یونش شیمیایی اسید آمینه والین در فشار اتمسفر با طیف سنج تحرک یونی مجهز به منبع یونش تخلیه کرونا
محورهای موضوعی : شیمی فیزیک
منیژه توضیحی
1
,
نسیم نجفلو
2
,
حامد بهرامی
3
1 - استادیار شیمی فیزیک، گروه شیمی، دانشکده علوم، دانشگاه زنجان، زنجان، ایران
2 - دانشجوی کارشناسی ارشد شیمی فیزیک، گروه شیمی، دانشکده علوم، دانشگاه زنجان، زنجان، ایران
3 - دانشیار شیمی فیزیک، گروه شیمی، دانشکده علوم، دانشگاه زنجان، زنجان، ایران
کلید واژه: اسید آمینه والین, طیف سنجی تحرک یونی, پروتونه شدن, تکه تکه شدن, یون های افزایشی.,
چکیده مقاله :
در این پژوهش، یونش شیمیایی اسید آمینه والین در فشار اتمسفری از راه برهم کنش آن با یون‌های هیدرونیم و آمونیم با طیف سنج تحرک یونی مجهز به منبع یونش تخلیه کرونا بررسی شد. نتیجه ها نشان داد که در حضور هیدرونیم به عنوان یون واکنشگر و آمونیم به عنوان دوپه کننده، یونش اسید آمینه والین از راه پروتونه شدن آن و تکه تکه شدن مولکول پروتونه شده و نیز تشکیل یون های افزایشی صورت می گیرد. دست کم شش گونه یونی به عنوان فراورده شناسایی شد. در طیف تحرک یونی والین دو نشانک به ترتیب به والین پروتونه شده و دوپار متقارن با مرز پروتون نسبت داده شد. تعیین دو نشانک دیگر از راه مقایسه طیف تحرک یونی والین و ایزوبوتیل آمین صورت گرفت. با معادله ارتباط جرم-تحرک بر پایه دو جرم استاندارد دو نشانک دیگر شناسایی شد. یکی از آن ها به یک خوشه یونی که از پیوند تکه یونی به مولکول خنثی والین به دست آمده، نسبت داده شد و دیگری به یک کربوکاتیون ناشی از تکه تکه شدن والین پروتونه از راه حذف آب مرتبط شد. با مطالعه تحول زمانی یون های فراورده و واکنشگر و نیز تغییر دمای محفظه دستگاه، منشاء پیشنهادی نشانک ها تایید شد. به این ترتیب بدون نیاز به جفت شدن دستگاه طیف سنج تحرک یونی به دستگاه طیف سنج جرمی، شناسایی فراورده های یونش انجام شد.
In this research, chemical ionization of valine amino acid in atmospheric pressure has been investigated through its interaction with hydronium and ammonium ions by using ion mobility spectrometer equipped with corona discharge ionization source. It was found that in the presence of hydronium as reactant ion and ammonium as dopant, the ionization of valine took place through its protonation, fragmentation of protonated molecule, and also formation of adduct ions. At least six ionic species were identified as product. In ion mobility spectrum of valine, two signals were assigned to the protonated valine and its symmetric proton-bound dimer, respectively. Identification of two other signals were conducted by comparison of the ion mobility spectrum of valine and isobutylamine. Using mass-mobility correlation equation based on two standard masses, two other signals were assigned. One of them was attributed to an ionic cluster obtained from the interaction of the ionic fragment with the neutral molecule of valine and the other one was related to a carbocation resulting from the fragmentation of protonated valine through the elimination of water. By the study of time, evolution of product and reactant ions signals and also the change of cell temperature, the proposed origin of the signals was confirmed. Therefore, the identification of ion products without need to couple the ion mobility spectrometer to the mass spectrometer was performed.
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