2 (Desert Wind Erosion). University of Tehran Publications, Iran. 706 pp. (In Persian).
Akhgar, M.R., Rajaei, P. and Poshteshirani, F., 2015. Composition of the Essential Oil of Zygophyllum atriplicoides from Iran. Chemistry of Natural Compounds, 51(3): 577-578.
Al Khateeb, W.M., Muhaidat, R.M., Odat, N., Sawaie, A., Lahham, J.N., and Al-Oqlah, A., 2010. Interactive effects of salinity, light and temperature on seed germination of Zygophyllum simplex L. Zygophyllaceae). International Journal of Integrative Biology, 10(1): 9-13.
Alvani, F., Dianati Tilaki, G.A., Sadati, E., 2018. The effects of seed priming with acid ascorbic on seed germination and morphological traits of Taverniera cuneifolia under drought stress. Journal of Rangeland Science, 8(3): 264-271.
Amoo, S.O., Ojo, A.U., Van Staden, J., 2008. Allelopathic potential of Tetrapleura tetraptera leaf extracts on early seedling growth of five agricultural crops. South African Journal of Botany, 74(1): 149-152.
Azarnivand, H., 2003. Studying botanical and ecological characteristics of Artemisia sieberi and Artemisia aucheri in the southern slopes of Alborz Mountain. PhD Thesis in the Rangeland Science, Faculty of Natural Resources, Tehran University, Tehran, Iran. 180 pp. (In Persian).
Balaguera-López, H.E., Cárdenas-Hernández, J.F., Álvarez-Herrera, J.G., 2008. Effect of gibberellic acid (GA3) on seed germination and growth of tomato (Solanum lycopersicum L.). In International Symposium on Tomato in the Tropics, 821: 141-148.
Biabani, A., Zarei, M., Sancholi, S., Romani, A., 2017. The effect of temperature and duration of the placement of seeds at different temperatures on seed germination of barley. Applied Research of Plant Ecophysiology, 4(1): 173-186. (In Persian).
Commander, L.E., Merritt, D.J., Rokich, D.P., Dixon, K.W. 2009. Seed biology of Australian arid zone species: Germination of 18 species used for rehabilitation. Journal of Arid Environments, 73(6): 617-625.
Drogue, B., Doré, H., Borland, S., Wisniewski-Dyé, F. & Prigent-Combaret, C. 2012. Which specificity in cooperation between phytostimulating rhizobacteria and plants? Research in Microbiology, 163(8): 500-510.
Gray, E. J. & Smith, D. L. 2005. Intracellular and extracellular PGPR: commonalities and distinctions in the plant–bacterium signaling processes. Soil Biology and Biochemistry, 37(3): 395-412.
Hampton, J.G., Tekrony, D.M., Chairperson, D. 1995. Handbook of vigor test methods. The International Seed Testing Association, Zurich. 117 pp.
Janda, T., Szalai, G., Tari, I., Paldi, E., 1999. Hydroponic treatment with salicylic acid decreases the effects of chilling injury in maize (Zea mays L.) plants. Journal of Planta, 208(2): 175-180.
Khatibzadeh, R., Azizi, M., Aroie, H., Farsi, M., 2013. The effect of surface disinfection and stratigraphic treatments on seed germination of Roman ginger (Levisticum officinale Koch.) In vitro conditions. Journal of Horticultural Science, 27(2): 130-138. (In Persian).
Kloepper, J.W., F.M. Scher., M. Laliberte., Tipping, B., 1986. Emergence promoting rhizobacteria: description and implications for agriculture. In: Swinburne, T.R. (Ed.), Iron, Siderophore and Plant Diseases. Pelnum Publishing Company, New York. 155–164.
Mishra, B. K., Lal, G., Sharma, Y. K., Kant, K., Saxena, S. N., Dubey, P.N., 2019. Effect of microbial inoculants on cumin (Cuminum cyminum Linn.) growth and yield. International J. Seed Spices, 9(1): 53-56.
Moghimi, c., 2006. Introduction of Some Important Rangeland Species. Aaron Publications, Tehran, Iran. 669 pp. (In Persian).
Moghaddam, S. S., Rahimi, A., Noorhosseini, S. A., Heydarzadeh, S., Mirzapour, M. 2018. Effect of Seed Priming With Salicylic Acid on Germinability and Seedling Vigor Fenugreek (Trigonella Foenum-Graecum). Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi, 28(2): 192-199.
Mozaffarian, V., 2006, A Dictionary of Iranian Plant Names, Farhang Moaser, Tehran, Iran. 592 pp. (In Persian).
Pereg, L., de-Bashan, L. E., Bashan, Y., 2016. Assessment of affinity and specificity of Azospirillum for plants. Plant and Soil, 399(1): 389–414.
Rafatpour, Sh., Shahriari, AR. 2013. Effects of priming and sodium chloride on germination and seedling growth of Zygophyllum (Zygophyllum atriplicoides). Journal of Desert Ecosystem Engineering. 1(2): 24-15. (In Persian).
Rozier, C., Gerin, F., Czarnes, S., Legendre, L., 2019. Biopriming of maize germination by the plant growth-promoting rhizobacterium Azospirillum lipoferum CRT1. Journal of plant physiology, 237: 111-119.
Sabeti M., Ghehsareh Ardestani E., tahmasebi P., Nikookhah F., 2019a. Effects of Seed Biopriming on Some Characteristics of the Germination and Growth of Astragalus ovinus Boiss under Drought Stress, Desert Management, 7(13): 49-64.
Sabeti, M., Tahmasebi, P., Ghehsareh Ardestani, E., Nikookhah, F., 2019b. Effect of Plant Growth Promoting Rhizobacteria (PGPR) on the Seed Germination, Seedling Growth and Photosynthetic Pigments of Astragalus caragana under Drought Stress. Journal of Rangeland Science, 9(4): 364-377.
Sanginabadi, H., Khorasaninejad, S. Hemmati., Kh., Ghasemnejad, A. 2016. A study on propagation methods of Lavandula stricta Del. Iranian Journal of Medicinal and Aromatic Plants, 32(3): 417-427. (In Persian).
Schelin, M., Tigabu, M., Eriksson, I., Swadago, L., Oden, P.C., 2003. Effect of scarification, gibberellic acid and dry heat treatments on the germination of Balanties Egyptian seed from the Sudanian savanna in Burkina Faso. Journal of Seed Science Technology, 31: 605–617.
Seyed Sharifi, R., Khawazi, K., 2011. Effect of plant growth-promoting bacteria on germination and seedling growth components of maize (Zea mays L.). Agricultural Ecology, 3(4): 513-506. (In Persian).
Sharififar, A., Nazari, M., Asghari, H. R., 2015. Effect of ultrasonic waves on seed germination of Atriplex lentiformis, Cuminum cyminum, and Zygophyllum atriplicoides. Journal of Applied Research on Medicinal and Aromatic Plants, 2(3): 102-104.
Shawky, E., Gabr, N., El-gindi, M., Mekky, R., 2019. A Comprehensive Review on Genus Zygophyllum. Journal of Advanced Pharmacy Research, 3(1): 1-16.
Sheikh Ghahfrokhi, F., 2014. Investigation of effects of seed inoculation with growth-promoting bacteria on the improvement of germination, growth and yield indices of Calendula (Calendula officinalis L.). Master of Science Degree in Agricultural Science and Technology. School of Agriculture, Shahrekord University, Iran. (In Persian).
Shimon, M., Tirosh, T., Glick, B.R., 2004. Plant growth-promoting bacteria confer resistance in tomato plant to salt stress. Plant Physiology and Biochemistry, 42(6): 565-572.
Shirinzadeh, A., Soleimanzadeh, H., Shirinzadeh, Z., 2013. Effect of seed priming with plant growth-promoting rhizobacteria (PGPR) on agronomic traits and yield of barley cultivars. World Applied Sciences Journal, 21(5): 727-731.
Soltanipour, M.A, Asadpour, R., Bagheri, R., 2011. Study of pretreatments on seed germination of Zygophyllum atriplicoides, Environmental erosion researches, 1(2): 69-82.
Tigrine-Kordjani, N., Meklati, B.Y., Chemat. F., 2011. Contribution of microwave accelerated distillation in the extraction of the essential oil of the Zygophyllum album L. Phytochemical Analysis, 22(1): 1-9.
Yanik, F., Ayturk, O., Cetinbas-Genc, A., Vardar, F., 2018. Salicylic acid-induced germination, biochemical and developmental alterations in the rye (Secale cereale L.). Acta Botanica Croatica, 77(1): 45-50.
