تاثیر نانو ذره سلنیوم (Nano-Se) و عصاره سبوس برنج بر جوانه زنی و برخی ویژگی-های مورفوفیزیولوژیکی Astragalus adscendens Boissier )

محورهای موضوعی : زیست شناسی سلولی تکوینی گیاهی و جانوری ، تکوین و تمایز ، زیست شناسی میکروارگانیسم

رضا دهقانی بیدگلی ¹ ( گروه مرتع و آبخیزداری دانشکده منابع طبیعی و علوم زمین دانشگاه کاشان )

تاریخ دریافت : 1396/09/09 تاریخ پذیرش : 1397/04/02 تاریخ انتشار : 1397/03/01

کلید واژه: پرایمینگ, جوانه زنی, کلروفیل, محتوی آب نسبی, طول ریشه چه,

چکیده مقاله :

به منظور بررسی اثر پیش تیمار بذور گون گزی با عصاره سبوس برنج عصاره و محلول نانو ذره سلنیوم در مراحل اولیه جوانه زنی، آزمایشی به صورت فاکتوریل در قالب طرح کاملا تصادفی در 4 تکرار در سال 1396 اجرا شد. تیمار های آزمایش شامل پرایمینگ با عصاره سبوس برنج در 4 سطح (صفر به عنوان شاهد، 1/0 ، 2/0 و 5/0 درصد وزنی-حجمی ) و نانو ذره سلنیوم در 4 سطح (صفر به عنوان شاهد، 04/0، 08/0 و 1/0 درصد وزنی-حجمی ) به مدت 2 ساعت در دمای 25 درجه سانتی گراد بودند. نتایج آزمایشات نشان داد عصاره سبوس برنج، نانو ذره سلنیوم و اثر متقابل تیمارها در سطح احتمال %1 بر تمامی صفات مورد مطالعه شامل درصد جوانه‌زنی، طول ریشه‌چه، طول ساقه‌چه، ضریب جوانه‌زنی، محتوای نسبی آب، محتوای کلروفیل a،b و کلروفیل کل معنی دار بود. همچنین استفاده از عصاره سبوس برنج1/0درصد باعث افزایش 15 درصدی طول ریشه‌چه شد، اما با افزایش غلظت عصاره سبوس برنج طول ریشه‌چه و ساقه‌چه نسبت به شاهد کاهش پیدا کردند. همچنین پرایم بذور با عصاره سبوس برنج2/0درصد و نانو ذره سلنیوم08/0درصد باعث افزایش 37 درصدی طول ریشه‌چه شد. بالاترین میزان درصد جوانه‌زنی، محتوای کلروفیل a،b و طول ساقه‌چه با اعمال تیمار 1/0 درصد وزنی-حجمی عصاره سبوس برنج به همراه تیمار 1/0 درصد وزنی-حجمی نانو ذره سلنیوم به دست آمد. همچنین اعمال تیمار های ذکر شده به تنهایی نیز بر صفات مورد مطالعه اثرات مثبت و معنی دار داشتند.

چکیده انگلیسی:

In order to investigate the effect of seeds priming of Astragalus adscendens with Rice bran extract and potassium nitrate (Nano-Se) solution in the early stages of germination, a factorial experiment was conducted in a completely randomized design (CRD) with four replications conducted at the botany Lab of the Department of Biotechnology, University of Kashan, in 2017. Experimental treatments consisted of priming with Rice bran extrac solution at 4 levels (zero as control, 0. 1, 0.2 and 0.5 (percentage of weight - Volume: w/v), and Nano-Se in 4 levels (zero as control, 0.04, 0.08 and 0.1) w/v for 2 hours at 25 ° C. The results of the experiments showed that Rice bran extract, Nano-Se solution and interaction of treatments were significant at the 1 % level on all studied traits, including germination percentage, root length, stem length, germination coefficient, relative content of water, chlorophyll a, b and total chlorophyll content. Also, the use of rice bran extract 0.1% increased the 15%. of root length but with Increasing the concentrations of bran extract root and shoot length decreased compared to control. Also, seeds priming with rice bran extract 0.2% and Nano- Se 0.8% increased the root length 37%. The highest germination percentage, the content of chlorophyll a and b, and stem length were obtained by applying 0.11% w / v Rice bran extract solution with 0.1% w/v of Nano-Se . Also, applying these treatments alone had positive and significant effects on the studied traits.

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