Effect of Milk Thistle (Silybum marianum) Seed on Performance, Nutrient Digestibility and Small Intestine Morphology of Broiler Chickens

محورهای موضوعی : Camel

S.A. Khatami ¹ ( Department of Animal Science, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran )
M.D. Shakouri ² ( Department of Animal Science, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran )
A. Mojtahedin ³ ( Department of Animal Science, Faculty of Agriculture and Natural Resources (Moghan), University of Mohaghegh Ardabili, Ardabil, Iran )

تاریخ دریافت : 1402/04/11 تاریخ پذیرش : 1402/07/14 تاریخ انتشار : 1402/09/10

کلید واژه: performance, digestibility, blood parameters, broilers, Milk thistle,

چکیده مقاله :

The present study investigated the effect of different levels of milk thistle (Silybum marianum) seed on performance, nutrient digestibility and small intestine morphology of broiler chickens. For this purpose, 256 broiler chicks (Ross 308) were randomly assigned to four treatments with four replications and 16 chicks in each replication, by employing a completely randomized design. Treatments were included: 1) control, 2) basal diet + 0.2 % milk thistle seed, 3) basal diet + 0.4 % milk thistle seed and 4) basal diet + 0.6 % milk thistle seed. The results showed that the use of different levels of milk thistle increased feed intake compared with the control, but weight gain decreased with increasing levels of milk thistle (P<0.05). The feed conversion ratio increased by adding milk thistle to the diet (P<0.05). The addition of different levels of milk thistle in the diet reduced the digestibility of dry matter and organic matter compared with the control but did not have a significant effect on the digestibility of ether extract and ash. The concentration of serum LDL-c, VLDL-c and triglyceride was decreased by 0.6% milk thistle (P<0.05). The jejunal villus height and villus height to crypt depth significantly decreased using 0.2% milk thistle seed in the diet of broilers (P<0.05). The villus height to crypt depth in the ileum increased by milk thistle seed (P<0.05). In general, the milk thistle had no positive effect on growth performance, digestibility of dry matter and organic matter, and intestinal morphology. Therefore, according to the results of this study, it is not recommended to use milk thistle seed in broiler chickens diet.

منابع و مأخذ:

Ahmad M., Chand N., Khan R.U., Ahmad N., Khattak I. and Naz S. (2020). Dietary supplementation of milk thistle (Silybum marianum): growth performance, oxidative stress, and immune response in natural summer stressed broilers. Trop. Anim. Health Prod. 52, 711-715.

Alheidary A., Rehman Z., Khan R.U. and Tahir M. (2017). anti-aflatoxin activities of milk thistle (Silybum marianum) in broiler. Worlds Poult. Sci. J. 73, 559-566.

AOAC. (2000). Official Methods of Analysis. 17^th Ed. Association of Official Analytical Chemists, Arlington, Washington, DC., USA.

Ashraf S., Shaukat Bhatti A., Kamran Z. and Ahmed F. (2019). Assessment of refined functional carbohydrates as substitutes of antibiotic growth promoters in broilers: Effects on growth performance, immune responses, intestinal microflora and carcass characteristics. Pakistan Vet. J. 39(2), 157-162.

Aviagen. (2019). Ross 308: Broiler Performance Objectives and Nutrition Specifications. Aviagen Ltd., Newbridge, UK.

Banaee M., Sureda A., Mirvaghefi A.R. and Rafei G.R. (2011). Effects of long-term silymarin oral supplementation on the blood biochemical profile of rainbow trout (Oncorhynchus mykiss). Fish Physiol. Biochem. 37, 885-896.

Blevins S., Siegel P.B., Blodgett D.J., Ehrich M., Saunders G.K. and Lewis R.M. (2010). Effects of silymarin on gossypol toxicosis in divergent lines of chickens. Poult. Sci. 89, 1878-1886.

Chambers C.S., Holeˇcková V., Petrásková L., Biedermann D., Valentová K., Buchta M. and Kren V. (2017). The silymarin composition and why does it matter. Food Res. Int. 100, 339-353.

Fenton T.W. and Fenton M. (1979). An improved procedure for the determination of chromic oxide in feed and feces. J. Anim. Sci. 59, 631-634.

Friedewald W.T., Levy R.I. and Fredrickson D.S. (1972). Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin. Chem. 18, 499-502.

Gillessen A. and Hartmut H.J.S. (2020). Silymarin as supportive treatment in liver diseases: A narrative review. Adv. Therapy. 37, 1279-1301.

Hashemi Jabali N.S., Mahdavi A.H., Ansari Mahyari S., Sedghi M. and Akbari Moghaddam Kakhki R.E. (2017). Effect of milk thistle meal on performance, ileal bacterial enumeration, jejunal morphology and blood lipid peroxidation in laying hens fed diets with different levels of metabolizable energy. J. Anim. Physiol. Anim. Nutr. 101, 1-11.

Iji P.A., Hughes R.J., Choct M. and Tivey D.R. (2001). Intestinal structure and function of broiler chickens on wheat-based diets supplemented with a microbial enzyme. Asian-australasian J. Anim. Sci. 14, 54-60.

Jahanian E., Mahdavi A.H., Asgary S. and Jahanian R. (2017). Effects of dietary inclusion of silymarin on performance, intestinal morphology and ileal bacterial count in aflatoxin-challenged broiler chicks. J. Anim. Physiol. Anim. Nutr. 101(5), 43-54.

Metwally M.A.A., El-Gellal A.M. and El-Sawaisi S.M. (2009). Effects of silymarin on lipid metabolism in rats. World Appl. Sci. J. 6, 1634-16377.

Mousa M.A. and Osman A.S. (2016). The implications of l-carnitine and silymarin supplementation on growth performance and some blood parameters of broilers. Assiut Vet. Med. 148, 132-138.

Muhammad D., Chand N., Khan S., Sultan A., Mushtaq M. and Rafiullah M. (2012). Hepatoprotective role of milk thistle (Silybum marianum) in meat type chicken fed Aflatoxin B1 contaminated feed. Pakistan Vet. J. 9, 102-110.

Puvaca N., Lika E., Cocoli S., Shtylla Kika T., Bursic V., Vukovic G., Tomas Simin M., Petrovic A. and Cara M. (2020). Use of tea tree essential oil (Melaleuca alternifolia) in laying hen’s nutrition on performance and egg fatty acid profile as a promising sustainable organic agricultural tool. Sustainability. 12, 3420-3431.

SAS Institute. (2001). SAS^®/STAT Software, Release 8.2. SAS Institute, Inc., Cary, NC. USA.

Schiavone A., Righi F., Quarantelli A., Bruni R., Serventi P. and Fusari A. (2007). Use of Silybum marianum seed extract in broiler chicken nutrition: influence on performance and meat quality. J. Anim. Physiol. Anim. Nutr. 91, 256-262.

 Shanmugam S., Hong Park J., Sungbo C., and Kim I. (2022). Silymarin seed extract supplementation enhances the growth performance, meat quality, and nutrients digestibility, and reduces gas emission in broilers. Anim. Biosci. 8, 1215-1222.

Stastník O., Juzl M., Karásek F., Štenclová H., Nedomová S., Pavlata L., Mrkvicová E., Doležal P. and Jarošová A. (2016). The effect of feeding milk thistle seed cakes on quality indicators of broiler chickens meat. Potravinarstvo. 10 (1), 248-254.

Suchy P., Strakova E., Kummer V., Herzig I. and Blechova E. (2008). Hepatoprotective effects of milk thistle (Silybum marianum) seed cakes during the chicken broiler fattening. Acta Vet. Brno. 77, 31-38.

Sultan A., Ahmad S., Khan S., Khan R.U., Chand N., Tahir M. and Shakoor A. (2018). Comparative effect of zinc oxide and silymarin on growth, nutrient utilization and hematological parameters of heat distressed broiler. Pakistan J. Zool. 50, 751-756.

Surai P.F. (2015). Silymarin as a Natural Antioxidant: An overview of the current evidence and perspectives. Antioxidants. 4, 204-247.

Tajmohammadi A., Razavi B.M. and Hosseinzadeh H. (2018). Silybum marianum (milk thistle) and its main constituent, silymarin, as a potential therapeutic plant in metabolic syndrome: A review. Photother. Res. 32, 1933-1949.

Tedesco D., Steidler S., Galletti S., Tameni M., Sonzogni O. and Ravarotto L. (2004). Efficacy of silymarin-phospholipid complex in reducing the toxicity of aflatoxin B1 in broiler chickens. Poult. Sci. 83(11), 1839-1843.

Xu Z.R., Hu C.H., Xia M.S., Zhan X.A. and Wang M.Q. (2003). Effects of dietary fructooligosaccharide on digestive enzyme activities, intestinal microflora and morphology of male broilers. Poult. Sci. 82, 1030-1036.

Yi D., Gu L., Ding B., Li M., Hou Y., Wang L. and Gong J. (2012). Effects of dietary silymarin supplementation on growth performance and oxidative status in Carassius auratus gibelio. J. Anim. Vet. Adv. 11, 3399-3404.