Appraisal of Hematological Indices and Humoral Immunity in Commercial Laying Hens Fed Rations Consisting Cottonseed Meal and Sodium Bentonite

محورهای موضوعی : Camel

A. Gilani ¹ (Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran)
ح. کرمانشاهی ² (Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran)
ا. گلیان ³ (Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran)
ع. طهماسبی ⁴ (Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran)
م. اعمی ازغدی ⁵ (Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran)

تاریخ دریافت : 1391/02/12 تاریخ پذیرش : 1391/07/06 تاریخ انتشار : 1392/09/10

کلید واژه: laying hens, blood components, cottonseed meal, humoral immunity, sodium bentonite,

چکیده مقاله :

The objective of this study was to evaluate the effects of cottonseed meal (CSM) treated with sodium bentonite (SB) on certain blood components and immune response of laying hens. A 3 × 3 factorial arrangement with 3 levels of SB (0, 10, and 20 g/kg of diet) and 3 levels of CSM (0, 100 and 200 g/kg of diet) were used within 9 dietary treatments of 4 replicates each. Nine mash diets were fed to 288 commercial Hy-Line W-36 hens from 51-63 weeks of age. Blood samples were collected at the end of trial to evaluate the blood constituents. The SB, CSM, or their interaction (SB×CSM) did not have any adverse effect on blood cells and activity of serum enzymes. Sheep red blood cells (SRBC) were used as antigen to quantify the antibody response. Two birds per replicate were injected with SRBC at 60 week of age. After 7 and 14 d of SRBC inoculation, blood samples were obtained from the brachial vein of each hen, and total anti-SRBC, IgG and IgM titers were determined. A significant difference of SB, CSM or their interaction was not observed for total antibody response against SRBC inoculation; however, IgG was significantly increased with 20% CSM at 7 d (7.62 vs. 8.45) and with 2% SB at 14 d (2.79 vs. 3.66) after injection of SRBC. The interaction of SB and CSM for IgG was significantly different among dietary treatments and diet with 2% SB and 20% CSM had the highest titer (5 vs. 2.5) at 14 d after injection of SRBC. In conclusion, SB, CSM or their interactions did not affect blood constituents but significantly changed immune response of the birds.

چکیده انگلیسی:

هدف این تحقیق ارزیابی اثرات کنجاله پنبه­دانه عمل­آوری شده با بنتونیت سدیم بر برخی ترکیبات خونی و پاسخ ایمنی مرغ­های تخمگذار تجاری بود. این پژوهش به‌صورت ترتیب فاکتوریل 3 × 3 در قالب طرح کاملاً تصادفی با سه سطح 0، 1 و 2 درصد بنتونیت سدیم و سه سطح 0، 10 و 20 درصد کنجاله پنبه­دانه در جیره انجام شد. این آزمایش با 9 تیمار و 4 تکرار 8 قطعه­ای مرغ تخمگذار هایلاین واریته 36W- از سن51 تا 63 هفتگی اجرا شد. نمونه­های خونی برای آنالیز سلول­ها و ترکیبات در انتهای دوره از سیاهرگ بال به­دست آمدند. بنتونیت سدیم، کنجاله پنبه­دانه و اثر متقابل آنها تاثیر منفی بر سلول­ها و ترکیبات خونی نداشتند. گلبول قرمز گوسفند به‌عنوان آنتی­ژن برای سنجش پاسخ ایمنی استفاده شد که به دو پرنده از هر تکرار در 60 هفتگی تزریق شد. نمونه­های خونی 7 و 14 روز پس از تزریق گلبول­های قرمز گوسفند از هر مرغ گرفته شده و تیتر anti-SRBC،IgG  وIgM  تعیین شدند. هیچ اثر معنی­داری از بنتونیت سدیم، کنجاله پنبه­دانه و اثر متقابل آنها بر کل پادتن تولیدی بر ضد گلبول­های قرمز گوسفند مشاهده نشد، اما IgG با استفاده از میزان20 درصد کنجاله پنبه­دانه در جیره در روز 7 پس از تزریق و با 2 درصد بنتونیت سدیم در 14 روز پس از تزریق به طور معنی­داری افزایش یافت. میزان IgG در مورد اثرات متقابل نیز در تیمار حاوی 2 درصد بنتونیت سدیم و 20 درصد کنجاله پنبه­دانه در مقایسه با تیمار شاهد 14 روز پس از تزریق به طور معنی­داری افزایش یافت. در نتیجه، ترکیبات خونی به­طور معنی­داری تحت تأثیر جیره­های آزمایشی قرار نگرفتند، اما پاسخ ایمنی تغییر کرد.

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