Genetic Relationships among Four Saudi Arabian Sheep Populations

محورهای موضوعی : Camel

R.S. Aljumaah ¹ (Department of Animal Production, Food and Agriculture College, King Saud University, Riyadh, 11451, Saudi Arabia)
M.A. Al-Shaikh ² (Department of Animal Production, Food and Agriculture College, King Saud University, Riyadh, 11451, Saudi Arabia)
H. Kibogo ³ (International Livestock Research Institute, Naivasha Road, Nairobi, Kenya)
A. Kwallah ⁴ (International Livestock Research Institute, Naivasha Road, Nairobi, Kenya)
Jianlin Jianlin ⁵ (International Livestock Research Institute, Naivasha Road, Nairobi, Kenya)
O. Hanotte ⁶ (International Livestock Research Institute, Naivasha Road, Nairobi, Kenya)
M.M. Musthafa ⁷ (Department of Animal Production, Food and Agriculture College, King Saud University, Riyadh, 11451, Saudi Arabia)
F.M.M.T. Marikar ⁸ (Department of Animal Production, Food and Agriculture College, King Saud University, Riyadh, 11451, Saudi Arabia)

تاریخ دریافت : 1392/10/08 تاریخ پذیرش : 1393/01/26 تاریخ انتشار : 1393/09/10

کلید واژه: Saudi Arabia, Sheep, Microsatellite, genetic relationship, heterozygosity, phylogenetic tree,

چکیده مقاله :

Four Saudi Arabian indigenous sheep populations including Najdi, Hbsi, Arb, and Naemi were genotyped for 16 microsatellite markers recommended by the food and agriculture organization (FAO). This study aims to provide information on the genetic structure of the breeds analyzed and give indications and proposals for the cross breeding program. All loci were polymorphic in all populations and locus combinations were at Hardy-Weinberg equilibrium except ILSTS044, ILSTS005, MAF209, HUJ616, OARFCB226 and SRCRSP09 (P<0.05). There was substantial genetic variability within sheep populations, with average heterozygosity range of 0.759-0.811 based on expected hetrozygosity. It was observed that the four sheep populations showing the lowest level of inbreeding on the basis of heterozygote deficiency. The lowest genetic distance (0.013) was obtained between Hbsi and Najdi and the highest genetic distance (0.146) between Arb and Hbsi. Estimates of inbreeding coefficient (FIS) were significant for three breeds, except for Arb breeds (P<0.05). The sequence results of the 16 microsatellite markers were sequenced and then phylogenetic tree based on unbiased distances was drawn using MEGA 4. To study the genetic relationships among sheep populations, a principal coordinate analysis (PCA) based on Nei standard distances was performed which indicated a conservation program is needed in these sheep population since most of them are in danger of inbreeding.

چکیده انگلیسی:

چهار جمعیت گوسفند بومی عربستان سعودی، شامل نجدی، حبشی، عرب و نائمی، برای 16 نشانگر ریزماهواره توصیه شده توسط سازمان خواربار و کشاورزی ملل متحد (FAO) تعیین ژنوتیپ شدند. این مطالعه با هدف آگاهی از ساختار ژنتیکی این نژادها و ارائه پیشنهاداتی برای اجرای برنامه آمیخته‌گری بین آنها انجام شد. کلیه جایگاه‌های ژنی در تمامی جمعیت‌ها چندشکل بوده و کلیه جایگاه‌ها به غیر از ILSTS044، ILSTS005، MAF209، HUJ616، OARFCB226 و SRCRSP09 در تعادل هاردی - واینبرگ بودند (05/0>P). تنوع ژنتیکی در این جمعیت‌ها در سطح مناسبی بوده و هتروزیگوسیتی مورد انتظار به طور متوسط بین 759/0 تا 811/0 در نوسان بوده است. با در نظر گرفتن میزان کاهش هتروزیگوسیتی مشخص شد که همخونی در جمعیت‌های مزبور در پایین‌ترین سطح قرار دارد. کمترین فاصله ژنتیکی (013/0) بین جمعیت‌های حبشی و نجدی و بیشترین فاصله ژنتیکی (146/0) بین جمعیت‌های عرب و حبشی مشاهده شد. برآورد ضریب همخونی (FIS) برای همه جمعیت‌ها، به غیر از جمعیت عرب معنی‌دار گردید (05/0>P). نشانگرهای مزبور توالی‌یابی شده و درخت ژنتیکی برمبنای فاصله نااُریب با استفاده از نرم‌افزار MEGA 4 ترسیم گردید. برای مطالعه ارتباط ژنتیکی بین این جمعیت‌های گوسفند، آنالیز مختصات اصلی بر مبنای فاصله استاندارد نِی انجام شد. نتایج حاکی از آن بود که اجرای یک برنامه حفاظت در این جمعیت‌های گوسفند ضرورت دارد. زیرا اکثر آنها در معرض خطر همخونی قرار دارند.

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