Molecular Study on the Exon 2 Region of the Ovis Bone Morphology Protein 15 (BMP-15) Gene in Iranian Bluchi Sheep Breed by PCR-SSCP Technique

محورهای موضوعی : Camel

A. Gholibeikifard ¹ , M. Amin Afshar ² , M. Hosseinpour Mashhadi ³ , H. Mohammadi ⁴

1 - Department of Animal Science, Technical and Vocational University, Tehran, Iran
2 - Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 - Department of Animal Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran
4 - Department of Agriculture, Payame Noor University, Tehran, Iran

تاریخ دریافت : 1392/10/06 تاریخ پذیرش : 1392/12/10 تاریخ انتشار : 1393/09/10

کلید واژه: polymorphism, litter size, BMP-15, FecXL, gene, TGF-ß,

چکیده مقاله :

Litter size is one of the most important economical traits in sheep breeding industry. In addition to minor genes, litter size is under the influence of major genes. Bone morphogenetic protein 15 (BMP-15), a member of the transforming growth factor beta (TGF-ß) superfamily, which is specifically expressed in oocytes, plays a dramatic role in sheep prolificacy. Reported mutations in this gene cause increased ovulation rate and infertility in a dosage-sensitive manner. Six different point mutations have been indicated in the BMP-15 gene of sheep, each having a major effect on prolificacy. The aim of the current study was to investigate the Lacaune (FecXL) mutation in the prolificacy of the Iranian Baluchi sheep breed. Blood samples were collected in ethylenediaminetetraacetic acid(EDTA) coated tubes from jugular vein and genomic DNA was extracted from whole blood samples. Single nucleotide polymorphism of FecXL loci in BMP-15 gene were determined using polymerase chain reactionsingle-strand conformation polymorphism(PCR-SSCP) technique. No evidence of mutation in FecXL was observed, all of which were monomorphic for exon 2 BMP-15 gene.

چکیده انگلیسی:

بالا بودن نرخ تخمک‌گذاری و بره‌زایی یکی از مهمترین عوامل تأثیرگذار بر افزایش کارآیی تولید مثل و به تبع آن افزایش کارآیی اقتصادی در صنعت پرورش گوسفند به حساب می‌آیند. علاوه بر تأثیر ژن‌های کوچک اثر، صفت چند‌قلوزایی تحت تأثیر ژن‌های بزرگ اثر نیز می‌باشد. پروتئین مورفوژنتیک استخوان شماره 15 (BMP15) عضو فوق خانواده فاکتور رشد تغییر شکل دهنده بتا (TGF-β) می‌باشد که به طور خاص در اووسیت بیان شده و نقش تعیین کننده‌ای در باروری گوسفند دارد. گزارش شده جهش‌های مختلف در این ژن با روش حساس به دوز سبب افزایش نرخ تخمک‌گذاری و ناباروری در گوسفند می‌گردد. شش جهش در ژن BMP15 شناسایی شده‌اند که دارای اثر عمده بر روی باروری گوسفند است. هدف این پژوهش بررسی جهش لوکوس FecXL در باروری گوسفند نژاد بلوچی ایرانی بوده است. نمونه‌های خون کامل از وداج گردن و با استفاده از لوله خلاء دار حاوی ماده ضد انعقاد EDTA جمع‌آوری شدند و سپس DNA ژنومی از همه نمونه‌ها استخراج گردید. برای تعیین الگوهای باندی از روش چند شکلی فضایی تک رشته‌ای (SSCP) و همچنین توالی یابی استفاده گردید. نمونه‌های تعیین ژنوتیپ شده در این مطالعه وقوع آلل جهش یافته برای لوکاس FecXL را تأیید نکرد و تمامی نمونه‌ها در هر دو جایگاه از اگزون 2 ژن BMP15 دارای ژنوتیپ مونومورف بودند.

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