Effect of Light Combination and Timing of Supplemental Lighting on Growth Characteristics and Flowering of Pansy (Viola × wittrockiana Rose)
محورهای موضوعی : مجله گیاهان زینتیAzadeh Rashidi 1 , Ali Tehranifar 2 , Hossein Nemati 3
1 - Department of Horticultural Science and Landscape, International Campus, Ferdowsi University of Mashhad, Iran
2 - Professor, Department of Horticultural Science and landscape, Ferdowsi University of Mashhad, Iran
3 - Assistant Professor/Department of Horticultural Science and Landscape , Ferdowsi University of Mashhad,Iran
کلید واژه: Photoperiod, Light quality, Day-extension, LEDs, Night-interruption,
چکیده مقاله :
The goal of this study was to examine how different light combinations and timing of supplemental lighting affect the vegetative and reproductive responses of pansy (Viola × wittrockiana Rose). The present study was designed as a factorial experiment based on a completely randomized block design with three replications and two factors of timing of supplemental lighting (night-interruption and day-extension) and light combination treatments consisting of different proportion of blue (B, 467 nm) and red (R, 625 nm) light with high pressure sodium lamp (HPS) serving as control. The light combination treatments (65 mmol m-2 s-1) included 100% R, 85% R: 15% B, 70% R: 30% B, and HPS. Our results showed that the plants exposed to 85% R: 15% B had the highest foliage fresh and dry weights (2.06 g and 0.23 g), stem diameter (1.42 mm), leaf area (44.72 cm2), and leaf number (16.5), and plants exposed to 100% R had the highest root fresh and dry weights (1.63 g and 0.39 g) and height (6.17 cm), respectively. The results showed a significant interaction between light combination and the timing of supplemental lighting on Chl a, Chl b, Chl total, and carotenoids contents and flowering time. Night-interruption supplemental lighting in exposure to 85% R: 15% B led to the highest Chl a (0.86 mg g-1 FW), Chl b (0.52 mg g-1 FW) and Chl total (1.51 mg g-1 FW) contents. The highest carotenoids content (0.69 mg FW) was obtained from night-interruption supplemental lighting under 70% R: 30% B. Night-interruption supplemental lighting was related to the shortest time to flowering (56 days after seed sowing), but it did not differ significantly from day-extension supplemental lighting under 70% R: 30% B.
هدف از این تحقیق بررسی تاثیر ترکیبات مختلف نور و مقطع زمانی کاربرد نور مکمل بر نحوه رشد رویشی و زایشی بنفشه (Viola × wittrockiana Rose) بود. این پژوهش با استفاده از آزمایش فاکتوریل بر پایه طرح بلوک کامل تصادفی با سه تکرار و دو عامل مقطع زمانی کاربرد نور مکمل (شب شکنی و انتهای روز) و ترکیبات متفاوت نور شامل نسبت‌های مختلف نور آبی (طیف 467 نانومتر) و قرمز (625 نانومتر) به همراه نور لامپ پرفشار سدیم به عنوان شاهد انجام گرفت. تیمار ترکیبات نور (شدت 65 میکرومول بر متر مربع بر ثانیه) شامل 100٪ قرمز، 15٪ آبی: 85٪ قرمز، 30٪ آبی:70٪ قرمز و پرفشار سدیم بود. نتایج نشان داد که گیاهان با کاربرد ترکیب نوری 15٪ آبی: 85٪ قرمز بیشترین وزن تر و خشک برگسار (06/2 گرم و 23/0 گرم)، قطر ساقه (42/1 میلی‌متر)، سطح برگ (72/44 سانتی‌متر مربع)، تعداد برگ (5/16) و با کاربرد نور قرمز بیشترین وزن تر و خشک ریشه (63/1گرم و 39/0گرم) و بیشترین ارتفاع (17/6 سانتی‌متر) را داشتند. نتایج بیانگر اثر معنی‌دار برهمکنش مقطع زمانی بکارگیری نور مکمل و ترکیبات نور بر محتویات کلروفیلa، کلروفیلb، کلروفیل کل، کارتنویید و زمان گلدهی بود. کاربرد ترکیب نوری 15٪ آبی: 85٪ قرمز به صورت شب‌شکنی منجر به بیشترین محتویات کلروفیل a (86/0میلی‌گرم در گرم وزن تازه)، کلروفیلb (52/0 میلی‌گرم در گرم وزن تازه) و کلروفیل کل (51/1 میلی‌گرم در گرم وزن تازه) شد. بیشترین محتویات کارتنویید (69/0 میلی‌گرم در گرم وزن تازه) با کاربرد ترکیب نوری 30٪ آبی: 70٪ قرمز به صورت شب‌شکنی و کوتاه‌ترین زمان گلدهی (56 روز بعد از کاشت بذر) با کاربرد ترکیب نوری30% آبی: 70% قرمز به صورت شب‌شکنی و بدون اختلاف معنی‌دار با کاربرد این ترکیب نوری در انتهای روز بدست آمد.
Adams, S.R., Valdes, V.M. and Langton, F.A. 2008. Why does low intensity, long-day lighting promote growth in Petunia, Impatiens, and tomato? Journal of Horticultural Science and Biotechnology, 83 (5): 609–615.
Agarwal, S. and Pandy, V. 2004. Antioxidant enzyme responses to NaCl stress in Cassia angustifolia. Biologia Plantarum, 48: 555-560.
Ahmad, M. and Cashmore, A. 1997. The blue-light receptor cryptochrome 1 shows functional dependence on phytochrome A or phytochrome B in Arabidopsis thaliana. The Plant Journal, 11 (3): 421-427.
Ahmad, M., Grancher, N., Heil, M., Black, R., Giovani, B., Galland, P. and Lardemer, D. 2002. Action spectrum for cryptochrome-dependent hypocotyl growth inhibition in Arabidopsis. Plant Physiology, 129 (2): 774-785.
Anderson, N.O. 2007. Flower breeding and genetics. Springer, 801 pages.
Arnon, D.I. 1949. Copper enzymes in isolated chloroplasts polyphenoloxidase in Beta vulgaris. Plant Physiology, 24:1-15.
Arteca, R. 1995. Plant growth substances: Principles and applications. Springer. US.
Baba-kasai, A., Hara, N. and Takano, M. 2014. Tissue-specific and light-dependent regulation of phytochrome gene expression in rice. Plant, Cell and Environment, 37: 2654–2666.
Bagnall, D.J., King. R.W. and Hangarter, R.P. 1996. Blue-light promotion of flowering is absent in hy4 mutants of Arabidopsis. Planta, 200: 278-280.
Blanchard, M.G. and Runkle, E.S. 2012. Greenhouse energy curtains influenc shoot-tip temperature of new guinea impatiens. HortScience, 47 (4): 483-488.
Bohne, F. and Linden, H. 2002. Regulation of carotenoid biosynthesis genes in response to light in Chlamydomonas reinhardtii. Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1579 (1): 26-34.
Brown, C.S., Schuerger, A.C. and Sager, J.C. 1995. Growth and photomorphogenesis of pepper plants under red light emitting diodes with supplemental blue or far-red lighting. Journal of American Society for Horticultural Science, 120: 808-813.
Chen, A., Li, C., Hu, W., Hau, M.Y., Lin, H., Rockwell, N.C., Martin, S.S., Jernstedt, J.A., Lagarias, J.C. and Dubcovsky, J. 2014. Phytochrome C plays a major role in the acceleration of wheat flowering under long-day photoperiod. Proceeding of the National Academy of Sciences of the United State of America, 111 (28): 10037-10044.
Cope, K.R. and Bugbee, B. 2013. Spectral effects of three types of white light-emitting diodes on plant growth and development absolute versus relative amount of blue light. HortScience, 48 (4): 504-509.
Craig, D.S. and Runkle, E.S. 2012. Using LEDs to qualify the effect of the red to far-red ratio of night-interruption lighting on flowering of photoperiodic crops. ISHS Acta Horticulturae, 956: 179-186.
Currey, J. and Lopez, R.G. 2013. Cutting of Impatient, Pelargunium and Petunia propagated under light-emitting diodes and high-pressure sodium lamp have comparable growth, morphology, gas exchange and post transplant performance. HortScience, 48 (4): 428-434.
Dole, J. and Wilkins, H.F. 2005. Floriculture: principles and species 2nd (2e). Pearson Higher Ed. USA.
Esashi, Y. 1966. The ralation between red and blue or far-red lights in the night interruption of the photoperiodic tuberization in Begonia evansiana. Plant and Cell Physiology, 7 (3): 405-414.
Folta, K.M. and Spalding, E.P. 2001. Unexpected roles for cryptochrome 2 and phototropin revealed by high-resolution analysis of blue light-mediated hypocotyl growth inhibition. Plant Journal, 26: 471-478.
Franklin, K.A. and Whitelam, G.C. 2005. Phytochromes and shade-avoidance responses in plants. Annals of Botany, 96: 169-175
Fu, W., Guomundsson, O., Paglia, G., Herjolfsson, G., Andresson, O., Palsson, B. and Brynjolfsson, S. 2013. Enhancement of carotenoid biosynthesis in the green microalga Dunaliella salina with light emitting diodes and adaptive laboratory evolution. Applied Microbiology and Biotechnology, 97 (6): 2395-2403.
Fukuda, N., Ajima, C., Yukawa, T. and Olsen, J. 2016. Antagonistic action of blue and red light on shoot elongation in petunia depends on gibberellin, but the effects on flowering are not generally linked to gibberellin. Environmental and Experimental Botany, 121: 102-111.
Fukuda, N. and Olsen, J.E. 2011. Effects of light quality under red and blue light emitting diodes on growth and expression of FBP28 in petunia. Acta Horticulturae, 907: 361–366.
Giliberto, L., Perrotta, G., Pallara, P. and Weller, J. 2005. Manipulation of the blue light photoreceptor cryptochrome 2 in tomato affects vegetative development, flowering time and fruit antioxidant content. Plant Physiology, 137 (1): 199-208.
Heo, J., Lee, C., Chakrabarty, D. and Paek, K. 2002. Growth responses of marigold and salvia bedding plants as affected by monochromic or mixture radiation provided by a light emitting diode (LED). Plant Growth Regulation, 38: 225-230.
Hirai, T., Amaki, W. and Watanabe, H. 2006. Action of blue or red monochromatic light on stem internodal growth depends on plant species. Acta Horticulturae, 711: 345-349.
Ho, C.H., Yang, C.M. and Hsiao, C.L. 2012. Effects of night time lighting with specific wavebands on flowering of chrysanthemum. Crop, Environmental and Bioinformatics, 9: 265−277.
Hopkins, W.G. and Huner, N.P.A. 2004. Introduction to plant physiology. John Wily and Sons, Inc., USA.
Islam, M.A., Kuwar, G., Clarke, J., Blystad, D.R., Gislerod, H.R., Olsen, J.E. and Torre, S. 2012. Artificial light from light emitting diodes (LEDs) with a high portion of blue light results in shorter poinsettias compared to high pressure sodium (HPS) lamps. Scientia Horticulturae, 147: 136-143.
Izhaki, A., Swain, S., Tseng, T., Borochov, A., Olszewski, N. and Weiss, D. 2001. The role of SPY and TPR domain in the regulation of gibberellin action throughout the life cycle of Petunia hybrida plants. The Plant Journal, 28 (2): 181-190.
Jeong, S.W., Hogewoning, S.H. and Ieperen, W.V. 2014. Responses of supplemental blue light on flowering and stem extension growth of cut chrysanthemum. Scientia Horticulturae, 165: 69-74.
Johkan, M., Shoji, K., Goto, F., Hashida, S. and Yoshibara, T. 2010. Blue light-emitting diode light irradiation of seedlings improves seedling quality and growth after transplanting in red leaf lettuce. HortScience, 45 (12): 1809–1814.
Kenneth, E. 1992. Light-quality effects on Arabidopsis development. Red, blue and far-red regulation of flowering and morphology. Plant Physiology, 86: 439–444.
Kim, H.H., Heins, R.D. and Carlson, W.H. 2002. Development and flowering of petunia grown in a far-red deficient light environment. Acta Horticulturae, 580: 127-135.
Klein, R.M. 1992. Effect of green light on biological systems. Biolological Reviews, 67: 199-284.
Kohler, K.H., Dorfler, M. and Goring, H. 1980. The influence of light on the cytokinin content of Amaranthus seedlings. Biologia Plantarum, 22 (2): 128-134.
Korner, O. and van Straten, G. 2008. Decision support for dynamic greenhouse climate control strategies. Computers and Electronics in Agriculture, 60: 18–30.
Liorente, B., D''Anfrea, L., Ruiz-Sola, M., Botterweg, E., Pulido, P., Andilla, J., Loza-Alvarez, P. and Concepcion, M. 2015. Tomato fruit carotenoid biosynthesis is adjusted to actual ripening progression by a light-dependent mechanism. The Plant Journal, 85 (1): 107-119.
Liu, X., Xu, Z., Guo, S., Chang, T., Xu, Z. and Tezuka, T. 2012. Regulation of the growth and photosynthesis of cherry tomato seedlings by different light irradiations of light emitting diodes (LED). African Journal of Biotechnology, 11 (22): 6169-6177.
Ma, G., Zhang, L., Kato, M., Yamawaki, K., Kiriiwa, Y., Yahata, M., Ikoma, Y. and Matsumoto, H. 2015. Effect of the combination of ethylene and red LED light irradiation on carotenoid accumulation and carotenogenic gene expression in the flavedo of citrus fruit. Postharvest Biology and Technology, 99: 99-104.
Mattson, N.S. and Erwin, J.E. 2005. The impact of photoperiod and irradiance on flowering of several herbaceous ornamentals. Scientia Horticulturae, 104: 275-292.
Meng, O. and Runkle, E. 2015. Low intensity blue light in night interruptoin lighting does not influence flowering of herbaceous ornamentals. Scientia Horticulturae, 186: 230-238.
Neff, M.M. 2012. Light mediated seed germination: Connecting phytochrome B to gibberellic acid. Developmental Cell, 22: 687-688.
Nissim-Levi, A., Farkash, L., Hamburger, D., Ovadia, R., Forrer, L., Kagan, S. and Oren-Shamir, M. 2008. Light-scattering shade net increases branching and flowering in ornamental pot plants. Journal of Horticultural Science and Biotechnology, 83: 9-14.
Oh, W., Runkel, E.S. and Warner, R.M. 2010. Timing and duration of supplemental lighting during the seedling stage influence quality and flowering in petunia and pansy. HortScience, 45 (9): 1332–1337.
Ohashi-Kaneko, K., Takase, M., Kon, N., Fujiwara, K. and Kurata, K. 2007. Effect of light quality on growth and vegetable quality in leaf lettuce, spinach and komatsuna. Environmental Control in Biology, 45: 189–198.
Pinho, P., Oskari, M., Eino, T. and Lisa, H. 2004. Photobiological aspects of crop plants grown under light emitting diodes. Proceeding CIE expert symposium. LED light sources. Tokyo, Japan, 7-8 June. p. 71-74.
Randall, W.C. and Lopez, R.G. 2014 .Comparison of supplemental lighting from high-pressure sodium Lamps and light-emitting diodes during bedding plant seedling production. HortScience, 49 (5): 589–595.
Randall, W.C. and Lopez, R.G. 2015. Comparison of bedding plantseedling grown under sole-source light-emitting diodes and greenhouse supplemental lighting from LEDs and high pressure sodium lamps. HortScience, 50 (5): 705-713.
Riikonen, J., Kettunen, N., Gritsevich, M., Hakala,T., Sarkka, L. and Tahvonen, R. 2016. Growth and development of Norway spruce and Scots pine seedlings under different light spectra. Environmental and Experimental Botany, 121: 112-120.
Saini, R.S. 2006. Laboratory manual of analytical techniques in horticulture. Agrobios. India
Shimizu, M., Ma, Z. and Douzono, M. 2006. Blue light inhibits stem elongation of chrysanthemum. Acta Horticulturae, 711: 363–368.
Shin, J.H., Jung, H. and Kim, K.S. 2010. Night interruption using light emitting diods (LEDs) promotes flowering of Cyclamen persicum in winter cultivation. Horticulture Environment and Biotecnology, 51 (5): 391-395.
Smith, H. 2000. Phytochromes and light signal perception by plants – an emerging synthesis. Nature, 407: 585–591.
Takemiya, A., Inoue, S., Doi, M. and Kinoshita, T. 2005. Phototropins promote plant growth in response to blue light in low light environments. The Plant Cell, 17: 1120–1127.
Terfa, M.T., Solhaug, K.A., Gislerod, H.R., Olsen, J.E. and Torre, S. 2013. A high proportion of blue light increases the photosynthesis capacity and leaf formation rate of Rosa × hybrida but dose not affect time to flower opening. Physiologia Plantarum, 148: 146-159.
Thomas, B. and Vince-Prue, D. 1996. Photoperiodism in plants, 2nd ed. Academic Press. London.
Wojciechowska, R., Kolton, A., Grochowska, O. and Knop, E. 2016. Nitrate content in Valerianella locusta L. plants is affected by supplemental LED lighting. Scientia Horticulturae, 211: 179-186.
Wollaeger, H.M. and Runkle, E.S. 2014. Growth of Impatiens, Petunia, Salvia, and tomato seedling under blue, green and red light emitting diodes. HortScience, 49 (6): 734-740.
Yorio, N.C., Goins, G.D., Kagie, H.R., Wheeler, R.M. and Sager, J.C. 2001. Improving spinach, radish, and lettuce growth under red light emitting diodes (LEDs) with blue light supplementation. HortScience, 36: 380-383.
