The Feasibility Study on the Prediction of Ploidy Levels Base on Pollen Dimensions in Rosa
محورهای موضوعی : مجله گیاهان زینتیToktam Ahmadi 1 , Zahra Karimian 2 , Maryam Jafarkhani Kermani 3
1 - PhD Student, Department of Horticulture, Ferdowsi University of Mashhad, Iran
2 - Assistant Professor, Department of Ornamental Plants, Research Center for Plant Sciences, Ferdowsi University of Mashhad, Iran
3 - Associate Professor, Department of Tissue and Cell Culture, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), P.O.Box. 31535-1897, Karaj, Iran.
کلید واژه: pollen, Estimation, Ploidy Level, <i>Rosa</i>,
چکیده مقاله :
Ploidy level is one of important factors for plant breeders; therefore models that can predict it are very practical. To estimate of ploidy levels in some species, hybrids and cultivars of Rosa, dimensions of over 500 pollens include pollen length, width and area measured at ABRII. The calculations were carried out in several stages. First, Ploidy levels were regressed with pollen dimensions, after that, pollen area was regressed with pollen length and width. Finally the best models from previous step were tested to estimate of pollen level. In the present study, although a highly positive correlation (r2 ≥0.80, except for 2x) was obtained between pollen length and pollen area, but to predict of ploidy level, the correlation between it and pollen demotions was very weak (r2 ≥0.24). Results of test the best models (with highest r2 and lowest SES) for estimating of ploidy level, despite could be used, but because of the strong correlation among them, were not reliable.
یکی از عوامل مهم برای اصلاح‌کنندگان سطح پلوییدی می‌باشد، بنابراین مدل‌هایی که بتوانند این عامل را پیش‌بینی کنند بسیار کاربردی هستند. به منظور برآورد سطوح پلوییدی در برخی گونه‌ها، هیبریدها و ارقام رز، ابعاد بیش از 500 دانه گرده شامل طول، عرض و مساحت دانه گرده در پژوهشکده بیوتکنولوژی کشاورزی ایران (ABRII) اندازه‌گیری شد. محاسبات در چندین مرحله صورت گرفت. در اولین مرحله، سطوح پلوییدی با ابعاد دانه گرده رگرسیون‌گیری شدند، پس از آن مساحت دانه گرده با طول و عرض دانه گرده رگرسیون گرفته شدند. در نهایت بهترین مدل‌های مرحله قبل جهت برآورد سطح پلوییدی، مورد آزمون قرار گرفتند. در مطالعه حاضر، اگرچه همبستگی بالایی بین طول و مساحت دانه گرده (r2 ≥0.80, except for 2x) به دست آمد اما همبستگی بین سطح پلوییدی و ابعاد دانه گرده خیلی ضعیف بود (r2 ≥0.24). نتایج آزمون بهترین مدل‌ها (با بالاترین r2 و پایین‌ترین SES) به منظور برآورد سطح پلوییدی، علی‌رغم قابل استفاده بودن، به دلیل همبستگی قوی بین آن‌ها قابل اعتماد نیست.
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