Influence of Defoliate Pathotype of Verticillium dahliae on Some Physiological and Biochemical Characteristics of Chrysanthemum (Dendranthema grandiflorum (Ramat.) Kitam)
محورهای موضوعی : مجله گیاهان زینتی
seyed Javad Sanei
1
(
Plant protection, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
)
Seyed Esmael Razavi
2
(
Department of Plant Protection, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
)
کلید واژه: Antioxidant enzymes, reactive oxygen species, Wilt, <, i>, Verticillium dahliae<, /i>, ,
چکیده مقاله :
Verticillium dahliae Kleb.is a soilborne pathogen that cause vascular wilt in chrysanthemumplant (Dendranthema grandiflorum (Ramat.) Kitam). The objective of this research was to study the levels of some organic solutes, such as total protein, total soluble sugars, starch and proline, relative water content, RNA level, malondialdehyde and H2O2 contents in leaves of chrysanthemuminoculated with V. dahliae. Changes of these parameters were measured on 0, 10, 20, 30 and 40 days after inoculation by spectrophotometric analysis. No changes in relative water content, RNA, and protein levels and a slight decrease in chlorophyll level were detected within infected leaf tissue before the appearance of visible wilt. The decrease in relative water content coincided with a sharp build up of proline and total soluble sugars in leaves. The leaf starch and protein levels gradually declined in both healthy and infected plants during the time course of the experiment. However, the decrease was more pronounced in infected plants from the third week after inoculation. A high negative correlation was observed between total soluble sugars and starch contents in leaves of diseased plants (r= 0.764, P<0.001). Changes in malondialdehyde and H2O2 concentration occurred in infected plants between 30-40 days after inoculation, while they did not change in the leaves of control plants. These data suggest the psssible role of senescence during the development of Verticillium wilt syndrome in chrysanthemum.
Verticillium dahliae Kleb. از قارچهای بیمارگر خاکزی است که عامل پژمردگی ورتیسیلیومی گیاه داوودی میباشد. در این تحقیق، سطوح برخی از ترکیبات آلی، ازجمله پروتئین کل، قندهای محلول کل، نشاسته، پرولین، محتوای نسبی آب، سطح RNA و مقدار مالون دیآلدهید و H2O2 در برگهای داوودی آلوده به V. dahliae بررسی شد. تغییر پارامترها در روزهای 0، 10، 20، 30 و 40 روز پس از تلقیح با روشهای مبتنی بر رنگسنجی اندازهگیری شد. بر اساس نتایج، قبل از ظهور علائم تغییری در محتوای نسبی آب، RNA و پروتئین مشاهده نشد و تغییر در میزان کلروفیل اندک بود. تغییر در محتوای نسبی آب همراه با تغییرات قابلتوجه در پرولین و قندهای محلول کل برگ همراه بود. مقدار نشاسته و پروتئین بهتدریج در برگهای سالم و آلوده کاهش داشت. اگرچه، این کاهش در گیاهان آلوده از هفته سوم پس از آلودگی قابلتوجه بود. همبستگی منفی بالایی بین قندهای محلول کل و نشاسته در برگهای بیمار مشاهده شد (001/0P<، 764/0=r). تغییرات مالون دیآلدهید و H2O2 در گیاهان آلوده 30 الی 40 روز پس از آلودگی رخ داد، در حالی که غلظت آنها در برگهای شاهد تغییری نداشت. این نتایج نشانگر نقش پیری همراه با پیشرفت بیماری پژمردگی ورتیسیلیومی در داوودی است.
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