The Effects of Nanosilver, Nanosil and Hydrogen Peroxide on Vase Life Cut Rose (Rosa hybrida) ‘Grand Press Angela’
محورهای موضوعی : مجله گیاهان زینتیMona Shadbash 1 , Fathollah Keshavarzshal 2
1 - Department of Horticulture, Rasht Branch, Islamic Azad University, Rasht, Iran
2 - Guilan Agricultural and Natural Resources Research and Education Center
کلید واژه: Vase solution, Antimicrobial solutions, Prolonged treatment, Vascular obstruction,
چکیده مقاله :
To evaluate the effect of nanosilver (5, 10 and 20 mg l-1), hydrogen peroxide (200, 400 and 600 µM) and Nanosil(200, 400 and 600 µM) as antimicrobial compounds in vase solution of cut rose cv. ‘Grand Press Angela’ flowers, an experiment with 10 treatments and 3 replications was conducted in a completely randomized design. Cut rose flowers were continuously treated with these compounds. The results indicated a significant and positive impact of Nanosil on the measured traits. The highest vase life (13.16 days), maximum solution uptake (0.943 ml g-1 F.W.), the lowest fresh weight loss (6.31 g), the lowest bacterial population in stem end (87.67 Log10 CFU ml-1) and the highest petals carotenoid (17.61 µg g-1 F.W.) were related to the 400 µM Nanosil treatment. Most POD enzyme activities were recorded for the treatment of nanosilver 5 mg l-1 (3.94 nmol g-1 F.W.) and 400 µM Nanosil (3.86 nmol g-1 F.W.). Different concentrations of nanosilver were not successful in keeping vase life and its related traits because of the continuous use of these compounds. Different concentrations of hydrogen peroxide of evaluated characteristics were superior to the control. Overall, treatment with 400 µM Nanosil is recommended as an effective treatment for long-term preservation of cut flowers of roses as it was the best treatment and also had the longest vase life.
به منظور بررسی اثر نانوسیلور (5، 10 و 20 میلیگرم در لیتر)، پراکسید هیدروژن (200، 400 و 600 میکرو مولار) و نانوسیل (200 ، 400 و 600 میکرو مولار) به عنوان ترکیبات ضدمیکروبی در محلول گلجایی گل بریده رز رقم "گراند پرس آنجلا"، آزمایشی با 10 تیمار و 3 تکرار در قالب طرح کاملا تصادفی انجام شد. گلهای بریده رز بصورت ممتد با این ترکیبات تیمار شدند. نتایج بیانگر تاثیر معنیدار و مثبت نانوسیل بر صفات اندازهگیری شده بود. بطوریکه بیشترین عمر گلجایی (16/13 روز)، بیشترین جذب محلول (943/0 میلیلیتر در هر گرم وزن تر)، کمترین کاهش وزن تر (31/6 گرم)، کمترین باکتری ته ساقه (Log 10 CFU ml-1 67/87) و بیشترین کاروتنوئید گلبرگ (61/17 میکروگرم در هر گرم وزن تر) متعلق به تیمار 400 میکرومولار نانوسیل بود. بیشترین فعالیت آنزیم POD برای دو تیمار 5 میلی گرم درلیتر نانوسیلور (94/3 نانومول در هر گرم وزن تر) و 400 میکرومولار نانوسیل (86/3 نانومول در هر گرم وزن تر) ثبت شد. غلظتهای مختلف نانوسیلور در حفظ عمرگلجایی و صفات وابسته به آن موفق نبودند که دلیل آن میتواند استفاده ممتد از این ترکیبات باشد. غلظتهای مختلف پراکسیدهیدروژن نسبت به شاهد در صفات مورد ارزیابی برتر بودند. بطورکلی تیمار 400 میکرومولار نانوسیل به دلیل اینکه در تمامی صفات ارزیابی شده، جزو بهترین تیمارها بود و همچنین بیشترین عمر گلجایی را داشت، بهعنوان تیمار مناسبی جهت نگهداری طولانی مدت گل بریده رز توصیه میشود.
Abdolmaleki, M., Khosh-Khui, M., Eshghi, S. and Ramezanian, A. 2015. Improvement in vase life of cut rose cv ‘Dolce Vita’ by pre harvest foliar application of calcium chloride and salicylic acid. International Journal of Horticultural Science and Technology; 2(1): 55-66.
Abri, F., Ghasemnezhad, M., Grailoo, S. and Hassan Sajedi, R. 2014. The study of physiological and biochemical changes in cut rose cultivars during senescence. Journal of Plant researches, 27(1): 110-120.
Chance, B. and Maehly, S.K. 1955. Assay of catalase and peroxidase. Methods in Enzymology; 2: 764–775.
Chang Li, Z., Li, L. and Guo Quan, X. 2011. The physiological responses of carnation cut flowers to exogenous nitric oxide. Scientia Horticulturae, 127: 424-430.
Chylinski, K.W., Lukaszewska, A. and Kutnik, K. 2007. Drought response of two bedding plants. Acta Physiology Plant, 29: 399-406.
Figueroa, I., Colinas, M.T., Mejia, J. and Ramirez, F. 2005. Postharvest physiological changes in roses of different vase life. Cienciae Investigación Agraria, 32: 167-176.
Gecheva, T., Gadjeva, I., Van Breusegemb, F., Inzeb, D., Dukiandjieva, S., Tonevaa, A. and Minkov, I. 2002. Hydrogen peroxide protects tobacco from oxidative stress by inducing a set of antioxidant enzymes. Cellular and Molecular Life Sciences; 59:708-714.
Goldani, M. and Kamali, M. 2011. Effect of exogenous application of hydrogen peroxide on water deficit stress in glob amaranth (Gomphrena globose L.) and amaranth (Amaranthus tricolor L.). Plant Products Technology (Agricultural Research), 10(2): 65-81.
Hamdollahi, K., Naghshiband Hasani, R. and Zare Haghi, D. 2014. Effect of hydrogen peroxide on vase life and some physiological characteristics of cut rose cv ‘Candy’. Master's thesis, University of Tabriz. (in Persian).
Hashemabadi, D. 2014. Improving the vase life of cut carnation ‘Temo’ (Dianthus caryophyllus L.) flowers by silver thiosulphate and silver nanoparticles. Journal of Crop Production and Processing, 4(12): 223-234.
Hatami, M., Hatamzadeh, A. and Ghasemnezhad, M. 2012. The role of ascorbic acid in the control of the onset of senescence in cut rose (Rosa hybrida cv. Ruyal class). Iranian Journal of Biology, 25(4): 599-605. (in Persian).
Hossini Darvishani, S.S. and Chamani, E. 2013. An investigasion of the possible improvement of cut rose flower cv. ‘Red Old’ longevity employing organic treatments vs. silver thiosulfate. Iranian Journal of Horticultural Sciences, 44(1): 31-41. (in Persian).
In, B.C., Motomura, S., Inamoto, K., Doi, M. and Mori, G. 2007. Multivariente analysis of realation between preharvest environmental factors, postharvest morphological and physiological factors and vase life of cut Asomi Red Roses.Japanese Society for Horticultural Science. 76: 66-72.
Jin, J., Shan, N., Ma, N., Bai, J. and Gao, J. 2006. Regulation of ascorbate peroxidase at the transcript level is involved in tolerance to postharvest water deficit stress in the cut rose (Rosa hybrida L.) cv. Samantha. Postharvest Biology and Technology, 40: 236-243.
Kaya, C., Higges, D. and Kirnak, H. 2001. The effects of high salinity (NaCl) and supplementary phosphorus and potassium on physiology and nutrition development of spinach. Journal of Plant Physiology, 27 (3-4): 47-59.
Khazaei, M., Nabizadeh, R., Naddafi, K., Izanlou, H., Yavari, Z. and Asadi, M. 2012. The effect of "hydrogen peroxide- silver ion complex" on fecal coliform content in aerated lagoon effluent. Journal of Environmental Science and Technology; 4(14): 39-46. (in Persian).
Kim, J.H., Lee, A.K. and Sub, J.K. 2005. Effect of certain pretreatment substances on vase life and physiological character in Lilium spp. Acta Horticulturae, 673: 307-314.
Liao, L.J., Huang, K.L., Chen, W.S. and Cheng, Y.M. 2000. Postharvest life of cut rose flowers as affected by silver thiosulfate and sucrose. Botanical Bulletin Academica Sinica, 41: 299-303.
Liu, J.P., He, S.G., Zhang, Z.Q., Cao, J.P. Lv, P.T., He, S.D., Cheng, G.P. and Joyce, D.C. 2009. nanosilver pulse treatments inhibit stem- end bacteria on cut gerbera cv. ‘Ruikou’ Flowers. Postharvest Biology and Technology, 54:59-62.
Maneerung, T., Tokura, S. and Rujiravant R. 2008. Impregnation of silver nanoparticles into bacterial cellulose for antimicrobial wound dressing. Carbohydrate Polymers, 72, 43-51
Mirdehghan, S.H., Zeidabadi, S. and Roosta, H.R. 2013. Interaction of medicinal essential oils with calcium chloride and silver nitrate on quality and vase life of rose cut flowers. Iranian Journal of Medicinal and Aromatic Plants, 28 (4): 669-683. (in Persian).
Navarro, E., Baun, A., Behra, R., Hartman, N.B., Filser, J., Miao, A.J., Quigg, A., Santschi, P.H. and Sigg, L. 2008. Environmental behavior and ecotoxicity of engineered nanoparticles to algae, plants and fungi. Ecotoxicology; 17: 372-386.
Oraee, T., Asgharzadeh, A., Kiani, M. and Oraee, A. 2011. The role of preservative compounds on number of bacteria on the end of stems and vase solution of cut Gerbera. Journal of Ornamental and Horticultural Plants; 1(3): 161- 166.
Rahmani, A., Seighali, N. and Ebrahimzadeh, H. 2013. A study on peroxidase activity alterations in corms of saffron (Crucus sativus L.) exposed to different H2O2 concentrations and pH measurement during dormancy and waking. New Cellular and Molecular Biotechnology Journal, 3(10):79-84.
Sadeghi, A., Nasibi, F., Farahmand, H. and Sadeghi, J. 2014. Effect of different concentrations of hydrogen peroxide on flower opening, flower abscission andincrement of vase life of tuberosa cut flowers (Polianthes tuberose L.). 1st National Ornamental Plants Congress, 21, 22 Oct, 2014, Iran, Karaj. 1-4. (in Persian).
Solgi, M. and Ghorbanpour, M. 2015. The effects of biological silver nanopatricles on bacterial growth in preservative solutions and increasing vase life of rose cut flowers "White Naomi". Iranian Journal of Horticultural Science, 46 (3):429-439.
Solgi, M., Kafi, M., Taghavi, T.S. and Naderi, R. 2009. Essential oils and silver nanoparticles (SNP) as novel agents to extend vase-life of gerbera (Gerbera jamesoni cv. Dune) flowers. Postharvest Biology and Technology; 53(3):155-158.
Solgi, M., Kafi, M., Taghavi, T.T., Naderi, R., Eyre, J.X. and Joyce, D.C. 2011. Effects of silver nanoparticles (SNP) on Gerbera jamesonii cut flowers. International Journal of Postharvest Technology and Innovation; 2 (3): 274-285.
Tanoua, G., Molassiotis, A., Diamantidis, G. 2009. Hydrogen peroxide-and nitric oxide-induced systemic antioxidant prime-like activity under NaCl-stress and stress-free conditions in citrus plants. J. Plant Physiology, 166: 1904-1913.
Yamane, K., Kawabata, S. and Fujishige, N. 1999. Changes in activities of superoxide dismutase, catalase and peroxidase during senescence of gladiolus florets. Japanese Society for Horticultural Science, 68: 798-802.
Zaky, A.A. 2013. Effect of pre and postharvest treatments on flower longevity of cut rose cv. ‘Grand Prix’. Egyptian Journal of Agricultural Research; 91(3): 1009-1021.
