The Effect of Plant Growth Regulators on Callus Induction and Regeneration in Three Chrysanthemum (Chrysanthemum grandiflorum Ramat) Cultivars Under In Vitro Culture Condition
محورهای موضوعی : مجله گیاهان زینتیMohammad Reza Shafiei 1 , Abdollah Hatamzadeh 2 , Pejman Azadi 3 , Habibollah Samizadeh Lahiji 4
1 - Ornamental Plants Research Center (OPRC), Horticultural Sciences Research Institute (HSRI), Agricultural Research, Education and Extension Organization (AREEO), Mahallat, Iran.
2 - Department of Horticultural Science, Faculty of Agriculture Science, University of Guilan, Rasht, Iran
3 - Department of Genetic Engineering, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Agricultural Research, Education and Extension Organization (AREEO), Iran
4 - Department of Agronomy Plant Breeding, Faculty of Agriculture Science, University of Guilan
کلید واژه: tissue culture, auxin, Micropropagation, Benzyl Adenine, explant,
چکیده مقاله :
Chrysanthemums are known as one of the top three flowers in the floriculture industry. This ornamental plant is traditionally propagated by cuttings. The use of in vitro subculture is expanding for plants like chrysanthemums that are propagated by asexual methods. This research evaluated the effect of plant growth regulators on callus induction and regeneration in three chrysanthemum cultivars including ‘Bonfire Yellow’, ‘Rambla’, and ‘Bella Rosa’. For callus generation, the leaf explants were cultivated in the Murashige and Skoog (MS) culture medium modified with with benzyl adenine (1, 2, or 3 mg/L BA) and naphthaleneacetic acid (0.5 or 1 mg/L NAA) in a factorial experiment based on a completely randomized design. The highest rate of regeneration of ‘Bonfire Yellow’(31.25%), ‘Rambla’(25%), and ‘Bella Rosa’ (31.25%) were obtained from the culture media containing 2 mg/L BA + 1 mg/L NAA, 1 mg/L BA + 1 mg/L NAA, and 3 mg/L BA + 0.5 mg/L NAA, respectively.
گل داوودی به‌عنوان یکی از سه گل مهم در صنعت گلکاری شناخته می‌شود. این گل به‌صورت سنتی توسط قلمه تکثیر می‌شود. استفاده از ریزازدیای درون شیشهای در گیاهانی مانند داوودی که با روش غیر جنسی تکثیر می‌شوند رو به گسترش است. این پژوهش جهت بررسی اثر تنظیم کننده‌های رشد گیاهی بر تولید کالوس و میزان باززایی در سه رقم داوودی Bonfire yellow، Rambla و Bella rosa انجام گرفت. ریزنمونه‌های برگی داوودی جهت تولید کالوس و بصورت آزمایش فاکتوریل در قالب طرح کامل تصادفی در محیط کشت موراشیگ و اسکوگ (MS) حاوی 3 درصد ساکارز و 7 گرم در لیتر آگار به همراه مقادیر مختلف تنظیم کننده‌های رشد بنزیل آدنین (1، 2 و 3 میلی گرم در لیتر) و نفتالین استیک اسید (0/5 و 1 میلی گرم در لیتر) کشت شدند. نتایج به دست آمده در سطح 1 درصد آزمون LSD معنیدار بود. محیط کشت حاوی 2 میلیگرم در لیتر BA و 1 میلیگرم در لیتر NAA در رقم Bonfire yellow، محیط کشت حاوی 1 میلیگرم در لیتر BA و 1 میلیگرم در لیتر NAA در مورد رقم Rambla و محیط کشت حاوی 3 میلیگرم در لیتر BA و 0/5 میلیگرم در لیتر NAA در رقم Bella rosa بیشترین باززایی را نشان داد.
Borodulina, I., Trufanova, A., Shevchenko, G., Sokolova, G. and Plaksina, T. 2019. Optimization of clonal micropropagation of chrysanthemum. Ukrainian Journal of Ecology, 9 (4): 676-678.
da Silva, J.A.T. 2003. Chrysanthemum: Advances in tissue culture, cryopreservation, postharvest technology, genetics and transgenic biotechnology. Biotechnology Advances, 21: 715-766.
Deng, Y., Yang, S., Du, X., Dong, B., Ren, L., Fang, W. and Jiang, J. 2017. Establishment of efficient transient transformation system and the regeneration of stable transformants in chrysanthemum. Journal of Nanjing Agricultural University, 40 (1): 48-53. DOI: 10.7685/jnau.201603046
Eman, A. E. , Andrea, T. and Honfi, P. 2022. Chrysanthemum: A comprehensive review on recent developments on in vitro regeneration. Biology , 11(12): 1774. https://doi.org/10.3390/biology11121774
Firgiyanto, R., Rohman, H.F. and Azizah, M. 2023. Effect of modified murashige and skoog medium on chrysanthemum tissue culture. IOP Conference Series: Earth and Environmental Science, Volume 1168, 5th International Conference on Food and Agriculture 05/11/2022 - 07/11/2022 Banyuwangi, Indonesia.
Hahn, E.J., Bae, J.H. and Lee, Y.B. 1998. Growth and leaf surface characteristics of chrysanthemum plantlets in micropropagation and microponic system. Korean Society of Horticultural Science, 39 (6): 838–842.
Hooker, M.P. and Nabors, M.W. 1977. Callus initiation, growth, and organogenesis in sugarbeet (Beta vulgaris L.). Zeitschrift für Pflanzenphysiologie, 84 (3): 237-246.
Ilahi, I.H.S.A.N., Jabeen, M. and Sadaf, S.N. 2007. Rapid clonal propagation of chrysanthemum through embryogenic callus formation. Pakistan Journal of Botany, 39(6): 1945-1952.
Jevremović, S. and Subotić, A. 2018. Micropropagation of chrysanthemum cultivars in Serbia. In: IX International Scientific Agriculture Symposium" AGROSYM 2018", Jahorina, Bosnia and Herzegovina, 4-7 October 2018. Book of Proceedings (pp. 408-413). University of East Sarajevo, Faculty of Agriculture.
Ji, A., Geng, X., Zhang, Y., Yang, H. and Wu. G. 2011. Advances in somatic embryogenesis research of horticultural plants. American Journal of Plant Sciences, 2: 727-732.
Khalili, S., Jafarkhani Kermani, M., Azadi, P. and Kalatejari, S. 2014. Investigating effects of different factors on regeneration efficiency of two commercial cultivars of Chrysanthemum grandiflorum. In: Proceedings of 1st National Ornamental Plants Congress, 21-22 Oct 2014, Karaj, Iran, p. 43. (in Farsi)
Lim, K.B., Kwon, S.J., Lee, S.I., Hwang, Y.J. and Naing, A.H. 2012. Influence of genotype, explant source, and gelling agent on in vitro shoot regeneration of chrysanthemum. Horticulture, Environment, and Biotechnology, 53 (4): 329-335.
Naing, A.H., Jeon, S.M., Han J.S., Lim, S.H., Lim, K.B.L. and Kim, C.K. 2014. Factors influencing in vitro shoot regeneration from leaf segments of chrysanthemum. Comptes Rendus Biologies, 337: 383-390.
Park, S.H., Kim, G.H. and Jeong, B.R. 2007. Adventitious shoot regeneration from cultured petal explants of chrysanthemum. Horticulture, Environment, and Biotechnology, 48 (6): 387-392.
Song, J.Y., Mattson, N.S. and Jeong, B.R. 2011. Efficiency of shoot regeneration from leaf, stem, petiole and petal explants of six cultivars of Chrysanthemum morifolium. Plant Cell, Tissue and Organ Culture, 107: 295-304.
Thangmanee, C. 2012. Mutation induction by gamma irradiation in chrysanthemum [Chrysanthemum x grandiflorum (Ramat.) Kitam.] (Doctoral Dissertation, Prince of Songkla University).
