Germination Enhancement and Primary Establishment of Three Medicinal Plants

محورهای موضوعی : Seed Science and Technology

Ghasem Esmaeili ¹ , Askar Ghani ² , Seyyed Morteza Jabbarifar ³ , Nastaran Hemmati ⁴

1 - Department of Horticultural Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
2 - Department of Horticultural Science, Faculty of Agriculture, Jahrom University, PO BOX 74135-111, Jahrom, Iran
3 - Department of Horticultural Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
4 - Department of of Horticultural Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.

تاریخ دریافت : 1399/03/28 تاریخ پذیرش : 1399/07/16 تاریخ انتشار : 1400/01/12

کلید واژه: Ferulago angulata, chilling, Seed dormancy, Dorema aucheri, Echinophora cinerea,

چکیده مقاله :

Lack of cultivation of some Apiaceae family plants caused damage and uncontrolled exploitation of pastures and severe fluctuations in these plant markets. Therefore, efforts are essential to study the domestication of these species. For germination enhancement of three species including Dorema aucheri Boiss., Echinophora cinerea (Boiss.) Hedge & Lamondand Ferulag oangulata (Schlescht.) Boiss., six experiments in laboratory and greenhouse conditions were designed and performed during 2017-2018. At first, a completely randomized design factorial experiment with two factors (the first factor including 13 treatments consisted of stratification, soaking in GA3 or KNO3 and the second factor including stratification temperature consisted of 5, 10 and 15°C) was executed. In the second experiment, seedlings establishment was investigated in the different substrates. The results indicated that different treatments such as chilling, GA3, KNO3, and their integration had a low effect on Dorema aucheri seed germination. The effect of stratification at 4°C for 45 days on Echinophora cinerea (97.8%) and at 15°C for 45 days on Ferulago angulata (97.8%) was so clear on germination factors. The use of different growth media showed that studied species require relatively light medium and moisture during and after germination. Accordingly, mixed soil and cocopeat with 1:1 was the best substrate. It should be noted that the suitable medium for the primary growth of seeds was very similar to their natural habitats.

منابع و مأخذ:

Abedi, E., 2020. Effect of nanopriming for enhancing germination and seedling growth of Dorema aucheri L. 31st European Congress on Nanotechnology and Materials Engineering, Paris.

Amirghofran, Z., Bahmani, M., Azadmehr, A., Javidnia, K., 2006. Anticancer effects of various Iranian native medicinal plants on human tumor cell lines. Neoplasma, 53 (5), 428-433.

Asgari, M., Nasiri, M., Ashraf, J.A., Flah, H.L., 2015. Investigation of chilling effects on characteristics of seed germination, vigor and seedling growth of Nepeta spp. species. J. Rangel. Sci., 5 (4), 313-324.

Atia, A., Debez, A., Barhoumi, Z., Smaoui, A., Abdelly, C., 2009. ABA, GA₃ and nitrate may control seed germination of Crithmum maritimum (Apiaceae) under saline conditions. C. R. Biol., 332 (8), 704-710.

Cerabolini, B., De Andreis, R., Ceriani, R.M., Pierce, S., Raimondi, B., 2004. Seed germination and conservation of endangered species from the Italian Alps: Physoplexis comosa and Primula glaucescens. Biol. Conser., 117 (3), 351-356.

Christensen, L.P., Brandt, K., 2006. Bioactive polyacetylenes in food plants of the Apiaceae family: Occurrence, bioactivity and analysis. J. Pharmaceut. Biomed. Anal., 41 (3), 683-693.

Ebrahimian, V., Bagheri, R., Mohseni, M., Poormirzaei, A., 2013. Ecological and phenological study on Ferulago angulata in the Hezar Mountains and Bondar Henza, Kerman, Iran. J. Rangel. Sci., 3 (4), 277-285.

Fang, S., Wang, J., Wei, Z., Zhu, Z., 2006. Methods to break seed dormancy in Cyclocarya paliurus (Batal) Iljinskaja. Sci. Hortic., 110 (3), 305-309.

Ghadiri, H., Bagherani, T., 2000. Effect of scarification and temperature on germination of Licorice (Glycyrrhiza glabra L.) seeds. J. Agr. Sci. Technol. (JAST), 2 (4), 5257-262.

Ghani, A., Saharkhiz, M.J., Hassanzadeh, M., Msaada, K., 2009. Changes in the essential oil content and chemical compositions of Echinophora platyloba DC. during three different growth and developmental stages. J. Essent. Oil-Bear. Plants, 12 (2), 162-171.

Ghasemi-Arian, A., Izadi, J., afkham-Alshoara, S., Ejlali, R., 2009. Germination improvement in seeds of gum ammoniac (Dorema Ammoniacum). Iraian J. Rangel. Des. Res., 15: 455-463. (English abstract)

Gholami, B.A., Askarzade, M.A., 2007. Investigation of Dorema ammoniacum and Ferula assa-foetida cultivation in different times in order to domesticate them in Mashhad. National Conference on the Sustainable Development of Medicinal Plants, Mashhad, Iran. (In Persian)

Ginwal, H.S., Phartyal, S.S., Rawat, P.S., Srivastava, R.L., 2005. Seed source variation in morphology, germination and seedling growth of Jatropha curcas Linn. in Central India. Silvae Genet., 54 (1-6), 76-80.

Irvani, N., Solouki, M., Omidi, M., Saidi, A., Zare, A.R., 2012. Seed germination and dormancy breaking in Dorema ammoniacum. an endangered medicinal plant. Trakia J. Sci., 10 (1), 9-15.

Jahantab, E., Sepehri, A., Mirdelami, S.Z., Gasemi Arian, Y., Noori. S., 2012. Autecology investigation of Ferulago angulata (Schlecht) Boiss. in center Zagros (Kohgiluyeh Region). J. of Plant Environ. Physiol., 6 (4), 1-8. (In Persian)

Jalili, A., Jamzad, Z., 1999. Red data book of Iran : a preliminary survey of endemic, rare and endangered plant species of Iran. Research Institute of Forests and Rangelands, IR.

kazemi, E., Amrali S., Padyab, M., Shafiee A., Ghasemi-Arian, Y., 2011. Autecology of Dorema aucheri in rangeland ecosystems of Kohgiloye and Boyerahmad province. Iraian J. Rangel. Des. Res., 17 (4), 567-574. (English abstract)

Keshtkar, H.R., Azarnivand, H., Etemad, V., Moosavi, S.S., 2008. Seed dormancy-breaking and germination requirements of Ferula ovina and Ferula gummosa. Desert, 13 (1), 45-51. (English abstract)

Khanahmadi, M., Miraghaee, S.S., Karimi, I., 2012. Evaluation of the antioxidant and antimicrobial properties of Dorema aucheri plant. Iran. Red Crescent Med. J., 14 (10), 684-685.

Kumar Vashistha, R., Nautiyal, B.P., Nautiyal, M.C., 2009. Chemical stimulation of seed germination in Angelica archangelica Linn. (Apiaceae), a threatened high altitude aromatic herb. J. Am. Sci., 5 (5), 59-70.

Mellati, F., Parsa, M., Lalehgani, B., 2010. Evaluation of germination behaviour and sowing date in Dorema, Asafetida and Galbanum. Iranian Journal of Field Crops Research, 8 (3), 521–530. (English abstract)

Mostafavi, S.H., Fazilati, M., Mostafavi S.A., Vah-Habi, M.R., Mostafav, F., Omidvarinia, S., Zandi-Atashbar, N., Derakhshanian, H., Hajipoor, A.R., 2013. Hepatotoxicity of Dorema aucheri (Bilhar) in albino mice. Arch. Iran. Med., 16 (9), 530-532.

Mozaffarian, V., 2017. Identification of Medicinal and Aromatic Plants of Iran, 3rd ed., Farhange Moaser National Network of Research and Technology in Medicinal Plants and Iranian Medicine.

Mozaffarian, V., 2004. A Dictionary of Iranian Plant Names: Latin - English - Persian, Farhang Moaser.

Nadjafi, F., Bannayan, M., Tabrizi, L., Rastgoo, M., 2006. Seed germination and dormancy breaking techniques for Ferula gummosa and Teucrium polium. J. Arid Environ., 64 (3), 542-547.

Rahnama-Ghahfarokhi, A., Tavakkol-Afshar, R., 2007. Methods for dormancy breaking and germination of galbanum seeds (Ferula gummosa). Asian J. Plant Sci., 6 (4), 611-616.

Schelin, M., Tigabu, M., Eriksson, I., Sawadogo, L. and Oden, P.C., 2003. Effects of scarification, gibberellic acid and dry heat treatments on the germination of Balanites aegyptiaca seeds from the Sudanian savanna in Burkina Faso. Seed Sci. Technol., 31(3), 605-617.

Sharifi, H., Khajeh-Hosseini, M., Rashed-Mohassel, M.H., 2015. Study of seed dormancy in seven medicinal species from Apiaceae. Iranian J. Seed Res., 2 (1), 25-36. (English abstract)

Vandelook, F., Bolle, N., Van Assche, J.A., 2007. Seed dormancy and germination of the European Chaerophyllum temulum (Apiaceae), a member of a trans-Atlantic genus. Ann. Bot., 100 (2), 233-239.

Walck, J.L., Hidayati, S.N., 2004. Germination ecophysiology of the western North American species Osmorhiza depauperata (Apiaceae): implications of preadaptation and phylogenetic niche conservatism in seed dormancy evolution. Seed Sci. Res., 14 (4), 387-394.