Evaluation of Cytotoxic and Anti-microbial Activities of the Methanolic Leaf Extracts of Sansevieria zeylanica (L.) Willd. Against the Human Breast Cancer, MDA-MB-231 Cell Lines

محورهای موضوعی : مجله گیاهان زینتی

Somashekara Rajashekara ¹ , Nuguri Chudamani ² , Priya Rautela ³ , Sharvani Hegde ⁴ , ShivaReddy Swaroopa ⁵

1 - Centre for Applied Genetics, Department of Studies in Zoology, Bangalore University, Jnana Bharathi Campus, Off Mysuru Road, Bengaluru 560 056, India
2 - Centre for Applied Genetics, Department of Studies in Zoology, Bangalore University, Jnana Bharathi Campus, Off Mysuru Road, Bengaluru 560 056, India
3 - Centre for Applied Genetics, Department of Studies in Zoology, Bangalore University, Jnana Bharathi Campus, Off Mysuru Road, Bengaluru 560 056, India
4 - Centre for Applied Genetics, Department of Studies in Zoology, Bangalore University, Jnana Bharathi Campus, Off Mysuru Road, Bengaluru 560 056, India
5 - Centre for Applied Genetics, Department of Studies in Zoology, Bangalore University, Jnana Bharathi Campus, Off Mysuru Road, Bengaluru 560 056, India

تاریخ دریافت : 1400/10/17 تاریخ پذیرش : 1400/11/29 تاریخ انتشار : 1400/12/10

کلید واژه: Cytotoxic effect, Anti-microbial activity, MDA-MB-231 cell lines, Methanolic leaf extract,

چکیده مقاله :

Sansevieria zeylanica is a species of xerophytic perennial herbs with a wide range of distribution found in dry tropical and subtropical parts of the world. It is a wonderful medicinal plant and holds the widespread range of beneficial properties. The aim of the study is to determine the cytotoxic activities of the human breast cancer - MDA-MB-231 cell lines and to evaluate the anti-microbial activities by employing the methanolic leaf extracts of S. zeylanica plants. The main experiment was to determine the susceptibility of a microbial organisms against the methanolic leaf extracts of S. zeylanica. The percentage growth inhibition was calculated and concentration of sample to inhibit cell growth by 50 % (IC50) values was generated for the percentage viability. The leaf extracts produced from S. zeylanica plants showed a cytotoxic effect against the human breast cancer - MDA-MB-231 cell lines. The IC50 value of 1167.78 µg/ ml was obtained for the MDA-MB-231 cell lines after the exposure of 24 h treatment. The increase in cell death of MDA-MB-231 cell lines with an increase in the concentration indicates that the methanolic leaf extract of S. zeylanica plants was found active. For the first time, we made an attempt to examine the biological properties of methanolic leaf extracts from S. zeylanica plants with reference to their wide applications. The results obtained support for the therapeutic applications and this acts as an anti-microbial agent. These studies showed the results from the phytochemical screening, anti-microbial and cytotoxic activities of S. zeylanica indicating that this plant as a “natural herbal source”. This can be used for its pharmaceutical and pharmacological applications in a large-scale industry.

چکیده انگلیسی:

گیاه Sansevieria zeylanica یک گونه علفی چند ساله مناطق خشک است که در قسمت­های گرمسیر و نیمه گرمسیر دنیا پراکندگی دارد. این گیاه، یک گیاه شگفت­انگیز دارویی است و مزایای زیادی دارد. هدف این مطالعه تعیین فعالیت­های سمیت سلولی در سرطان سینه در انسان روی لاین­های سلولی MDA-MB-231 و نیز ارزیابی خاصیت ضد میکروبی با استفاده از عصاره برگ متانولی گیاه S. zeylanica است. آزمایش اصلی تعیین حساسیت میکروب­ها در برابر عصاره­های متانولی برگ گیاه S. zeylanica است. درصد بازدارندگی رشد اندازه­گیری شد و غلظت نمونه برای ممانعت از رشد سلول تا 50 درصد (IC50) برای درصد زنده­مانی تعیین شد. عصاره­های برگی بدست آمده از گیاه S. zeylanica روی لاین­های سلولیMDA-MB-231  سرطان سینه در انسان اثر سمیت سلولی نشان دادند. شاخص IC50 (78/1167 میکروگرم در میلی­لیتر) پس از تیمار 24 ساعته روی این لاین­ها بدست آمد. افزایش مرگ سلولی روی لاین­های MDA-MB-231 با افزایش غلظت عصاره نشان می­دهد که عصاره متانولی برگ این گیاه فعال است. برای اولین بار، ما تلاش کردیم تا خصوصیات بیولوژیکی عصاره برگ متانولی گیاه S. zeylanica را ارزیابی کنیم. نتایج حاصل کاربرد درمانی را تایید کرد و می­تواند برای کاربرد وسیع به­عنوان ماده­ای ضد میکروب، مدرک معتبری باشد. در این مطالعات نتایج غربال­گری فعالیت­های فایتوشیمیایی، ضد میکروبی و سمیت گیاه S. zeylanica تایید کرد که این گیاه می­تواند به­عنوان منبع گیاهی طبیعی مورد استفاده قرار گیرد. این گیاه می­تواند برای مصارف دارویی و داروشناسی در مقیاس وسیع و بصورت صنعتی استفاده شود.

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