Effects of Drought Stress and Mycorrhiza on Viability and Vegetative Growth Characteristics of Ziziphora clinopodioides Lam.
محورهای موضوعی : Medicinal Plants
Reyhaneh Azimi
1
(Ph.D. Rangeland Sciences, Researcher of arid environments Research Center)
gholam Ali Heshmati
2
(Rangeland Department, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran)
Mohammad Kia Kianian
3
(Academic Member of Desert Studies Faculty, Semnan University, Iran)
کلید واژه: drought, Biological fertilizer, Colonization, Greenhouse,
چکیده مقاله :
In order to study the effects of drought stress and mycorrhiza fungi on establishment rate and some growth-morphological traits ofZiziphora clinopodoides, pot experiments were conducted as factorial design based on a completely randomized design at the research greenhouse of agricultural faculty, Ferdowsi University of Mashhad in 2014; pots were combined as four levels of drought stress including 100 (Control), 75, 50 and 25% of Field Capacity (FC) and three levels of mycorrhiza fungi including Glomus intraradices, Glomus versiforme and control. The results showed that drought stress of 25%FC decreased plants weight (0.75, 0.67 and 0.19g/pot), plant height (1.25, 1.18 and 0.7cm), establishment rate (67.50%, 57% and 14.25%) and plants root colonization by mycorrhiza fungi (50%, 35.5% and 0) by the application of G. intraradices, G. versiforme and control, respectively indicating significant effects of both Mycorrhiza species on drought resistance. Result indicated that the establishment rate was decreased significantly to 85%, 64% and 36% using G. intraradices, and G. versiforme and control at 50%FC, respectively. Similarly, the establishment rate was reduced in the inoculated plants with mycorrhiza treatments less than control (67.50%, 57% and 14.25% at 25%FC) so that symbiosis of Mycorrhiza fungi significantly improved Z. clinopodoides establishment rate under drought stress against the control. The effect of G. intraradices and G. versiforme on plants establishment rate (93% against 78.50% at 75%FC), plants height (5.93cm against 5.30cm at 75%FC) and dry weights (1.07g against 0.95g at 50%FC) was higher through drought stress. The Glomus intraradices reacted better to mild drought than G. versiforme and increased the establishment and morphological traits of plant. According to results, G. intraradices could be introduced as a biological fertilizer and a technique for retrofitting and increasing tolerance of Z. clinopodoides against drought stress.
Abdelmoneim, T.S., Moussa, T. AA, Almaghrabi, O.A., Alzahrani, S.H. and Abdelbagi, I., 2014. Increasing Plant Tolerance to Drought Stress by Inoculation with Arbuscular Mycorrhiza Fungi. Life Science Jour., 11(1): 10-17.
Abdul-Naser, A., 1998. Effects of inoculation Glomus intraradices on growth, nutrient uptake and metabolic activities of squash plants under drought stress condiction. Annuals of Agricultural Science Cairo, 1: 119-133.
Alkira, B.H., Simon, J.E., Palevitch, D. and Putievsky, E., 1993. Water management for Midwestern peppermint (Mentha piperita L.) growing in highly organic soils. Indiana, USA. Acta Horticultureae, 344: 544-556.
Almagrabi, O.A. and Abdelmoneim, T.S., 2012. Using of Arbuscular mycorrhiza fungi to reduce the deficiency effect of phosphorous fertilization on maize plants (Zea mays L.). Life Science Jour., 9 (4):1648-1654.
Amiri Dehabadi, A., Rezvani Moghaddam, P. and Ehyaee, H. R., 2012. The effect of drought on yield and some morphological traits of three medical plants dill (Anethum graveolens), coriander (Coriandrum sativum) and fennel (Foeniculum vulgare) under greenhouse conditions. Iran Agricultural Research, 10 (1): 116-124. (In Persian).
Auge, R.M., 2001. Water relation drought and vesicular arbuscular mycorrhiza symbiosis. Mycorrhiza, 11: 3-42.
Azimi., R., 2013. Investigating effects of mycorrhiza inoculation on the establishment and growth characteristics Bromus kopetdaghensis, Medicago sativa, Thymus vulgaris and Ziziphora clinopodioides in rangeland of Bahar Kish Quchan. Thesis in M.Sc., Ferdowsi Mashhad University. 167 p. (In Persian).
Azimi, R., Jankju,M. and Asghari, H., 2014a. Effect of mycorrhiza inoculation on seedlings establishment and morphological parameters of alfalfa (Medicago sativa L.) in rangeland ofBahar Kish Quchan. Agroecology, 5(4): 424- 432. (In Persian).
Azimi, R., Jankju, M. and Asghari, H., 2014b. Effects of mycorrhiza Symbiosis on Initial Establishment and Morphological Traits of Thyme (Thymus vulgaris) Under Natural Conditions. Iranian Jour. Field Crops Research, 11(4): 666-676. (In Persian).
Azimi, R., Jankju,M. and Asghari, H., 2015. Effect of mycorrhiza inoculation on seedlings establishment and morphological parameters of Bromus kopetdaghensis L. under field conditions. Jour. Range and Watershed Management (Iranian Jour. Natural Resources), 67(4): 559-570. (In Persian).
Bagheri, V., Shamshiri, M., Shirani, H. and Rusta, H., 2011. The effect of Mycorrhiza arbuscular and drought stress on growth, water relationship, the accumulation of proline and soluble sugar in seedlings of two basic variety of domestic pistachio (Pistacia vera L.). Iranian Jour. Horticultural Sciences, 42 (4): 365-377 (In Persian).
Bahrami Sirmandi, S., Azimi, M., Naghizade, M., Hasanzade, R., Arman, M. and Farahnak, A., 2011. Mycorhiza Symbiosis. Behta Research Press, Esfahan. (In Persian).
Bethlenfalvay, G.J., Brown, M.S., Ames, R.N. and Thomas, R.S., 1988. Effects of drought on host and endophyte development in mycorrhiza soybeans in relation to water use and phosphate uptake. Physiol Plant, 72:565–571.
Busquets, M., Calvet, C., Camprubí, A. and Estaún, V., 2010. Differential effects of two species of arbuscular mycorrhiza on the growth and water relations of Spartium junceum and Anthyllis cytisoides. Symbiosis, 52:95–101.
Caravaca, F., Berea, J., Palenzuela, J., Figueroa, D., Alguacil, M. and Roldan, A., 2003. Establishment of shrub species in a degraded semiarid site after inoculation with native or Allochthonous arbuscular mycorrhiza fungi. Jour. Applied Soil Ecology, 22(2): 103-111.
Demir, S., 2004. Influence of arbuscular mycorrhiza on some physiological growth parameters of pepper. Turk Jour. Biology, 28: 85-90.
Giovannetti, M. and Mosse, B., 1980. An evaluation of techniques for measuring vesicular-arbuscular mycorrhiza infection in roots. Journal of New Phytologist, 84: 489-500.
Gonzalez-Chavez, C., D'Haen, J., Vangronsveld, J. and Dodd, J. C., 2002. Copper adsorption and accumulation by the external mycelium of three arbuscular mycorrhiza fungi from polluted soils. Jour. Plant and Soil, 240(2): 287–297.
Graham, J.H. and Syvertson J.P., 1984. Influence of vesicular arbuscular mycorrhiza on the hydraulic conductivity of roots of two citrus rootstocks. New Phytol, 97:277–284.
Hu, Y. and Schmidhalter, U., 2005. Drought and salinity: A comparison of their effects on mineral nutrition of plants. Jour. Plant Nutrition, 168: 541-549.
Jacobsen, I., Abbott, L. K. and Robson, A., 1992. External hyphae of Vesicular arbuscular Mycorrhiza fungi associated with Trifolium subterraneum L. I. Spread of hyphae and phosphorus inflow into roots. Jour. New Phytologist, 120: 371-380.
Ladjal, M., Huc, R. and Ducrey, M., 2005. Drought effects on hydraulic conductivity and xylem vulnerability to embolism in diverse species and provenances of Mediterranean cedars. Jour. Tree Physiology, 25: 1109 –1117.
Mamadalieva, Z. N., Akramov, D.Kh., Ovidi, E., Tiezzi, A., Nahar, L., Azimova, Sh.S. and Sarker, D. S., 2017. Aromatic Medicinal Plants of the Lamiaceae Family from Uzbekistan: thnopharmacology, Composition, and Biological Activities, Essential Oils. Medicines, 4 (8): 1-12.
Misra, A.O. and Sriacastiva, N.K., 2002. Influence of water stress on Japanese mint. Jour. Herbs, Species and Medicinal Plants, 7: 51-58.
Mota, J.F., Medina-Cazorla, J.M., Navarro, F.B., Pérez-García, F.J., Pérez-Latorre, A., Sánchez-Gómez, P., Torres, J.A., Benavente, A., Blanca, G., Gil, C., Lorite, J., and Merlo, M.E., 2008. Dolomite flora of the baetic ranges glades (South Spain). Flora, 203: 359–375.
Phillips, J. M. and Hayman, D. S., 1970. Improved procedure for clearing roots and staining parasites and vesicular arbuscular mycorrhiza fungi for rapid assessment of infection. Jour. Transactions of the British Mycological Society, 55: 158–161.
Porras-Soriano, A., Soriano-Martín, M.L., Porras-Piedra, A. and Azcón, R., 2009. Arbuscular mycorrhiza fungi increased growth, nutrient uptake and tolerance to salinity in olive trees under nursery conditions. Jour. Plant Physiology, 166: 1350-1359.
Rahmatzade, S. and Khara, J., 2008. The effect of UV-C ray on growth and some morphological and physiological traits of symbiotic wheat seedlings with three Mycorhiza fungi species. Iranian Jour. Field Crops Research, 21 (1): 52- 63. (In Persian).
Smith, F.A., Grace, E. J. and Smith, S. E., 2009. More than a carbon economy: nutrient trade and ecological sustainability in facultative arbuscular mycorrhiza symbioses. Jour. New Phytologist, 182: 347–358.
Song, H., 2005. Effects of VAM on host plant in the condition of drought stress and its Mechanisms. Electronic Jour. Biology, 1(3): 44-48.
Van der Heijden, M.G.A. and Sanders, I., 2002. Mycorrhiza ecology. Jour. Mycorrhiza Ecology. Springer, Berlin, Heidelberg. 469 pp.
Van de Staaij, J.W.M., Rozema, J., Van Beem, A. and Aerts, R., 2001. Increased solar UV-B radiation may reduce infection by arbuscular mycorrhiza fungi (AMF) in dune grassland plants: evidence from five years of field exposure. Jour. Plant Ecology, 154: 171-177.
Vosatka, M. and Dodd, J.C., 1998. The role of different arbuscular mycorrhiza fungi in the growth of Calamagrostis villosa and Deschampsia flexuosa in experiments with simulated acid rain. Jour. Plant and Soil, 200: 251-263.
Whitmore, A.P. and Whalley, W.R., 2009. Physical effects of soil drying on roots and crop growth. Jour. Experimental Botany, 60(10): 2845-2857.
Wu, Q. S. and Xia, R.X., 2004. Effects of mrbuscular mycorrhiza fungi on plant growth and osmotic adjustment matter content of trifoliate orange seedlings under water stress. Jour. Plant Physiology and Molecular Biology, 30: 583-588.
Wu, Q.S. and Xia, R.X., 2006. Arbuscular mycorrhiza fungi influence growth, osmotic adjustment and photosynthesis of citrus under well-watered and water stress conditions. Jour. Plant Physiology, 163(4): 417-425.
Yordanov, I., Velikova, V. and Tsonev, T., 2003. Plant responses to drought and stress tolerance. Jour, Plant Physiology, 187-206.
