Investigating the Effects of Pod Elimination on Salinity Tolerance in Annual Medic (Medicago scutellata L.)
محورهای موضوعی : Seed Science and TechnologyFarzaneh Fakhari 1 , Hossein Sadeghi 2
1 - College of Agriculture, Shiraz University, Shiraz, Iran.
2 - College of Agriculture, Shiraz University, Shiraz, Iran.
کلید واژه: Sodium chloride, ascorbate peroxidase, percentage of germination,
چکیده مقاله :
In most southern provinces of Iran, Soil Salinity is a growing problem particularly in the irrigated agricultural areas and has been found to reduce crop yields. In order to investigate the role of pod removal in seed germination, seedling growth and antioxidant enzymes activity in annual medics (Medicago scutella L.) under salinity stress conditions, a factorial experiment was conducted at College of Agriculture, Shiraz University in 2014 based on the completely randomized design with four replications. The factors included: 1) the presence or removal of seed pod, and 2) salinity stress at five levels involving controls 3, 6, 9 and 12 dS m-1. The results showed that salinity stress has caused the changes of germination, seedling growth and antioxidant enzymes activity. By increasing salinity stress levels, a significant reduction was observed in speed and percentage of germination as well as rootlet length, shoot length and seedling weight. However, the increased levels of salinity stress caused the increased antioxidant enzymes activity. Germination and seedling growth in seeds with no pod were more than seeds with pod, but about enzymes' activity, no significant difference was observed among seeds with and without pod, except for ascorbate peroxidase enzyme activity that was significantly more in seeds without pod as compared to the seeds with pod.
Abdul Qados, A.M.S., 2011. Effect of salt stress on plant growth and metabolism of Bean Plant (Vicia Faba L.). Jour. Saudi Soc. Agric. Sci., 10: 7-15.
Aghaei, K., Ehsanpour, A., Komatsu, S., 2009. Potato responds to salt stress by increased activity of antioxidant enzymes. Jour. Int. Plant Biol., 51:1095-1103.
Almansouri, M., Kinet, J.M., Lutts, S., 2001. Effect of salt and osmotic stress on germination in durum wheat (Triticum aestivum Desf.). Plant Soil, 231: 243-254.
Ashraf, M., Harris, P.J.C., 2004. Potential biochemical indicators of salinity tolerance in plants. Plant Sci., 166: 3-16.
Beauchamp, C. Fridorich, I., 1971. Superoxide dismutase improved assays and an assay applicable to acrylamide gels. Anal Biochem., 44: 276-287.
Bradford M.M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem., 72:248-254.
Broomand-Rezazadeh, G. Kucheki, A., 2005. Germination response of Ajowan, Fennel and Dill to osmotic potential of sodium chloride and polyethylene glycol 6000 in different temperature regimes. Iranian Jour. Agric Sci., 3: 207-217. (In Persian).
Chance, B., Maehly AC., 1955. Assay of Catalase and Peroxidase. Methods Enzymol., 2:764: 791.
Dehghani, I, Mostajeran, A., 2011. Effect of salinity on vegetative growth, antioxidant and defensive enzymes in ginger (Zingber officinale Roscoe.). Jour. Med Plants, 1: 1-11. (In Persian).
Esfandiari, E., Shekari, F. Shekari, F. Esfandiari. M., 2007. The effect of salt stress on antioxidant Enzymes activity and lipid peroxidation on the wheat seeding. Not Bot. Hort. Agro, 35: 48-56.
Gao, S., Ouyang, C., Wang, S., Xu, Y., Tang, L., Chen, F., 2008. Effect of salt stress on growth, antioxidant enzyme in phenylalanine Amonia-lyase activities in Jatropha curcas L. seedlings. Plant Soil Environ., 9: 374–381.
Ghamari-Zare, A., Rezvani, S., Forootan, M., 2009. Assessment of resistance to PEG-induced in drought in annual medic using aquaculture conditions. Iranian Jour. Rangelands Forests Plant Breed Genetic Res., 16: 182-197. (In Persian).
Ghanaatiyan, K., Sadeghi, H., 2015. Divergences in hormonal and enzymatic antioxidant responses of two Chicory ecotypes to salt stress. Planta Daninha, (Article in press).
Hosseini, H., RezvaniMoghadam, P., 2006. Effect of water and water salinity stress in seed germination on Isabgol (Plantago ovate). Iranian Jour. Agric Res, 4: 15-22. (In Persian).
Kaya, M.D., Okcu, G., Atak, M., Cikili, Y. Kolsarici, O., 2006. Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). European Jour. Agron., 24: 291-295.
Lynch, J., Lauchli, A., 1988. Salinity affects intracellular calcium in corn root protoplasts. Plant Physiol., 87: 351-356
Mass, E. V., Poss, J. A., 1989. Salt sensitivity of wheat at various growth stages. Irrig Sci, 10: 29-40.
Mehra, V., Tripathi, J. Powell, A. A., 2003. Aerated hydration treatment improves the response of Brassica juncea and Brassica campestris seeds to stress during germination. Seed Sci. Technol., 14: 57-70.
Miri, Y., Mirjalili S.A., 2013. Effects of salinity stress on seed germination and some physiological traits in primary stages of growth in purple coneflower (Echinacea Purpurea). Int. Jour. Agron. Plant Prod. 4:142-146.
Mirmohammadi Maibody, A.M., Qara Yazy, B., 2002. Salt stress and physiological aspects of plant breeding. Publishing Centre, University of Technology. 276pp. (In Persian).
Munir, N., Aftab. F., 2013. Changes in Activites of Antiozidant Enzymes in Response to NaCl Stress in Callus Cultures and Regenerated Plants of Sugarcane. Jour. Animal Plant Sci., 23:203-209.
Nakano, Y., Asada, K., 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant and Cell Physiology, 22: 867-880.
Pirasteh Anoshe, H., Sadeghi, H. Emam., Y. 2011. Chemical priming with urea and KNO3 enhances maize hybrids (Zea mays l.) seed viability under abiotic stress. Jour. Crop Sci. Biotechnol, 14: 289-295.
Sadeghi, H. and Khani, K., 2012. Effects of different drought and salinity stress levels on some morphological characteristics and proline content of annual burr medics (M. polymorpha L.). Jour. Dryland Res Iran, 1:1-13. (In Persian).
Sadeghi, H. and Khaef, N., 2011. Priming-induced metabolic changes in three annual medic species improve germination and early growth under drought and salt stress conditions. Genetics Plant Physiol, 1:186-198.
Sadeghi, H. and Nazemosadat, S., 2011. Effects of different levels of sodium chloride and Photosynthetic photon flux density on some physiological traits in two wheat cultivars. African Jour. Agric Res., 29:6326-6333.
Singh, BG., 1995. Effect of hydration-dehydration seed treatments on vigor and yield of sunflower. Indian Jour. Plant Physiol, 38: 66-68.
Tavakkol-Afshari, R. and Majnoun-Hossini, N., 2002. Responses of wheat and canola cultivars simulated drought conditions. Abstracts of international conference on environmentally sustainable agriculture for dry areas for 3rd millennium. China. September 16-19, p: 24-25.
Taylor, G.B, Rossiter, R.C and Palmer, M.J., 1984. Long-term patterns of seed softening and seedling establishment from single seed crop of subterranean clover. Aust Jour. Exp Agric, 24:200-212
Zhu, J.K., 2001. Plant salt tolerance. Trends Plant Sci., 6: 66–71.