بررسی اثرات عصاره متانولی زعفران بر انسفالومیلیت خود ایمن تجربی در موشهای C57BL/6
شمیلا اسلامبولچی
1
(گروه زیست شناسی، دانشکده زیست شناسی، دانشگاه آزاد اسلامی- واحد تهران شمال، تهران، جمهوری اسلامی ایران)
محمد حسین صنعتی
2
(آزمایشگاه ژنتیک پزشکی، گروه ژنتیک پزشکی، پژوهشگاه مهندسی ژنتیک و زیست فناوری، تهران، ایران)
کمال الدین حق بین
3
(آزمایشگاه بیوشیمی، گروه زیست فرآورده های گیاهی، پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری، تهران، ایران)
کلید واژه: انسفالومیلیت خود ایمن تجربی, زعفران , میلین اولیگودندروسیت گلیکوپروتئین, ,
چکیده مقاله :
انسفالومیلیت خود ایمن تجربی (EAE) یک بیماری التهابی میلین زدای دستگاه عصبی مرکزی است و معمولا به عنوان یک مدل جانوری برای بیماری مالتیپل اسکلروزیس (MS) استفاده می شود. التهاب یک پدیده مهم در EAE و MS است که به از بین رفتن میلین و در پی آن وقوع ناتوانی حسی و حرکتی منجر می شود. چنین نشان داده شده است که عصاره های کلاله گیاه زعفران (.Crocus sativus L) در موشهای رت فعالیت ضد التهابی دارند. از سویی شواهد داروشناختی جدیدی درباره اثرات ضد توموری، محافظت ژنی، محافظت در برابر عوامل شیمیایی و همچنین توانایی رادیکال خواری برای عصاره های زعفران یا اجزای آن ارائه شده است. ما در این پژوهش مشاهده کردیم که استفاده از دگزامتازون یا عصاره های متانولی کلاله زعفران در دوزهای mg/kg ۱۰۰ یا بیشتر در موشهای ماده C57BL/6 تلقیح شده با pMOG به طور معنی داری از پیشرفت EAE جلوگیری نمودند. عصاره خیسانده آبی زعفران مانع پیشرفت EAE نگردید. نتایج حاصل از این پژوهش حاکی از آن است که زعفران ممکن است با برخورداری از فعالیت ضد التهابی نشانه های EAE را از بین ببرد.
چکیده انگلیسی :
Experimental autoimmune encephalomyelitis (EAE) is an inflammatory demyelinating disease of central nervous system and commonly used as an animal model for multiple sclerosis (MS). Multiple sclerosis is a disabling neurodegenerative disease in human being. Inflammation is a key event in both EAE and MS, which leads to demyelination and subsequent disability. It is shown that Crocus sativus L. stigma (saffron) extracts have anti-inflammatory activity in rats and mice. There are modern pharmacological evidences for anti-tumour, chemopreventive and genoprotective effects as well as radical scavenger properties of saffron extracts or its constituents. We observed that both therapeutic doses of dexamethasone and methanol extracts of saffron stigma at 100 mg/kg and more prevented EAE from progression significantly in pMOG-immunized female C57BL/6 mice. Aqueous maceration extract of Crocus sativus L. did not prevent EAE progression. These results suggest that saffron may attenuate symptoms of EAE by its anti-inflammatory activity. Experimental data analyzed by one-way ANOVA.
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