Morphological and Physiological Responses of Euonymus (Euonymus japonicas) to Exogenous Paclobutrazol under Salinity Stress
محورهای موضوعی : مجله گیاهان زینتی
Halimeh Zorman
1
(
M.S. Student, Department of Horticultural Sciences, Faculty of Crop Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
)
Mahnaz Karimi
2
(
Assistant Professor, Department of Horticultural Sciences, Faculty of Crop Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
)
کلید واژه: Electrolyte leakage, Enzyme activity, Height, Shoot fresh weight,
چکیده مقاله :
Paclobutrazol (PBZ) is one of the most important triazole compounds. This compound increases plant tolerance to environmental stresses. The present study aimed to investigate the effects of PBZ on morphological and biochemical traits of euonymus (Euonymus japonicas) exposed to NaCl stress. Plants were treated with three levels of PBZ (0, 500 and 1000 mg L-1) and four levels of NaCl (0, 50, 100, 150 mM). Morphological characteristics, electrolyte leakage (EL), chlorophyll content, and antioxidant enzyme activity were changed by the treatment with PBZ and NaCl stress. As compared to the control plants, PBZ at the rates of 1000 and 500 mg L-1 decreased height by 22.8% and 10.44%, respectively. The highest number of lateral shoots was recorded at 1000 mg L-1 PBZ (no salt treatment). The interaction of salinity and PBZ was significant for shoot fresh weight. At 150 mM NaCl + 1000 mg L-1 PBZ, shoot fresh weight was increased by 30% compared to 150 mM NaCl. The PBZ-treated plants had highersuperoxide dismutase (SOD) activity than those not treated with PBZ. PBZ at the rate of 1000 mg L-1 was the best treatment for increasing the activity of this enzyme. PBZ mitigated the salinity stress and reduced EL of euonymus. Compared with 150 mM NaCl alone, EL was decreased by 24.4% in the plants treated with 1000 mg L-1 PBZ + 150 mM NaCl. According to the results, it can be concluded that PBZ can be effective in improving the tolerance of euonymus to 150 mM NaCl.
پاکلوبوترازول یکی از مهمترین ترکیبات تریازولی میباشد. این ماده تحمل گیاه را در برابر تنشهای محیطی افزایش می‌دهد. این مطالعه با هدف بررسی تأثیر پاکلوبوترازول بر صفات مورفولوژیکی و بیوشیمیایی شمشاد (Euonymus japonicas) تحت تنش شوری انجام گرفت. گیاهان ابتدا با پاکلوبوترازول (0، 500 و 1000 میلیگرم در لیتر) تیمار شدند و سپس تحت تنش شوری (0 ، 50 ، 100 ، 150 میلیمولار) قرار گرفتند. خصوصیات مورفولوژیکی، نشت الکترولیت، محتوای کلروفیل و فعالیت آنزیمهای آنتیاکسیدانی با تیمار پاکلوبوترازول و تحت تنش نمک تغییر یافت. در مقایسه با گیاهان شاهد، در 1000 و 500 میلیگرم پاکلوبوترازول، بهترتیب کاهش ارتفاع 8/22 و 44/10 درصد مشاهده شد. بیشترین تعداد شاخساره جانبی در 1000 میلیگرم در لیتر پاکلوبوترازول بدون تنش شوری ثبت شد. اثر متقابل شوری و پاکلوبوترازول بر وزن تر اندام هوایی معنیدار بود. در غلظت 150 میلی مولار نمک + 1000 میلیگرم در لیتر پاکلوبوترازول ، وزن تر اندام هوایی 30٪ در مقایسه با 150 میلیمولار نمک (بدون تیمار پاکلوبوترازول) افزایش نشان داد. در گیاهان تیمار شده با پاکلوبوترازول فعالیت آنزیم سوپراکسید دیسموتاز نسبت به گیاهان بدون تیمار با پاکلوبوترازول بیشتر بود. در مقایسه با 150 میلیمولار کلرید سدیم (بدون تیمار پاکلوبوترازول)، هدایت الکتریکی 4/24 درصد، در 1000 میلیگرم در لیتر پاکلوبوترازول + 150 میلیمولار کلرید سدیم کاهش یافت. با توجه به نتایج این مطالعه میتوان بیان کرد که تیمار پاکلوبوترازول میتواند تنش شوری (150 میلی مولار) را در گیاهان شمشاد کاهش دهد.
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