Effect of Magnetite Nanoparticles on Vegetative Growth, Physiological Parameters and Iron Uptake in Chrysanthemum (Chrysanthemum morifolium) ‘Salvador’
محورهای موضوعی : مجله گیاهان زینتی
Seyed Mohammad Banijamali
1
,
Mohammad Feizian
2
,
Afsaneh Alinejadian Bidabadi
3
,
Ebrahim Mehdipour
4
1 - Ornamental Plants Research Center, Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Mahallat, Iran | Soil Science Department, Faculty of Agriculture, Lorestan University, Khoramabad, Iran
2 - Soil Science Department, Faculty of Agriculture, Lorestan University, Khoramabad, Iran
3 - Soil Science Department, Faculty of Agriculture, Lorestan University, Khoramabad, Iran
4 - Chemistry Department, Faculty of Science, Lorestan University, Khoramabad, Iran
کلید واژه: Chlorophyll, Humic acid, Iron Oxide, Iron chelate, Fe3O4/HA,
چکیده مقاله :
Despite the increasing rate of nanoparticles (NPS) production and their application in agriculture, few studies have focused on their effect on plant growth. So, the present research was conducted in laboratory and greenhouse conditions. First, superparamagnetic iron oxide nanoparticles (SPIONS) with a humic acid coating (Fe3O4/HA) were synthesized in laboratory conditions by the chemical coprecipitation method. The effectiveness of the synthesized nanoparticles in vegetative growth and nutrients uptake of chrysanthemum cut flower (Chrysanthemum morifolium) were evaluated in greenhouse conditions with four replications in a completely randomized design. The treatments consisted of 10, 20 and 40 mg/L of pure Fe from the source of Fe3O4/HA NPS and 1.4 mg/L of pure Fe from two sources of iron chelates which contained Fe-EDTA(Fe-Ethylenediaminetetraaceticacid) and Fe-EDDHA [Fe-ethylenediaminedi(O-hydroxy phenylaceticacid)] were considered as control treatments in the open hydroponic cultivation system. The results of the laboratory experiment indicated that the synthesis of Fe3O4/HA by the chemical coprecipitation method led to the production of nanoparticles with an average diameter of 8.38 nm and superparamagnetic properties. The greenhouse experiment demonstrated that the application of Fe3O4/HA significantly increased Fe uptake, chlorophyll and vegetative growth of the plants versus the control treatments. The highest rates of Fe, N, P, K, Ca, Mg, Mn, and B uptake were observed at the NP rate of 20 mg/L. The branch number per plant, stem height, and total dry weight of the plants were significantly increased by 25, 38, and 39.5% versus the treatment of Fe-EDTA and by 50, 36, and 48% versus the treatment of Fe-EDDHA, respectively. It is concluded that magnetite NPs with a humic acid coating resolved Fe deficiency and increased chrysanthemum growth.
علیرغم تولید روزافزون نانوذرات(NPS) و کاربرد آنها در کشاورزی، بررسیهای محدودی در خصوص تاثیر این مواد بر رشد گیاهان انجام گرفته است. بدینمنظور، این پژوهش در شرایط آزمایشگاهی و گلخانه‌ای به اجرا گذاشته شد. ابتدا سنتز نانوذرات سوپرپارامغناطیسی اکسید آهن مگنتیت (SPIONS) با پوشش اسید هیومیک (Fe3O4/HA) در شرایط آزمایشگاهی به روش هم‌رسوبی انجام شد. آزمون اثر بخشی نانو ذرات سنتز شده بر رشد رویشی و جذب عناصر غذایی داوودی Chrysanthemum morifolium) ) شاخه بریده در شرایط گلخانه‌ای در قالب طرح کاملا تصادفی در چهار تکرار مورد ارزیابی قرار گرفت. تیمارها شامل غلظتهای 10، 20 و 40 میلی‌گرم بر لیتر آهن خالص از منبع Fe3O4/HA NPS و همچنین غلظت 4/1 میلی‌گرم بر لیتر آهن خالص از دو منبع کلات آهن ( Fe-EDTAوFe-EDDHA) بهعنوان تیمارهای شاهد در سیستم کشت هیدروپونیک باز در نظر گرفته شد. نتایج آزمایشگاهی نشان داد سنتز نانوذرات مگنتیت با پوشش اسید هیومیک بهروش هم‌رسوبی منتج به تولید نانوذراتی با قطر میانگین 38/8 نانومتر و با ویژگی مواد سوپرپارامغناطیس شد. آزمایش گلخانه‌ای مشخص کرد کاربرد Fe3O4/HA موجب افزایش معنی‌دار جذب کل آهن، کلروفیل و رشد رویشی گیاهان نسبت به تیمارهای شاهد شد. بیشترین مقدار جذب کل آهن، نیتروژن، فسفر، پتاسیم، کلسیم، منیزیم، منگنز و بور در غلظت ۲۰ میلی‌گرم در لیتر نانوذرات مشاهده شد. تعداد شاخه در بوته، ارتفاع شاخه و وزن خشک کل بوته بهترتیب 25، 38 و 5/39 درصد در تیمارFe-EDTA و50، 36 و 48 درصد در تیمار Fe-EDDHA افزایش معنی‌دار یافت. نتایج این بررسی نشان داد نانو ذرات مگنتیت با پوشش اسید هیومیک موجب رفع کمبود آهن و افزایش رشد داوودی شد.
Albano, J.P. and Miller, W.B. 2001. Ferric ethylenediamine tetraacetic acid (FE-EDTA) photodegradation in commercially produced soluble fertilizers. Horticulture Technology, 11 (2): 265-267.
Asadifard, R., Tilki, R., Rangbar, M., Dini, M., Arab, A., Gajavand, M. and Moradi, A. 2005. Introduction to Nanotechnology Laboratory Equipment: Measurement and characterization. Nanotechnology Laboratory of Iran. Secretariat of the Headquarters for Nanotechnology Development. Science and Technology, Iran. 158 p.
Atak, C., Celik, O., Olgun, A., Alikamanoglu, S. and Rzakoulieva, A. 2007. Effect of magnetic field on peroxidase activities of soybean tissue culture. Biotechnology and Biotechnological Equipment, 21 (2): 166 p.
Ball, P. 2002. Natural strategies for the molecular engineer. Nanotechnology, 13 (5): 15-28.
Bates, L.S., Walrow, R.P. and Teare, I.D. 1973. Rapid determination of free proline for water stress studies. Plant Soil, 39: 205-208.
Borlotti, A., Vigani, G. and Zocchi, G. 2012. Iron deficiency affects nitrogen metabolism in cucumber (Cucumis sativus L.) plants. Plant Biology, 12: 189-204.
Bremmer, J.M. and Malvancy, C.S. 1982. Total nitrogen. P. 595-623. In: A.L. Page et al. (ed.). Methods of soil analysis. 2nd ed. Part 2. American Society of Agronomy, U.S.A.
Briat, J.F. and Lobreaux, S. 1997 Iron transport and storage in plants. Trends in Plant Science, 2: 187-193.
Bucak, S., Yavuzturk, B. and Sezer, A.D. 2012. Magnetic nanoparticles: Synthesis, surface modifications and application in drug delivery. In: A.D. Sezer (ed), Recent Advances in Novel Drug Carrier Systems, 165-200.
Chapman, H.D. and Pratt, P.F. 1961. Methods of analysis for soils, plants and waters. University of California, Division of Agricultural Sciences, P. 169-171.
Chekli, L., Phuntsho, S., Tijing, L.D., Zhou, J.L., Kim, J.H. and Shon, H. K. 2014. Stability of Fe-oxide nanoparticles coated with natural organic matter under relevant environmental conditions. Water Science and Technology, 70 (12): 2040-2046. doi: 10.2166/wst.2014.454.
Chekanova, A.E., Sorkina, T.A., Dubov, A.L., Nikiforov, V.N., Davydova, G.A., Selezneva, I.I., Goodilin, E.A., Dubov, A.L., Trusov, L.A., Korolev, V.V., Aref, I.M., Perminova, I.V. and Tretyakov, Y.D. 2009. New environmental nontoxic agents for the preparation of core-shell magnetic nanoparticles. Mendeleev Communications, 19 (2): 72-74.
Chen, Z., Yin, G., Zhou, Y., Zhang, Y., Song, L., Song, M., Hu, S. and Gu, N. 2012. Dual enzyme-like activities of iron oxide nanoparticles and their implication for diminishing cytotoxicity. ACS Publications, 6 (5): 4001– 4012. https://doi.org/10.1021/nn300291r.
Cifuentes, Z., Custardoy, L., Fuente, J.M., Marquina, C., Ibarra, M.R., Rubiales, D. and Perez-de-Luque, A. 2010. Absorption and translocation to the aerial part of magnetic carbon-coated nanoparticles through the root of different crop plants. Journal of Nanobiotechnology, 8 (26): 1-8. doi.org/10.1186/1477-3155-8-26.
Denre, M., Ghanti, S. and Sarkar, K. 2014. Effect of humic acid application on accumulation of mineral nutrition and pungency in garlic (Allium sativum L.). International Journal of Biotechnology and Molecular Biology Research, 5 (2): 7-12.
Derosa, M.C., Monreal, C., Schnitzer, M., Walsh, R. and Sultan, Y. 2010. Nanotechnology in fertilizers. Nature Nanotechnology, 5:91.
Dickson, D., Liu, G., Li, C., Tachiev, G. and Cai, Y. 2012. Dispersion and stability of bare hematite nanoparticles: Effect of dispersion tools, nanoparticle concentration, humic acid and ionic strength. Science of the Total Environment, 419: 170–177.
Dufkova, V. 1984. EDTA in algal culture media. Archiv fur Hydrobiologie, 37: 479−492.
Fang, M., Strom, V., Olsson, R.T., Belova, L. and Rao, K.V. 2012. Particle size and magnetic properties dependence on growth temperature for rapid mixed co-precipitated magnetite nanoparticles. Nanotechnology, 23: 9 pp. doi:10.1088/0957 -4484/23/14/145601.
Fonteno, W.C. and Bilderback, T.E. 1993. Impact of hydrogel on physical properties of coarse-structured horticultural substrates. Journal of the American Society, 118 (2): 217-222.
Friedly, J., Kent, D. and Davis, J. 2002. Simulation of the mobility of metal−EDTA complexes in groundwater: The influence of contaminant metals. Science and Engineering Journal Abbreviations, 36 (3): 355−363.
Ghafariyan, M.H., Malakouti, M.J., Dadpour, M.R., Stroeve, P. and Mahmoudi, M. 2013. Effects of magnetite nanoparticles on soybean chlorophyll. Journal of Science and Technology, 47: 10645−10652.
Guerinot, M.L. 2001. Improving rice yields–ironing out the details. Nature Biotechnology, 19: 417–418.
Hajdua, A., Illes, E., Tombacz, E. and Borbath, I. 2009. Surface charging polyanionic coating and colloid stability of magnetite nanoparticles. Colloids and surfaces A: Physicochem. Journal of Engineering Aspects, 347: 104–108.
Hu, J.D., Zevi, Y., Kou, X.M., Xiao, J., Wang, X.J. and Jin, Y. 2010. Effect of dissolved organic matter on the stability of magnetite nanoparticles under different pH and ionic strength conditions. Science of the Total Environment, 408 (16): 3477–89.
Illes, E. and Tombacz, E. 2006. The effect of humic acid adsorption on pH-dependent surface charging and aggregation of magnetite nanoparticles. Journal of Colloid and Interface Science, 295 (1): 115–123.
Jalali, M., Ghanati, F. and Modarres-Sanavi, A.M. 2016. Effect of Fe3O4 nanoparticles and iron chelate on the antioxidant capacity and nutritional value of soil-cultivated maize (Zea mays) plants. Crop and Pasture Science, 67: 621–628.
Jalali, M., Ghanati, F., Modarres-Sanavi, A.M. and Khoshgoftarmanesh, A.H. 2017. Physiological effects of repeated foliar application of magnetite nanoparticles on maize plants. Journal of Agronomy and Crop Science, 1: 1–10. https://doi.org/10. 1111/jac.12208.
Kole, C., Kole, P., Randunu, K.M., Choudhary, P., Podila, R., Ke, P.C., Rao, A. and Marcus, R.K. 2013. Nanobiotechnology can boost crop production and quality: First evidence from increased plant biomass, fruit yield and phytomedicine content in bitter melon (Momordica charantia). Biotechnology, 13: 37-47.
Kong, H., Song, J. and Jang, J. 2010. One-step fabrication of C-Fe2O3/polyrhodanine magnetic nanoparticles using in-situ chemical oxidation polymerization and their anti bacterial properties. Chemical Communications, 46: 6735–6737.
Kosegarten, H.U., Hoffmann, H. and Menge, K. 1999. Apoplastic pH and Fe3+ reduction in intact sunflower leaves. Journal of Plant Physiology, 121: 1069–1079.
Kosegarten, H. and Koyro, H.W. 2001. Apoplastic accumulation of iron in the epidermis of maize (Zea mays) roots grown in calcareous soil. Physiologia Plantarum, 113: 515–522.
Li, J., Hu, J., Ma, C., Wang, Y., Wu, C. and Huang, J. 2016. Uptake, translocation and physiological effects of magnetic iron oxide (γ-Fe2 O3) nanoparticles in corn (Zea mays L.). Chemosphere, 159: 326–334. https://doi.org/10.1016/j.chemosphere. 2016.05.083.
Liu, X.M., Zhang, F.D., Feng, Z.B., Zhang, S.Q., He, X.S., Wang, R.F. and Wang, Y.J. 2005. Effects of nano-ferric oxide on the growth and nutrients absorption of peanut. Plant Nutrition and Fertilizer Science, 11: 551-555.
Lu, S., Su, W., Li, H. and Guo, H. 2009. Abscisic acid improves drought tolerance of triploid bermudagrass and involves H2O2- and NO-induced antioxidant enzyme activities. Plant Physiology and Biochemistry, 47: 132–138.
Maathuis, F.J.M. 2013. Plant mineral nutrients, methods and protocols. Department of Biology, University of York, UK. Springer, New York Heidelberg Dordrecht London. 297 p.
Maity, D. and Agrawal, D.C. 2007. Synthesis of iron oxide nanoparticles under oxidizing environment and their stabilization in aqueous and non-aqueous media. Journal of Magnetism and Magnetic Materials, 308: 46–55.
Marshner, H. 2012 Marschner's mineral nutrition of higher plants. Third Edition. Publication of Elsevier, Oxford, 649 p.
Means, J.L., Crerar, D.A. and Duguid, J.O. 1978. Migration of radioactive wastes: Radionuclide mobilization by complexing agents. Science, 200 (4349): 1477−1481.
Monreal, C.M., Derosa, M., Mallubhotla, S.C., Bindraban, P.S. and Dimkpa, C. 2015. The application of nanotechnology for micronutrients in soil-plant systems. VFRC Report 2015/3. Virtual Fertilizer Research Center, Washington, D.C. 44 pp.
Mukherjee, A., Peralta-Videa, J.R., Gardea-Torresdey, J. and White, J.C. 2016. Effects and uptake of nanoparticles in plants. P.386-408. In: B. Xing, C.D. Vecitis and N. Senesi (eds.), Engineered Nanoparticles and the Environment: Biophysicochemical Processes and Toxicity, 1st ed. John Wiley and Sons, New York.
Nel, A., Xia, T., Madler, L. and Li, N. 2006. Toxic potential of materials at the nanolevel. Science, 311 (5): 622-627.
Niu, H., Zhang, D., Zhang, S., Zhang, X., Meng, Z. and Cai, Y. 2011. Humic acid coated Fe3O4 magnetic nanoparticles as highly efficient Fenton-like catalyst for complete mineralization of sulfathiazole. Journal of Hazardous Materials, 190 (1-3): 559-565.
Nowack, B. 2002. Environmental chemistry of amino polycarboxylate chelating agents. Environmental Science and Technology, 36 (19): 4009−4016.
Nyerges, E.I. 2005. Interaction of humic acid with magnetite nanoparticles: from soils to magnetic fluids. Thesis of Ph.D. Dissertation. Department of Colloid Chemistry, University of Szeged, Szeged.
Pariona, N., Martinez, A.I., Hernandez-Flores, H. and Clark-Tapia, R. 2016. Effect of magnetite nanoparticles on the germination and early growth of Quercus macdougallii. Science of the Total Environment. http://dx.doi.org/10.1016/j. scitotenv, 2016.09.128.
Peng, L., Qin, P., Lei, M., Zeng, Q., Song, H., Yang, J., Shao, J., Liao, B. and Gu, J. 2012. Modifying Fe3O4 nanoparticles with humic acid for removal of Rhodamine B in water. Journal of Hazardous Materials. Elsevier. https://doi.org/10.1016/j. jhazmat.2012.01.011.
Rico, C.M., Majumdar, S., Duarte-Gardea, M., Peralta-Videa, J.R. and Gardea-Torresdey, J.L. 2011. Interaction of nanoparticles with edible plants and their possible implications in the food chain. Journal of Agricultural and Food Chemistry, 59: 3485-3498.
Roosta, H.R., Jalali, M. and Vakili-Shahrbabakib, S.M.A. 2015. Effect of nano Fe-chelate, Fe-EDDHA and FeSO4 on vegetative growth, physiological parameters and some nutrient elements concentrations of four varieties of lettuce (Lactuca sativaL.) in NFT system. Journal of Plant Nutrition, 38 (14): 2176-2184.
Rui, M., Ma, C., Hao, Y., Guo, J., Rui, Y., Tang, X., Zhao, Q., Fan, X., Zhang, Z., Hou, T. and Zhu, S. 2016. Iron oxide nanoparticles as a potential iron fertilizer for peanut (Arachis hypogaea). Frontiers in Plant Science, 7: 1-10. doi: 10.3389/fpls. 2016.00815.
Shafiee-Masouleh, S.S., Hatamzadeh, A., Samizadeh, H. and Rad-Moghadam, K. 2014. Enlarging bulblet by magnetic and chelating structures of nano-chitosan as supplementary fertilizer in Lilium. Horticulture, Environment, and Biotechnology, 55 (6): 437-444.
Shahrakizade, M., Gholamalizadeh-Ahangar, A. and Mir, N. 2015. EDTA-coated Fe3O4 nanoparticles: A novel biocompatible fertilizer for improving agronomic traits of sunflower (Helianthus Annuus). Journal of Nanostructures, 5 (2): 117-127.
Taiz, L., Zeiger, E., Moller, I.M. and Murphy, A. 2015. Plant physiology and development. Sunderland, MA: Sinauer Associates.
Tang, S.C. and Lo, I.M. 2013. Review magnetic nanoparticles: Essential factors for sustainable environmental applications. Water Research, 47: 2613-2632.
Taran, M., Amoabediny, G. and Kashanian, F. 2016. The effect of coating on the toxicity of magnetic nanoparticles. Nanotechnology Monthly, 10: 21-27. (In Farsi).
Taweesak, V., Abdullah, T.L., Hassan, S.A., Kamarulzaman, N.H. and Yusoff, W.A.W. 2014. Growth and flowering responses of cut chrysanthemum grown under restricted root volume to irrigation frequency. The Scientific World Journal, Article ID254867, 1-6.http://dx.doi.org/10.1155/2014/254867.
Vione, D., Merlo, F., Marino, V. and Minero, C. 2004. Effect of humic acids on the Fenton degradation of phenol. Environmental Chemistry Letters Journal, 2: 129-133.
Volaire, F., Thomas, H. and Lelievre, F. 1998. Survival and recovery of perennial forage grasses under prolonged Mediterranean drought: I. Growth, death, water relations and solute content in herbage and stubble. New Phytologist, 140: 439–449.
Wang, P., Lombi, E., Zhao, F. and Kopittke, P.M. 2016. Review nanotechnology: A new opportunity in plant sciences. Trends in Plant Science, 8: 699-712.
Wolf, B. 1971. The determination of boron in soil extracts, plant materials, composts, manures, water and nutrient solutions. Communications in Soil Science and Plant Analysis, 2 (5): 363-374. doi: 10.1080/00103627109366326.
Wu, H., Yin, J., Wamer, W.G., Zeng, M. and Lo, Y.M. 2014. Reactive oxygen species-related activities of nano-iron metal and nano-iron oxides. Journal of Food and Drug Analysis, 22: 86-94.
Ylivainio, K. 2010. Effects of iron (III) chelates on the solubility of heavy metals in calcareous soils. Environmental Pollution, 158 (10): 3194−3200.
Zahra, Z., Arshad, M., Rafique, R., Mahmood, A., Habib, A., Quazi, I.A. and Khan, S.A. 2015. Metallic nanoparticle (TiO2 and Fe3O4) application modifies rhizosphere phosphorus availability and uptake by Laktucasativa. Journal of Agricultural and Food Chemistry, 63 (31): 6876-82.
Zhu, H., Han, J., Xiao, J.Q. and Jin, Y. 2008. Uptake, translocation, and accumulation of manufactured iron oxide nanoparticles by pumpkin plants. Journal of Environmental Monitoring, 10 (6): 713-717.
