Role of Follicle Stimulating Hormone in the Survival, Activation and Further Growth of in vitro Cultured Sheep Primordial Follicles
محورهای موضوعی : Camelف. اسماعیل زاده 1 , س. م. حسینی 2 , م. حاجیان 3 , ز. نصیری 4 , م. چمنی 5 , م. امین افشار 6 , M.H. Nasr-Esfahani 7
1 - Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Reproduction and Development, Reproductive Biomedicine Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran
3 - Department of Reproduction and Development, Reproductive Biomedicine Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran
4 - Department of Reproduction and Development, Reproductive Biomedicine Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran
5 - Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
6 - Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
7 - Department of Reproduction and Development, Reproductive Biomedicine Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran
کلید واژه: In vitro culture, Ovine, primordial follicles, FSH,
چکیده مقاله :
The aim of the present study was to investigate the effect of follicle stimulating hormone (FSH) on survival, activation and growth of ovine primordial follicles using histological studies. Pieces of ovine ovarian cortex were obtained and cultured for 6 days in Minimum Essential Medium supplemented with or without FSH (50 ng/mL). These fragments were then process to be used for histology compared to each other and non-cultured group. The results showed that the lowest percentage of normal follicles in control medium was observed after 6 days of culture. A higher percentage of growing follicles was observed in presence vs. absence of FSH. Moreover, an increase in diameter of both oocyte and follicle was observed in those fragments that have been cultured in presence of FSH. This study demonstrated that FSH not only maintains the morphological integrity of ovine preantral follicles cultured for 6 days in vitro, but also stimulates the activation of primordial follicles and the growth of activated follicles.
هدف از این مطالعه بررسی اثر هورمون FSH بر بقا، فعال‌سازی و رشد بیشتر کشت آزمایشگاهی فولیکول‌های بدوی گوسفند با استفاده از مطالعات بافت‌شناسی است. قطعات قشری بافت تخمدان در دو گروه کنترل کشت‌شده (محیط کشت MEM) و آزمایشی (محیط کشت پایه+هورمون FSH (غلظت ثابت 50ng/mL )) به مدت شش روز کشت گردید. سپس قطعات کشت نشده و کشت شده برای ارزیابی مورفولوژیکی (در سه گروه) فرآوری شدند. همچنین اثر هورمون FSH بر قطر فولیکول‌ها و اووسیت‌های کشت شده بررسی گردید. نتایج نشان داد که پس از شش روز کشت، کمترین و بیشترین درصد فولیکول‌های نرمال و رشد کرده به ترتیب در گروه‌های کنترل کشت شده و آزمایشی به دست آمد. همچنین بیشترین افزایش قطر اووسیت و فولیکول‌های نرمال در گروه آزمایشی با غلظت ثابت هورمون FSH (50ng/mL ) ملاحظه شد. بنابراین عامل FSH در روند تکوین، تداوم رشد و بقای فولیکول‌های بدوی تخمدان گوسفند نقش مؤثری دارد.
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